P0A3M1 (BLA5_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase SHV-5
EC=3.5.2.6

Gene names

Name:	bla
Synonyms:	shv5

Encoded on

Plasmid pAFF1 Ref.1

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	SHV enzymes hydrolyze broad spectrum cephalosporins notably cefotaxime and ceftazidime. SHV-5 causes particularly high levels of resistance to aztreonam and ceftazidime.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 286	265	Beta-lactamase SHV-5		PRO_0000016983
Regions
	Region	230 – 232	3	Substrate binding By similarity
Sites
	Active site	66	1	Acyl-ester intermediate By similarity
	Active site	164	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	73 ↔ 119		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A3M1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 738F426CC51F5FBA	FASTA	286	31,253
	10 20 30 40 50 60 MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG RTLTAWRADE 70 80 90 100 110 120 RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA 130 140 150 160 170 180 AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTPA 190 200 210 220 230 240 SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGASKRGARG 250 260 270 280 IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR

References

[1]	"Nucleotide sequence of the SHV-5 beta-lactamase gene of a Klebsiella pneumoniae plasmid." Billot-Klein D., Gutmann L., Collatz E. Antimicrob. Agents Chemother. 34:2439-2441(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Survey and molecular genetics of SHV beta-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12." Nuesch-Inderbinen M., Kayser F.H., Hachler H. Antimicrob. Agents Chemother. 41:943-949(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KPGE-2 and KPLA-4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55640 Genomic DNA. Translation: CAA39164.1. X98103 Genomic DNA. Translation: CAA66731.1. X98104 Genomic DNA. Translation: CAA66732.1.
PIR	A60632.
3D structure databases
ProteinModelPortal	P0A3M1.
SMR	P0A3M1. Positions 22-286.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
SABIO-RK	P0A3M1.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P0A3M1.
ChEMBL	CHEMBL1075174.

Entry information

Entry name

BLA5_KLEPN

Accession

Primary (citable) accession number: P0A3M1
Secondary accession number(s): P37320

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	April 3, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program