P0A3M5 (PBP2_STRPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Penicillin-binding protein 2B

Gene names

Name:	penA
Synonyms:	pbp2b
Ordered Locus Names:	SP_1673

Organism

Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [Complete proteome] [HAMAP]

Taxonomic identifier

170187 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	680 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Subcellular location	Cell membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the transpeptidase family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	penicillin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 680	680	Penicillin-binding protein 2B		PRO_0000195455
Regions
	Transmembrane	9 – 29	21	Helical; Potential
Natural variations
	Natural variant	333	1	E → G in strain: 64147.
	Natural variant	426 – 432	7	TQAYGSF → PAFSRPM in strain: 64147.
	Natural variant	446	1	T → A in strain: 64147.
	Natural variant	476	1	E → G in strain: 64147.
	Natural variant	489	1	T → S in strain: 64147.
	Natural variant	512	1	Y → F in strain: 64147.
	Natural variant	538	1	N → D in strain: 64147.
	Natural variant	597	1	G → E in strain: 64147.
	Natural variant	674 – 676	3	QKY → NQH in strain: 64147.

Sequences

	Sequence		Length	Mass (Da)
	P0A3M5 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 56BF2C1AA9B54C3B	FASTA	680	73,873
	10 20 30 40 50 60 MRKFNSHSIP IRLNLLFSIV ILLFMTIIGR LLYMQVLNKD FYEKKLASAS QTKITSSSAR 70 80 90 100 110 120 GEIYDASGKP LVENTLKQVV SFTRSNKMTA TDLKETAKKL LTYVSISSPN LTERQLADYY 130 140 150 160 170 180 LADPEIYKKI VEALPSEKRL DSDGNRLSES ELYNNAVDSV QTSQLNYTED EKKEIYLFSQ 190 200 210 220 230 240 LNAVGNFATG TIATDPLNDS QVAVIASISK EMPGISISTS WDRKVLETSL SSIVGSVSSE 250 260 270 280 290 300 KAGLPAEEAE AYLKKGYSLN DRVGTSYLEK QYEETLQGKR SVKEIHLDKY GNMESVDTIE 310 320 330 340 350 360 EGSKGNNIKL TIDLAFQDSV DALLKSYFNS ELENGGAKYS EGVYAVALNP KTGAVLSMSG 370 380 390 400 410 420 IKHDLKTGEL TPDSLGTVTN VFVPGSVVKA ATISSGWENG VLSGNQTLTD QSIVFQGSAP 430 440 450 460 470 480 INSWYTQAYG SFPITAVQAL EYSSNTYMVQ TALGLMGQTY QPNMFVGTSN LESAMEKLRS 490 500 510 520 530 540 TFGEYGLGTA TGIDLPDEST GFVPKEYSFA NYITNAFGQF DNYTPMQLAQ YVATIANNGV 550 560 570 580 590 600 RVAPRIVEGI YGNNDKGGLG DLIQQLQPTE MNKVNISDSD MSILHQGFYQ VAHGTSGLTT 610 620 630 640 650 660 GRAFSNGALV SISGKTGTAE SYVADGQQAT NTNAVAYAPS DNPQIAVAVV FPHNTNLTNG 670 680 VGPSIARDII NLYQKYHPMN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.
[2]	"Extensive re-modelling of the transpeptidase domain of penicillin-binding protein 2B of a penicillin-resistant South African isolate of Streptococcus pneumoniae." Dowson C.G., Hutchison A., Spratt B.G. Mol. Microbiol. 3:95-102(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 195-680. Strain: 64147.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005672 Genomic DNA. Translation: AAK75752.1. X13136 Genomic DNA. Translation: CAA31526.1.
PIR	B35965. G95194.
RefSeq	NP_346112.1. NC_003028.3.
3D structure databases
ProteinModelPortal	P0A3M5.
ModBase	Search...
Protein-protein interaction databases
STRING	170187.SP_1673.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK75752; AAK75752; SP_1673.
GeneID	931164.
KEGG	spn:SP_1673.
PATRIC	19707781. VBIStrPne105772_1733.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0768.
HOGENOM	HOG000013470.
KO	K00687.
OMA	GQEANNT.
ProtClustDB	CLSK873017.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR005311. PBP_dimer. IPR001460. PCN-bd_Tpept. [Graphical view]
Pfam	PF03717. PBP_dimer. 1 hit. PF00905. Transpeptidase. 1 hit. [Graphical view]
SUPFAM	SSF56519. PBP_dimer. 1 hit. SSF56601. PBP_transp_fold. 1 hit.
ProtoNet	Search...
Other
ChEMBL	CHEMBL1255133.
DrugBank	DB01163. Amdinocillin.

Entry information

Entry name

PBP2_STRPN

Accession

Primary (citable) accession number: P0A3M5
Secondary accession number(s): P10524

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program