P0A3D8 (HEMH_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:	hemH
Ordered Locus Names:	BruAb2_0076

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Ferrochelatase HAMAP MF_00323		PRO_0000175120
Sites
	Metal binding	222	1	Iron By similarity
	Metal binding	303	1	Iron By similarity
Experimental info
	Sequence conflict	208	1	A → V in AAK14798. Ref.2
	Sequence conflict	289	1	L → S in AAK43712. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A3D8 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 4D76D2727A435D2D	FASTA	352	40,083
	10 20 30 40 50 60 MSGTDKVRVN VSQTAQTPLH TSAKLPKVGV LLVNLGTPDG TSYGPMRRYL AEFLSDRRVI 70 80 90 100 110 120 EWSRLIWYPI LYGIVLNTRP RRSGRLYDRI WNHENNESPL RTYTRAQGEK LAKALSDQPN 130 140 150 160 170 180 VVVDWAMRYG QPSIESITDR LLQQGCERIV IFPLYPQYSA TTTATVNDKF FEALMKKRFM 190 200 210 220 230 240 PAIRTVPSYE AEPVYIDALA RSVEKHLATL SFKPEVILTS YHGIPKSYSD KGDPYRQQCL 250 260 270 280 290 300 ETTRLLRERL GLGEDEMRAT FQSRFGPEEW LQPYTDETVK ELAKNGVKLV AVLNPGFVAD 310 320 330 340 350 CLETVDEIGN EAAEEFLENG GENFSHIPCL NDSEEGMKVI ETLVRRELLG WV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Omp10 gene is located upstream of hemH in Brucella." Tibor A., Aidant N., Letesson J.-J. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 544 / Biovar 1.
[2]	"Ferrochelatase is present in Brucella abortus and is critical for its intracellular survival and virulence." Almiron M., Martinez M., Sanjuan N., Ugalde R.A. Infect. Immun. 69:6225-6230(2001) [PubMed: 11553564] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF358663 Genomic DNA. Translation: AAK43712.1. AY027659 Genomic DNA. Translation: AAK14798.1. AE017224 Genomic DNA. Translation: AAX75527.1.
RefSeq	YP_222888.1. NC_006933.1.
3D structure databases
ProteinModelPortal	P0A3D8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3341660.
GenomeReviews	Gene locus BruAb2_0076 in contig AE017224_GR.
KEGG	bmb:BruAb2_0076.
PATRIC	17826345. VBIBruAbo15061_2358.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697135.
OMA	TIEEIGM.
PhylomeDB	P0A3D8.
ProtClustDB	PRK00035.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB2_0076-MONOMER.
BRENDA	4.99.1.1. 994.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BRUAB

Accession

Primary (citable) accession number: P0A3D8
Secondary accession number(s): Q57A07, Q939N8, Q93TG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program