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P0A3M0

- KITH_SHIFL

UniProt

P0A3M0 - KITH_SHIFL

Protein

Thymidine kinase

Gene

tdk

Organism
Shigella flexneri
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Phosphorylates both thymidine and deoxyuridine.By similarity

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

    Enzyme regulationi

    Allosteric enzyme which is feedback inhibited by dTTP and activated by a number of dNDP and dNTP.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Proton acceptorUniRule annotation
    Metal bindingi145 – 1451ZincUniRule annotation
    Metal bindingi147 – 1471ZincUniRule annotation
    Metal bindingi182 – 1821ZincUniRule annotation
    Metal bindingi185 – 1851ZincUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi9 – 168ATPUniRule annotation
    Nucleotide bindingi87 – 904ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. thymidine kinase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
    Gene namesi
    Name:tdkUniRule annotation
    Ordered Locus Names:SF1238, S1324
    OrganismiShigella flexneri
    Taxonomic identifieri623 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 205205Thymidine kinasePRO_0000175016Add
    BLAST

    Proteomic databases

    PaxDbiP0A3M0.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi198214.SF1238.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A3M0.
    SMRiP0A3M0. Positions 1-189.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidine kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1435.
    HOGENOMiHOG000076391.
    KOiK00857.
    OMAiELKTVCH.
    OrthoDBiEOG69D3J2.

    Family and domain databases

    HAMAPiMF_00124. Thymidine_kinase.
    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A3M0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQLYFYYSA MNAGKSTALL QSSYNYQERG MRTVVYTAEI DDRFGAGKVS    50
    SRIGLSSPAK LFNQNSSLFD EIRAEHEQQA IHCVLVDECQ FLTRQQVYEL 100
    SEVVDQLDIP VLCYGLRTDF RGELFIGSQY LLAWSDKLVE LKTICFCGRK 150
    ASMVLRLDQA GRPYNEGEQV VIGGNERYVS VCRKHYKEAL QVGSLTAIQE 200
    RHRHD 205
    Length:205
    Mass (Da):23,399
    Last modified:March 1, 2005 - v1
    Checksum:i533166D9E91D0E16
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005674 Genomic DNA. Translation: AAN42851.1.
    AE014073 Genomic DNA. Translation: AAP16736.1.
    RefSeqiNP_707144.1. NC_004337.2.
    NP_836929.1. NC_004741.1.

    Genome annotation databases

    EnsemblBacteriaiAAN42851; AAN42851; SF1238.
    AAP16736; AAP16736; S1324.
    GeneIDi1024199.
    1077655.
    KEGGisfl:SF1238.
    sfx:S1324.
    PATRICi18703886. VBIShiFle31049_1447.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005674 Genomic DNA. Translation: AAN42851.1 .
    AE014073 Genomic DNA. Translation: AAP16736.1 .
    RefSeqi NP_707144.1. NC_004337.2.
    NP_836929.1. NC_004741.1.

    3D structure databases

    ProteinModelPortali P0A3M0.
    SMRi P0A3M0. Positions 1-189.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198214.SF1238.

    Proteomic databases

    PaxDbi P0A3M0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN42851 ; AAN42851 ; SF1238 .
    AAP16736 ; AAP16736 ; S1324 .
    GeneIDi 1024199.
    1077655.
    KEGGi sfl:SF1238.
    sfx:S1324.
    PATRICi 18703886. VBIShiFle31049_1447.

    Phylogenomic databases

    eggNOGi COG1435.
    HOGENOMi HOG000076391.
    KOi K00857.
    OMAi ELKTVCH.
    OrthoDBi EOG69D3J2.

    Family and domain databases

    HAMAPi MF_00124. Thymidine_kinase.
    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
      Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
      , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
      Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 301 / Serotype 2a.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700930 / 2457T / Serotype 2a.

    Entry informationi

    Entry nameiKITH_SHIFL
    AccessioniPrimary (citable) accession number: P0A3M0
    Secondary accession number(s): Q9S5G7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3