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P0A3G2 (DHAA_RHORH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Haloalkane dehalogenase

EC=3.8.1.5
Gene names
Name:dhaA
Encoded onPlasmid pRTL1
OrganismRhodococcus rhodochrous
Taxonomic identifier1829 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Expresses halogenase activity against 1-chloroalkanes of chain length C3 to C10, and also shows a very weak activity with 1,2-dichloroethane. HAMAP-Rule MF_01231

Catalytic activity

1-haloalkane + H2O = a primary alcohol + halide. HAMAP-Rule MF_01231

Pathway

Xenobiotic degradation; haloalkane degradation. HAMAP-Rule MF_01231

Subunit structure

Monomer.

Induction

By 1-haloalkanes. HAMAP-Rule MF_01231

Sequence similarities

Belongs to the haloalkane dehalogenase family. Type 2 subfamily.

Ontologies

Keywords
   Biological processDetoxification
   Molecular functionHydrolase
   Technical term3D-structure
Plasmid
Gene Ontology (GO)
   Biological_processresponse to toxic substance

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionhaloalkane dehalogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 293293Haloalkane dehalogenase HAMAP-Rule MF_01231
PRO_0000216775

Sites

Active site1061Nucleophile By similarity
Active site1301Proton donor By similarity
Active site2721Proton acceptor By similarity

Secondary structure

............................................................ 293
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A3G2 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 2B637C53E36BE9F3

FASTA29333,246
        10         20         30         40         50         60 
MSEIGTGFPF DPHYVEVLGE RMHYVDVGPR DGTPVLFLHG NPTSSYLWRN IIPHVAPSHR 

        70         80         90        100        110        120 
CIAPDLIGMG KSDKPDLDYF FDDHVRYLDA FIEALGLEEV VLVIHDWGSA LGFHWAKRNP 

       130        140        150        160        170        180 
ERVKGIACME FIRPIPTWDE WPEFARETFQ AFRTADVGRE LIIDQNAFIE GALPKCVVRP 

       190        200        210        220        230        240 
LTEVEMDHYR EPFLKPVDRE PLWRFPNELP IAGEPANIVA LVEAYMNWLH QSPVPKLLFW 

       250        260        270        280        290 
GTPGVLIPPA EAARLAESLP NCKTVDIGPG LHYLQEDNPD LIGSEIARWL PAL 

« Hide

References

[1]"The plasmid-located haloalkane dehalogenase gene from Rhodococcus rhodochrous NCIMB 13064."
Kulakova A.N., Larkin M.J., Kulakov L.A.
Microbiology 143:109-115(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NCIMB 13064.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF060871 Genomic DNA. Translation: AAC15838.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2V9ZX-ray3.00A1-11[»]
A142-293[»]
3FBWX-ray1.23A1-293[»]
3RK4X-ray1.31A1-293[»]
3SK0X-ray1.78A1-293[»]
4E46X-ray1.26A1-293[»]
4F5ZX-ray1.20A1-293[»]
4F60X-ray1.45A1-293[»]
4FWBX-ray1.26A4-293[»]
ProteinModelPortalP0A3G2.
SMRP0A3G2. Positions 4-293.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP0A3G2.
UniPathwayUPA00007.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
HAMAPMF_01231. Haloalk_dehal_type2.
InterProIPR029058. AB_hydrolase.
IPR000639. Epox_hydrolase-like.
IPR023594. Haloalkane_dehalogenase_2.
[Graphical view]
PRINTSPR00412. EPOXHYDRLASE.
SUPFAMSSF53474. SSF53474. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0A3G2.

Entry information

Entry nameDHAA_RHORH
AccessionPrimary (citable) accession number: P0A3G2
Secondary accession number(s): Q53042
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: July 9, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways