P0A3M3 (BLO9_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase OXA-9
EC=3.5.2.6

Alternative name(s):
Oxacillinase
Penicillinase

Gene names

Name:	bla
Synonyms:	oxa9

Encoded on

Plasmid pJMCMW1

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxacillin-hydrolyzing beta-lactamase. Confers resistance to beta-lactam antibiotics but at a significantly lower level than the TEM bla gene product. Ref.4
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-D beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	Plasmid Transposable element
Gene Ontology (GO)
Biological_process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro peptidoglycan-based cell wall biogenesis Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC penicillin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 274	250	Beta-lactamase OXA-9		PRO_0000017036
Regions
	Region	206 – 208	3	Substrate binding By similarity
Sites
	Active site	58	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Modified residue	61	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A3M3 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: CA96C211CEFF5F2D	FASTA	274	30,568
	10 20 30 40 50 60 MKKILLLHML VFVSATLPIS SVASDEVETL KCTIIADAIT GNTLYETGEC ARRVSPCSSF 70 80 90 100 110 120 KLPLAIMGFD SGILQSPKSP TWELKPEYNP SPRDRTYKQV YPALWQSDSV VWFSQQLTSR 130 140 150 160 170 180 LGVDRFTEYV KKFEYGNQDV SGDSGKHNGL TQSWLMSSLT ISPKEQIQFL LRFVAHKLPV 190 200 210 220 230 240 SEAAYDMAYA TIPQYQAAEG WAVHGKSGSG WLRDNNGKIN ESRPQGWFVG WAEKNGRQVV 250 260 270 FARLEIGKEK SDIPGGSKAR EDILVELPVL MGNK

References

[1]	"Sequencing and expression of aadA, bla, and tnpR from the multiresistance transposon Tn1331." Tolmasky M.E. Plasmid 24:218-226(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Transposon: Tn1331.
[2]	Tolmasky M.E. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO N-TERMINUS.
[3]	"Complete nucleotide sequence of Klebsiella pneumoniae multiresistance plasmid pJHCMW1." Sarno R., McGillivary G., Sherratt D.J., Actis L.A., Tolmasky M.E. Antimicrob. Agents Chemother. 46:3422-3427(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Genetic organization of antibiotic resistance genes (aac(6')-Ib, aadA, and oxa9) in the multiresistance transposon Tn1331." Tolmasky M.E., Crosa J.H. Plasmid 29:31-40(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55547 Genomic DNA. Translation: AAA98406.2. AF479774 Genomic DNA. Translation: AAL93143.2.
PIR	D37392.
RefSeq	NP_608309.2. NC_003486.1.
3D structure databases
ProteinModelPortal	P0A3M3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1238791.
Phylogenomic databases
ProtClustDB	CLSK505361.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR002137. Beta-lactam_class-D_AS. IPR001460. PCN-bd_Tpept. [Graphical view]
Pfam	PF00905. Transpeptidase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00337. BETA_LACTAMASE_D. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLO9_KLEPN

Accession

Primary (citable) accession number: P0A3M3
Secondary accession number(s): P22070, Q7WWR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	May 30, 2006
Last modified:	April 3, 2013
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program