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P0A3G2

- DHAA_RHORH

UniProt

P0A3G2 - DHAA_RHORH

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Protein

Haloalkane dehalogenase

Gene

dhaA

Organism
Rhodococcus rhodochrous
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Expresses halogenase activity against 1-chloroalkanes of chain length C3 to C10, and also shows a very weak activity with 1,2-dichloroethane.

Catalytic activityi

1-haloalkane + H2O = a primary alcohol + halide.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei106 – 1061NucleophileBy similarity
Active sitei130 – 1301Proton donorBy similarity
Active sitei272 – 2721Proton acceptorBy similarity

GO - Molecular functioni

  1. haloalkane dehalogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. response to toxic substance Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Detoxification

Enzyme and pathway databases

SABIO-RKP0A3G2.
UniPathwayiUPA00007.

Names & Taxonomyi

Protein namesi
Recommended name:
Haloalkane dehalogenase (EC:3.8.1.5)
Gene namesi
Name:dhaA
Encoded oniPlasmid pRTL10 Publication
OrganismiRhodococcus rhodochrous
Taxonomic identifieri1829 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 293293Haloalkane dehalogenasePRO_0000216775Add
BLAST

Expressioni

Inductioni

By 1-haloalkanes.

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 175Combined sources
Beta strandi20 – 289Combined sources
Beta strandi30 – 323Combined sources
Beta strandi35 – 384Combined sources
Helixi45 – 484Combined sources
Turni49 – 513Combined sources
Helixi52 – 554Combined sources
Turni56 – 583Combined sources
Beta strandi61 – 644Combined sources
Helixi81 – 9414Combined sources
Beta strandi99 – 1057Combined sources
Helixi106 – 11813Combined sources
Helixi120 – 1223Combined sources
Beta strandi123 – 1308Combined sources
Beta strandi135 – 1373Combined sources
Helixi138 – 1403Combined sources
Helixi143 – 1453Combined sources
Helixi146 – 1527Combined sources
Beta strandi154 – 1563Combined sources
Helixi157 – 1626Combined sources
Helixi167 – 1704Combined sources
Helixi172 – 1754Combined sources
Beta strandi177 – 1793Combined sources
Helixi183 – 1908Combined sources
Helixi191 – 1933Combined sources
Helixi196 – 1994Combined sources
Helixi200 – 2089Combined sources
Helixi216 – 23116Combined sources
Beta strandi236 – 2438Combined sources
Beta strandi245 – 2473Combined sources
Helixi249 – 25810Combined sources
Beta strandi262 – 27211Combined sources
Helixi274 – 2774Combined sources
Helixi279 – 28911Combined sources
Helixi291 – 2933Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V9ZX-ray3.00A1-11[»]
A142-293[»]
3FBWX-ray1.23A1-293[»]
3RK4X-ray1.31A1-293[»]
3SK0X-ray1.78A1-293[»]
4E46X-ray1.26A1-293[»]
4F5ZX-ray1.20A1-293[»]
4F60X-ray1.45A1-293[»]
4FWBX-ray1.26A4-293[»]
ProteinModelPortaliP0A3G2.
SMRiP0A3G2. Positions 4-293.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A3G2.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01231. Haloalk_dehal_type2.
InterProiIPR029058. AB_hydrolase.
IPR000639. Epox_hydrolase-like.
IPR023594. Haloalkane_dehalogenase_2.
[Graphical view]
PRINTSiPR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A3G2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEIGTGFPF DPHYVEVLGE RMHYVDVGPR DGTPVLFLHG NPTSSYLWRN
60 70 80 90 100
IIPHVAPSHR CIAPDLIGMG KSDKPDLDYF FDDHVRYLDA FIEALGLEEV
110 120 130 140 150
VLVIHDWGSA LGFHWAKRNP ERVKGIACME FIRPIPTWDE WPEFARETFQ
160 170 180 190 200
AFRTADVGRE LIIDQNAFIE GALPKCVVRP LTEVEMDHYR EPFLKPVDRE
210 220 230 240 250
PLWRFPNELP IAGEPANIVA LVEAYMNWLH QSPVPKLLFW GTPGVLIPPA
260 270 280 290
EAARLAESLP NCKTVDIGPG LHYLQEDNPD LIGSEIARWL PAL
Length:293
Mass (Da):33,246
Last modified:March 15, 2005 - v1
Checksum:i2B637C53E36BE9F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060871 Genomic DNA. Translation: AAC15838.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060871 Genomic DNA. Translation: AAC15838.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2V9Z X-ray 3.00 A 1-11 [» ]
A 142-293 [» ]
3FBW X-ray 1.23 A 1-293 [» ]
3RK4 X-ray 1.31 A 1-293 [» ]
3SK0 X-ray 1.78 A 1-293 [» ]
4E46 X-ray 1.26 A 1-293 [» ]
4F5Z X-ray 1.20 A 1-293 [» ]
4F60 X-ray 1.45 A 1-293 [» ]
4FWB X-ray 1.26 A 4-293 [» ]
ProteinModelPortali P0A3G2.
SMRi P0A3G2. Positions 4-293.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00007 .
SABIO-RK P0A3G2.

Miscellaneous databases

EvolutionaryTracei P0A3G2.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
HAMAPi MF_01231. Haloalk_dehal_type2.
InterProi IPR029058. AB_hydrolase.
IPR000639. Epox_hydrolase-like.
IPR023594. Haloalkane_dehalogenase_2.
[Graphical view ]
PRINTSi PR00412. EPOXHYDRLASE.
SUPFAMi SSF53474. SSF53474. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The plasmid-located haloalkane dehalogenase gene from Rhodococcus rhodochrous NCIMB 13064."
    Kulakova A.N., Larkin M.J., Kulakov L.A.
    Microbiology 143:109-115(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NCIMB 13064.

Entry informationi

Entry nameiDHAA_RHORH
AccessioniPrimary (citable) accession number: P0A3G2
Secondary accession number(s): Q53042
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 26, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3