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P0A3L9

- KITH_ECO57

UniProt

P0A3L9 - KITH_ECO57

Protein

Thymidine kinase

Gene

tdk

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (01 Mar 2005)
      Previous versions | rss
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    Functioni

    Phosphorylates both thymidine and deoxyuridine.By similarity

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

    Enzyme regulationi

    Allosteric enzyme which is feedback inhibited by dTTP and activated by a number of dNDP and dNTP.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Proton acceptorUniRule annotation
    Metal bindingi145 – 1451ZincUniRule annotation
    Metal bindingi147 – 1471ZincUniRule annotation
    Metal bindingi182 – 1821ZincUniRule annotation
    Metal bindingi185 – 1851ZincUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi9 – 168ATPUniRule annotation
    Nucleotide bindingi87 – 904ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. thymidine kinase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-1724-MONOMER.
    ECOO157:TDK-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
    Gene namesi
    Name:tdkUniRule annotation
    Ordered Locus Names:Z2015, ECs1740
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 205205Thymidine kinasePRO_0000174973Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi155864.Z2015.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A3L9.
    SMRiP0A3L9. Positions 1-189.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidine kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1435.
    HOGENOMiHOG000076391.
    KOiK00857.
    OMAiELKTVCH.
    OrthoDBiEOG69D3J2.

    Family and domain databases

    HAMAPiMF_00124. Thymidine_kinase.
    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A3L9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQLYFYYSA MNAGKSTALL QSSYNYQERG MRTVVYTAEI DDRFGAGKVS    50
    SRIGLSSPAK LFNQNSSLFD EIRAEHEQQA IHCVLVDECQ FLTRQQVYEL 100
    SEVVDQLDIP VLCYGLRTDF RGELFIGSQY LLAWSDKLVE LKTICFCGRK 150
    ASMVLRLDQA GRPYNEGEQV VIGGNERYVS VCRKHYKEAL QVGSLTAIQE 200
    RHRHD 205
    Length:205
    Mass (Da):23,399
    Last modified:March 1, 2005 - v1
    Checksum:i533166D9E91D0E16
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB008676 Genomic DNA. Translation: BAA77748.1.
    AE005174 Genomic DNA. Translation: AAG56095.1.
    BA000007 Genomic DNA. Translation: BAB35163.1.
    PIRiC85704.
    D90846.
    RefSeqiNP_287483.1. NC_002655.2.
    NP_309767.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG56095; AAG56095; Z2015.
    BAB35163; BAB35163; BAB35163.
    GeneIDi913112.
    960490.
    KEGGiece:Z2015.
    ecs:ECs1740.
    PATRICi18352744. VBIEscCol44059_1595.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB008676 Genomic DNA. Translation: BAA77748.1 .
    AE005174 Genomic DNA. Translation: AAG56095.1 .
    BA000007 Genomic DNA. Translation: BAB35163.1 .
    PIRi C85704.
    D90846.
    RefSeqi NP_287483.1. NC_002655.2.
    NP_309767.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali P0A3L9.
    SMRi P0A3L9. Positions 1-189.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 155864.Z2015.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG56095 ; AAG56095 ; Z2015 .
    BAB35163 ; BAB35163 ; BAB35163 .
    GeneIDi 913112.
    960490.
    KEGGi ece:Z2015.
    ecs:ECs1740.
    PATRICi 18352744. VBIEscCol44059_1595.

    Phylogenomic databases

    eggNOGi COG1435.
    HOGENOMi HOG000076391.
    KOi K00857.
    OMAi ELKTVCH.
    OrthoDBi EOG69D3J2.

    Enzyme and pathway databases

    BioCyci ECOL386585:GJFA-1724-MONOMER.
    ECOO157:TDK-MONOMER.

    Family and domain databases

    HAMAPi MF_00124. Thymidine_kinase.
    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the genes responsible for the O-antigen synthesis in enterohaemorrhagic Escherichia coli O157."
      Shimizu T., Yamasaki S., Tsukamoto T., Takeda Y.
      Microb. Pathog. 26:235-247(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: O157:H- / 184 / EHEC.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    3. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiKITH_ECO57
    AccessioniPrimary (citable) accession number: P0A3L9
    Secondary accession number(s): Q9S5G7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3