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P0A3M1 (BLA5_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase SHV-5

EC=3.5.2.6
Gene names
Name:bla
Synonyms:shv5
Encoded onPlasmid pAFF1 Ref.1
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

SHV enzymes hydrolyze broad spectrum cephalosporins notably cefotaxime and ceftazidime. SHV-5 causes particularly high levels of resistance to aztreonam and ceftazidime.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 286265Beta-lactamase SHV-5
PRO_0000016983

Regions

Region230 – 2323Substrate binding By similarity

Sites

Active site661Acyl-ester intermediate By similarity
Active site1641Proton acceptor By similarity

Amino acid modifications

Disulfide bond73 ↔ 119 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A3M1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 738F426CC51F5FBA

FASTA28631,253
        10         20         30         40         50         60 
MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG RTLTAWRADE 

        70         80         90        100        110        120 
RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA 

       130        140        150        160        170        180 
AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTPA 

       190        200        210        220        230        240 
SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGASKRGARG 

       250        260        270        280 
IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR 

« Hide

References

[1]"Nucleotide sequence of the SHV-5 beta-lactamase gene of a Klebsiella pneumoniae plasmid."
Billot-Klein D., Gutmann L., Collatz E.
Antimicrob. Agents Chemother. 34:2439-2441(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Survey and molecular genetics of SHV beta-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12."
Nuesch-Inderbinen M., Kayser F.H., Hachler H.
Antimicrob. Agents Chemother. 41:943-949(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: KPGE-2 and KPLA-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55640 Genomic DNA. Translation: CAA39164.1.
X98103 Genomic DNA. Translation: CAA66731.1.
X98104 Genomic DNA. Translation: CAA66732.1.
PIRA60632.

3D structure databases

ProteinModelPortalP0A3M1.
SMRP0A3M1. Positions 22-286.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP0A3M1.
ChEMBLCHEMBL1075174.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP0A3M1.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLA5_KLEPN
AccessionPrimary (citable) accession number: P0A3M1
Secondary accession number(s): P37320
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: December 11, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families