P0A3E0 (FLAV_ANASO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flavodoxin

Gene names

Name:

isiB

Organism

Anabaena sp. (strain PCC 7119)

Taxonomic identifier

1168 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Low-potential electron donor to a number of redox enzymes.
Cofactor	FMN.
Sequence similarities	Belongs to the flavodoxin family. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	FMN Flavoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular response to iron ion starvation Traceable author statement. Source: UniProtKB electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Traceable author statement. Source: UniProtKB electron carrier activity Inferred from direct assay. Source: UniProtKB oxidoreductase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FDXR	P08165	3	EBI-593907,EBI-593948	From a different organism.
petH	P21890	5	EBI-593907,EBI-593915

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 170

169

Flavodoxin

PRO_0000171600

Regions

Domain

5 – 165

161

Flavodoxin-like

Secondary structure

1

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170

Helix Strand Turn

Details...

Beta strand

4 – 9

6

Beta strand

12 – 14

3

Helix

15 – 27

13

Turn

29 – 31

3

Beta strand

32 – 36

5

Turn

37 – 39

3

Helix

42 – 47

6

Beta strand

49 – 58

10

Turn

59 – 61

3

Helix

65 – 71

7

Helix

72 – 76

5

Beta strand

83 – 89

7

Turn

92 – 97

6

Helix

101 – 112

12

Beta strand

138 – 144

7

Turn

146 – 148

3

Helix

150 – 152

3

Helix

153 – 168

16

Sequences

	Sequence		Length	Mass (Da)
	P0A3E0 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 069E8DEBA9E33302	FASTA	170	18,964
	10 20 30 40 50 60 MSKKIGLFYG TQTGKTESVA EIIRDEFGND VVTLHDVSQA EVTDLNDYQY LIIGCPTWNI 70 80 90 100 110 120 GELQSDWEGL YSELDDVDFN GKLVAYFGTG DQIGYADNFQ DAIGILEEKI SQRGGKTVGY 130 140 150 160 170 WSTDGYDFND SKALRNGKFV GLALDEDNQS DLTDDRIKSW VAQLKSEFGL

References

[1]	"Isolation and overexpression in Escherichia coli of the flavodoxin gene from Anabaena PCC 7119." Fillat M.F., Borrias W.E., Weisbeek P.J. Biochem. J. 280:187-191(1991) [PubMed: 1720613] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structural and chemical properties of a flavodoxin from Anabaena PCC 7119." Fillat M.F., Edmondson D.E., Gomez-Moreno C. Biochim. Biophys. Acta 1040:301-307(1990) [PubMed: 2119231] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-37.
[3]	"Energetics of a hydrogen bond (charged and neutral) and of a cation-pi interaction in apoflavodoxin." Fernandez-Recio J., Romero A., Sancho J. J. Mol. Biol. 290:319-330(1999) [PubMed: 10388575] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

S68006 Genomic DNA. Translation: AAB20462.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DX9	X-ray	2.05	A/B/C/D	2-170	[»]
1FTG	X-ray	2.00	A	3-169	[»]
1OBO	X-ray	1.20	A/B	3-170	[»]
1OBV	X-ray	2.10	A	3-170	[»]
1QHE	X-ray	2.00	A	3-170	[»]
2KQU	NMR	-	A	3-170	[»]
2V5U	X-ray	1.99	A/B	3-169	[»]
2V5V	X-ray	1.88	A/B	3-170	[»]
3ESX	X-ray	2.31	A/B	2-170	[»]
3ESY	X-ray	2.39	A/B/C/D	2-170	[»]
3ESZ	X-ray	1.94	A/B	5-170	[»]

ProteinModelPortal

P0A3E0.

SMR

P0A3E0. Positions 2-170.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A3E0. 3 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008254. Flavodoxin/NO_synth.
IPR001226. Flavodoxin_CS.
IPR010086. Flavodoxin_lc.
[Graphical view]

Pfam

PF00258. Flavodoxin_1. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01752. Flav_long. 1 hit.

PROSITE

PS00201. FLAVODOXIN. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FLAV_ANASO

Accession

Primary (citable) accession number: P0A3E0
Secondary accession number(s): P11241

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	January 23, 2007
Last modified:	October 19, 2011
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program