Reviewed, UniProtKB/Swiss-Prot P0A3R0 (MSAB1_STRR6)

Names and origin

Protein names

Recommended name:
    Peptide methionine sulfoxide reductase msrA/msrB 1
Including the following 2 domains:
    1- Recommended name:
            Peptide methionine sulfoxide reductase msrA
                Short name=Protein-methionine-S-oxide reductase
              EC=1.8.4.11
        Alternative name(s):
            Peptide-methionine (S)-S-oxide reductase
              Short name=Peptide Met(O) reductase
    2- Recommended name:
            Peptide methionine sulfoxide reductase msrB
              EC=1.8.4.12
        Alternative name(s):
            Peptide-methionine (R)-S-oxide reductase

Gene names

Name:	msrAB1
Synonyms:	exp3, msrA
Ordered Locus Names:	spr1217

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6) [Complete proteome] [HAMAP]

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has an important function as a repair enzyme for proteins that have been inactivated by oxidation By similarity. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.
Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin. Ref.1 L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin. Ref.1 Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin.
Subcellular location	Cell membrane; Peripheral membrane protein Potential.
Disruption phenotype	Mutant exhibits decreased binding to GalNAcbeta1-4Gal containing receptors that are present on type II lung cells and vascular endothelial cells. Ref.1
Sequence similarities	In the N-terminal section; belongs to the msrA Met sulfoxide reductase family. In the C-terminal section; belongs to the msrB Met sulfoxide reductase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Molecular function	Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein modification process Inferred from electronic annotation. Source: HAMAP
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peptide-methionine (R)-S-oxide reductase activity Inferred from electronic annotation. Source: EC peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Peptide methionine sulfoxide reductase msrA/msrB 1 HAMAP MF_01400		PRO_0000138518
Regions
	Region	1 – 155	155	Peptide methionine sulfoxide reductase HAMAP MF_01400
	Region	172 – 295	124	Peptide methionine sulfoxide reductase B HAMAP MF_01400
Sites
	Active site	10	1	By similarity
	Active site	284	1	By similarity
Experimental info
	Sequence conflict	29	1	V → G in AAC44298. Ref.1
	Sequence conflict	45	1	K → E in AAC44298. Ref.1
	Sequence conflict	52 – 57	6	TVQVIY → AVRVIC in AAC44298. Ref.1
	Sequence conflict	61	1	E → G in AAC44298. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A3R0-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 6F3873FCE9C1F6E1	FASTA	312	35,703
	10 20 30 40 50 60 MAEIYLAGGC FWGLEEYFSR ISGVLETSVG YANGQVETTN YQLLKETDHA ETVQVIYDEK 70 80 90 100 110 120 EVSLREILLY YFRVIDPLSI NQQGNDRGRQ YRTGIYYQDE ADLPAIYTVV QEQERMLGRK 130 140 150 160 170 180 IAVEVEQLRH YILAEDYHQD YLRKNPSGYC HIDVTDADKP LIDAANYEKP SQEVLKASLS 190 200 210 220 230 240 EESYRVTQEA ATEAPFTNAY DQTFEEGIYV DITTGEPLFF AKDKFASGCG WPSFSRPISK 250 260 270 280 290 300 ELIHYYKDLS HGMERIEVRS RSGSAHLGHV FTDGPRELGG LRYCINSASL RFVAKDEMEK 310 AGYGYLLPYL NK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Peptide methionine sulfoxide reductase contributes to the maintenance of adhesins in three major pathogens." Wizemann T.M., Moskovitz J., Pearce B.J., Cundell D., Arvidson C.G., So M., Weissbach H., Brot N., Masure H.R. Proc. Natl. Acad. Sci. U.S.A. 93:7985-7990(1996) [PubMed: 8755589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, POSSIBLE FUNCTION IN VIRULENCE, DISRUPTION PHENOTYPE.
[2]	"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed: 11544234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Genetic identification of exported proteins in Streptococcus pneumoniae." Pearce B.J., Yin Y.B., Masure H.R. Mol. Microbiol. 9:1037-1050(1993) [PubMed: 7934910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 76-249.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U41735 Genomic DNA. Translation: AAC44298.1. AE007317 Genomic DNA. Translation: AAL00021.1.
PIR	H98023.
RefSeq	NP_358810.1.
3D structure databases
HSSP	HSSP built from PDB template 1L1D based on UniProtKB P14930.
ModBase	Search...
Genome annotation databases
GeneID	934434.
GenomeReviews	Gene locus spr1217 in contig AE007317_GR.
KEGG	spr:spr1217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A3R0.
OMA	P0A3R0. DERVIYL.
Enzyme and pathway databases
BioCyc	SPNE171101:SPR1217-MON.
Family and domain databases
HAMAP	MF_01400. Fused. [Tree] MF_01401. Fused. [Tree]
InterPro	IPR002579. Methionine_sulphoxide_MsrB. IPR002569. MsrA. [Graphical view]
Gene3D	G3DSA:3.30.1060.10. MsrA. 1 hit. G3DSA:2.170.150.20. MsrB. 1 hit.
Pfam	PF01625. PMSR. 1 hit. PF01641. SelR. 1 hit. [Graphical view]
ProDom	PD004057. DUF25. 1 hit. PD003489. PMSR. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00401. msrA. 1 hit. TIGR00357. MsrB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSAB1_STRR6

Accession

Primary (citable) accession number: P0A3R0
Secondary accession number(s): P35593

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)