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P0A3L9

- KITH_ECO57

UniProt

P0A3L9 - KITH_ECO57

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Protein

Thymidine kinase

Gene
tdk, Z2015, ECs1740
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Phosphorylates both thymidine and deoxyuridine By similarity.UniRule annotation

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Enzyme regulationi

Allosteric enzyme which is feedback inhibited by dTTP and activated by a number of dNDP and dNTP By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Proton acceptor Reviewed prediction
Metal bindingi145 – 1451Zinc By similarity
Metal bindingi147 – 1471Zinc By similarity
Metal bindingi182 – 1821Zinc By similarity
Metal bindingi185 – 1851Zinc By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 168ATP By similarity
Nucleotide bindingi87 – 904ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. thymidine kinase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciECOL386585:GJFA-1724-MONOMER.
ECOO157:TDK-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:tdk
Ordered Locus Names:Z2015, ECs1740
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Thymidine kinaseUniRule annotationPRO_0000174973Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi155864.Z2015.

Structurei

3D structure databases

ProteinModelPortaliP0A3L9.
SMRiP0A3L9. Positions 1-189.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine kinase family.

Phylogenomic databases

eggNOGiCOG1435.
HOGENOMiHOG000076391.
KOiK00857.
OMAiELKTVCH.
OrthoDBiEOG69D3J2.

Family and domain databases

HAMAPiMF_00124. Thymidine_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A3L9-1 [UniParc]FASTAAdd to Basket

« Hide

MAQLYFYYSA MNAGKSTALL QSSYNYQERG MRTVVYTAEI DDRFGAGKVS    50
SRIGLSSPAK LFNQNSSLFD EIRAEHEQQA IHCVLVDECQ FLTRQQVYEL 100
SEVVDQLDIP VLCYGLRTDF RGELFIGSQY LLAWSDKLVE LKTICFCGRK 150
ASMVLRLDQA GRPYNEGEQV VIGGNERYVS VCRKHYKEAL QVGSLTAIQE 200
RHRHD 205
Length:205
Mass (Da):23,399
Last modified:March 1, 2005 - v1
Checksum:i533166D9E91D0E16
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB008676 Genomic DNA. Translation: BAA77748.1.
AE005174 Genomic DNA. Translation: AAG56095.1.
BA000007 Genomic DNA. Translation: BAB35163.1.
PIRiC85704.
D90846.
RefSeqiNP_287483.1. NC_002655.2.
NP_309767.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG56095; AAG56095; Z2015.
BAB35163; BAB35163; BAB35163.
GeneIDi913112.
960490.
KEGGiece:Z2015.
ecs:ECs1740.
PATRICi18352744. VBIEscCol44059_1595.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB008676 Genomic DNA. Translation: BAA77748.1 .
AE005174 Genomic DNA. Translation: AAG56095.1 .
BA000007 Genomic DNA. Translation: BAB35163.1 .
PIRi C85704.
D90846.
RefSeqi NP_287483.1. NC_002655.2.
NP_309767.1. NC_002695.1.

3D structure databases

ProteinModelPortali P0A3L9.
SMRi P0A3L9. Positions 1-189.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z2015.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG56095 ; AAG56095 ; Z2015 .
BAB35163 ; BAB35163 ; BAB35163 .
GeneIDi 913112.
960490.
KEGGi ece:Z2015.
ecs:ECs1740.
PATRICi 18352744. VBIEscCol44059_1595.

Phylogenomic databases

eggNOGi COG1435.
HOGENOMi HOG000076391.
KOi K00857.
OMAi ELKTVCH.
OrthoDBi EOG69D3J2.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-1724-MONOMER.
ECOO157:TDK-MONOMER.

Family and domain databases

HAMAPi MF_00124. Thymidine_kinase.
InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the genes responsible for the O-antigen synthesis in enterohaemorrhagic Escherichia coli O157."
    Shimizu T., Yamasaki S., Tsukamoto T., Takeda Y.
    Microb. Pathog. 26:235-247(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: O157:H- / 184 / EHEC.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  3. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiKITH_ECO57
AccessioniPrimary (citable) accession number: P0A3L9
Secondary accession number(s): Q9S5G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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