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P0A3M4 (BLO9_ENTAE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase OXA-9

EC=3.5.2.6
Alternative name(s):
Oxacillinase
Penicillinase
Gene names
Name:bla
Synonyms:oxa9
Encoded onPlasmid pIP833
OrganismEnterobacter aerogenes (Aerobacter aerogenes)
Taxonomic identifier548 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Oxacillin-hydrolyzing beta-lactamase. Confers resistance to beta-lactam antibiotics but at a significantly lower level than the TEM bla gene product.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-D beta-lactamase family.

Sequence caution

The sequence AAD22145.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   Technical termPlasmid
Transposable element
Gene Ontology (GO)
   Biological_processantibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

penicillin binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 274250Beta-lactamase OXA-9
PRO_0000017035

Regions

Region206 – 2083Substrate binding By similarity

Sites

Active site581Acyl-ester intermediate By similarity

Amino acid modifications

Modified residue611N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A3M4 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: CA96C211CEFF5F2D

FASTA27430,568
        10         20         30         40         50         60 
MKKILLLHML VFVSATLPIS SVASDEVETL KCTIIADAIT GNTLYETGEC ARRVSPCSSF 

        70         80         90        100        110        120 
KLPLAIMGFD SGILQSPKSP TWELKPEYNP SPRDRTYKQV YPALWQSDSV VWFSQQLTSR 

       130        140        150        160        170        180 
LGVDRFTEYV KKFEYGNQDV SGDSGKHNGL TQSWLMSSLT ISPKEQIQFL LRFVAHKLPV 

       190        200        210        220        230        240 
SEAAYDMAYA TIPQYQAAEG WAVHGKSGSG WLRDNNGKIN ESRPQGWFVG WAEKNGRQVV 

       250        260        270 
FARLEIGKEK SDIPGGSKAR EDILVELPVL MGNK 

« Hide

References

[1]"Characterization of In40 of Enterobacter aerogenes BM2688, a class 1 integron with two new gene cassettes, cmlA2 and qacF."
Ploy M.-C., Courvalin P., Lambert T.
Antimicrob. Agents Chemother. 42:2557-2563(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BM2688-1.
Transposon: In40.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF034958 Genomic DNA. Translation: AAD22145.1. Different initiation.

3D structure databases

ProteinModelPortalP0A3M4.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL1744486.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP0A3M4.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamPF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLO9_ENTAE
AccessionPrimary (citable) accession number: P0A3M4
Secondary accession number(s): P22070
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: January 15, 2008
Last modified: December 11, 2013
This is version 35 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families