P0A3H0 (DBH_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-binding protein HU

Alternative name(s):
DNA-binding protein II
HB

Gene names

Name:	hup
Synonyms:	hbs, hbsU

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	90 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the bacterial histone-like protein family.

Ontologies

Keywords
Biological process	DNA condensation
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	chromosome condensation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 90

90

DNA-binding protein HU

PRO_0000104912

Amino acid modifications

Modified residue

4

1

Phosphothreonine By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

90

Helix Strand Turn

Details...

Helix

4 – 14

11

Helix

18 – 38

21

Turn

45 – 47

3

Beta strand

48 – 50

3

Beta strand

59 – 61

3

Beta strand

63 – 70

8

Helix

84 – 88

5

Sequences

	Sequence		Length	Mass (Da)
	P0A3H0 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 4F51530D032C071E	FASTA	90	9,716
	10 20 30 40 50 60 MNKTELINAV AETSGLSKKD ATKAVDAVFD SITEALRKGD KVQLIGFGNF EVRERAARKG 70 80 90 RNPQTGEEME IPASKVPAFK PGKALKDAVK

References

[1]	"The DNA-binding protein HU from mesophilic and thermophilic bacilli: gene cloning, overproduction and purification." Padas P.M., Wilson K.S., Vorgias C.E. Gene 117:39-44(1992) [PubMed: 1644313] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning of the gene encoding DNA binding protein HU from Bacillus stearothermophilus and its expression in Escherichia coli." Kawamura S., Kajiyama H., Yamasaki N., Kimura M. Biosci. Biotechnol. Biochem. 59:126-129(1995) [PubMed: 7765961] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1503.
[3]	"On the DNA binding protein II from Bacillus stearothermophilus. II. The amino acid sequence and its relation to those of homologous proteins from other prokaryotes." Kimura M., Wilson K.S. J. Biol. Chem. 258:4007-4011(1983) [PubMed: 6300069] [Abstract] Cited for: PROTEIN SEQUENCE.
[4]	"3-A resolution structure of a protein with histone-like properties in prokaryotes." Tanaka I., Appelt K., Dijk J., White S.W., Wilson K.S. Nature 310:376-381(1984) [PubMed: 6540370] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3 ANGSTROMS).
[5]	"Solution structure of the HU protein from Bacillus stearothermophilus." Vis H., Mariani M., Vorgias C.E., Wilson K.S., Kaptein R., Boelens R. J. Mol. Biol. 254:692-703(1995) [PubMed: 7500343] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M73500 Genomic DNA. Translation: AAA22532.1.
D38080 Genomic DNA. Translation: BAA07273.1.

PIR

DNBS2F. JC1205.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HUE	NMR	-	A/B	1-90	[»]
1HUU	X-ray	2.00	A/B/C	1-90	[»]

ProteinModelPortal

P0A3H0.

SMR

P0A3H0. Positions 1-90.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR023630. DNA-bd_HU/IHF.
IPR000119. Hist_DNA-bd_bac.
IPR020816. Histone-like_DNA-bd_CS.
IPR010992. IHF-like_DNA-bd.
[Graphical view]

Gene3D

G3DSA:4.10.520.10. Hist_DNA_bd_bac. 1 hit.

Pfam

PF00216. Bac_DNA_binding. 1 hit.
[Graphical view]

PRINTS

PR01727. DNABINDINGHU.

SMART

SM00411. BHL. 1 hit.
[Graphical view]

SUPFAM

SSF47729. IHF_like_DNA_bnd. 1 hit.

PROSITE

PS00045. HISTONE_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DBH_GEOSE

Accession

Primary (citable) accession number: P0A3H0
Secondary accession number(s): P02346, P08822

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 31, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program