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P0A3M3 (BLO9_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase OXA-9

EC=3.5.2.6
Alternative name(s):
Oxacillinase
Penicillinase
Gene names
Name:bla
Synonyms:oxa9
Encoded onPlasmid pJMCMW1
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Oxacillin-hydrolyzing beta-lactamase. Confers resistance to beta-lactam antibiotics but at a significantly lower level than the TEM bla gene product. Ref.4

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-D beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   Technical termPlasmid
Transposable element
Gene Ontology (GO)
   Biological_processantibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

penicillin binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 274250Beta-lactamase OXA-9
PRO_0000017036

Regions

Region206 – 2083Substrate binding By similarity

Sites

Active site581Acyl-ester intermediate By similarity

Amino acid modifications

Modified residue611N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A3M3 [UniParc].

Last modified May 30, 2006. Version 2.
Checksum: CA96C211CEFF5F2D

FASTA27430,568
        10         20         30         40         50         60 
MKKILLLHML VFVSATLPIS SVASDEVETL KCTIIADAIT GNTLYETGEC ARRVSPCSSF 

        70         80         90        100        110        120 
KLPLAIMGFD SGILQSPKSP TWELKPEYNP SPRDRTYKQV YPALWQSDSV VWFSQQLTSR 

       130        140        150        160        170        180 
LGVDRFTEYV KKFEYGNQDV SGDSGKHNGL TQSWLMSSLT ISPKEQIQFL LRFVAHKLPV 

       190        200        210        220        230        240 
SEAAYDMAYA TIPQYQAAEG WAVHGKSGSG WLRDNNGKIN ESRPQGWFVG WAEKNGRQVV 

       250        260        270 
FARLEIGKEK SDIPGGSKAR EDILVELPVL MGNK 

« Hide

References

[1]"Sequencing and expression of aadA, bla, and tnpR from the multiresistance transposon Tn1331."
Tolmasky M.E.
Plasmid 24:218-226(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Transposon: Tn1331.
[2]Tolmasky M.E.
Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO N-TERMINUS.
[3]"Complete nucleotide sequence of Klebsiella pneumoniae multiresistance plasmid pJHCMW1."
Sarno R., McGillivary G., Sherratt D.J., Actis L.A., Tolmasky M.E.
Antimicrob. Agents Chemother. 46:3422-3427(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Genetic organization of antibiotic resistance genes (aac(6')-Ib, aadA, and oxa9) in the multiresistance transposon Tn1331."
Tolmasky M.E., Crosa J.H.
Plasmid 29:31-40(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M55547 Genomic DNA. Translation: AAA98406.2.
AF479774 Genomic DNA. Translation: AAL93143.2.
PIRD37392.
RefSeqNP_608309.2. NC_003486.1.
YP_007878590.1. NC_021079.1.
YP_008603397.1. NC_022520.1.

3D structure databases

ProteinModelPortalP0A3M3.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1238791.
15334410.
17111304.

Phylogenomic databases

ProtClustDBCLSK505361.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamPF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLO9_KLEPN
AccessionPrimary (citable) accession number: P0A3M3
Secondary accession number(s): P22070, Q7WWR7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: May 30, 2006
Last modified: January 22, 2014
This is version 40 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families