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Protein

Neocarzinostatin

Gene

ncsA

Organism
Streptomyces carzinostaticus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

NCS has antibiotic activity (for Gram-positive bacteria) and antitumor activity (for certain mouse tumors). NCS binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neocarzinostatin
Short name:
NCS
Alternative name(s):
Mitomalcin
Short name:
MMC
Gene namesi
Name:ncsA
OrganismiStreptomyces carzinostaticus
Taxonomic identifieri1897 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 342 PublicationsAdd BLAST34
ChainiPRO_000001946135 – 147NeocarzinostatinAdd BLAST113

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi71 ↔ 811 Publication
Disulfide bondi122 ↔ 1271 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi38 – 43Combined sources6
Beta strandi45 – 47Combined sources3
Beta strandi52 – 59Combined sources8
Beta strandi60 – 63Combined sources4
Beta strandi65 – 75Combined sources11
Beta strandi78 – 81Combined sources4
Helixi83 – 85Combined sources3
Beta strandi87 – 90Combined sources4
Turni93 – 95Combined sources3
Beta strandi97 – 102Combined sources6
Beta strandi105 – 110Combined sources6
Beta strandi112 – 114Combined sources3
Beta strandi116 – 121Combined sources6
Turni122 – 124Combined sources3
Beta strandi127 – 132Combined sources6
Beta strandi134 – 136Combined sources3
Beta strandi142 – 145Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J5HNMR-A35-147[»]
1J5INMR-A35-147[»]
1NCOX-ray1.80A/B35-147[»]
1NOAX-ray1.50A35-147[»]
1O5PNMR-A35-147[»]
2CBMX-ray2.03A35-146[»]
2CBOX-ray1.70A35-147[»]
2CBQX-ray2.60A/B/C/D/E/F35-147[»]
2CBTX-ray2.20A/B35-146[»]
2G0KNMR-A35-147[»]
2G0LNMR-A35-147[»]
ProteinModelPortaliP0A3R9.
SMRiP0A3R9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A3R9.

Family & Domainsi

Sequence similaritiesi

Belongs to the neocarzinostatin family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.230. 1 hit.
InterProiIPR027273. Neocarzinostatin-like.
IPR002186. Neocarzinostatin_fam.
[Graphical view]
PfamiPF00960. Neocarzinostat. 1 hit.
[Graphical view]
PRINTSiPR01885. MACROMOMYCIN.
ProDomiPD012709. NCS-type_chromop. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49319. SSF49319. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A3R9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPISIIRNR VAKVAVGSAA VLGLAVGFQT PAVAAAPTAT VTPSSGLSDG
60 70 80 90 100
TVVKVAGAGL QAGTAYDVGQ CAWVDTGVLA CNPADFSSVT ADANGSASTS
110 120 130 140
LTVRRSFEGF LFDGTRWGTV DCTTAACQVG LSDAAGNGPE GVAISFN
Length:147
Mass (Da):14,455
Last modified:March 15, 2005 - v1
Checksum:iC9BE690CC60AC0C6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94N → D AA sequence (PubMed:2938543).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10996 Genomic DNA. Translation: BAA01764.1.
S65575 Genomic DNA. Translation: AAB28103.1.
PIRiJH0792. ZNSMCC.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10996 Genomic DNA. Translation: BAA01764.1.
S65575 Genomic DNA. Translation: AAB28103.1.
PIRiJH0792. ZNSMCC.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J5HNMR-A35-147[»]
1J5INMR-A35-147[»]
1NCOX-ray1.80A/B35-147[»]
1NOAX-ray1.50A35-147[»]
1O5PNMR-A35-147[»]
2CBMX-ray2.03A35-146[»]
2CBOX-ray1.70A35-147[»]
2CBQX-ray2.60A/B/C/D/E/F35-147[»]
2CBTX-ray2.20A/B35-146[»]
2G0KNMR-A35-147[»]
2G0LNMR-A35-147[»]
ProteinModelPortaliP0A3R9.
SMRiP0A3R9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP0A3R9.

Family and domain databases

Gene3Di2.60.40.230. 1 hit.
InterProiIPR027273. Neocarzinostatin-like.
IPR002186. Neocarzinostatin_fam.
[Graphical view]
PfamiPF00960. Neocarzinostat. 1 hit.
[Graphical view]
PRINTSiPR01885. MACROMOMYCIN.
ProDomiPD012709. NCS-type_chromop. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49319. SSF49319. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNCZS_STRCZ
AccessioniPrimary (citable) accession number: P0A3R9
Secondary accession number(s): P01550
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.