Reviewed, UniProtKB/Swiss-Prot P0A3C8 (FER1_ANASO)

Names and origin

Protein names

Recommended name:
    Ferredoxin-1
Alternative name(s):
    Ferredoxin I

Gene names

Name:	petF
Synonyms:	fdxV

Organism

Anabaena sp. (strain PCC 7119)

Taxonomic identifier

1168 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	99 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
Cofactor	Binds 1 2Fe-2S cluster.
Sequence similarities	Belongs to the 2Fe2S plant-type ferredoxin family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
narB	P39458	1	EBI-637080,EBI-932578	From a different organism.
petH	P21890	5	EBI-637080,EBI-593915

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 99

98

Ferredoxin-1

PRO_0000189299

Regions

Domain

4 – 96

93

2Fe-2S ferredoxin-type

Sites

Metal binding

42

1

Iron-sulfur (2Fe-2S)

Metal binding

47

1

Iron-sulfur (2Fe-2S)

Metal binding

50

1

Iron-sulfur (2Fe-2S)

Metal binding

80

1

Iron-sulfur (2Fe-2S)

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

99

Helix Strand Turn

Details...

Beta strand

3 – 10

8

Turn

11 – 14

4

Beta strand

15 – 22

8

Helix

27 – 33

7

Beta strand

41 – 48

8

Beta strand

51 – 57

7

Helix

69 – 73

5

Beta strand

76 – 78

3

Helix

79 – 81

3

Beta strand

83 – 86

4

Beta strand

88 – 91

4

Turn

95 – 98

4

Sequences

	Sequence		Length	Mass (Da)
	P0A3C8-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 874FD6365346BFFF	FASTA	99	10,830
	10 20 30 40 50 60 MATFKVTLIN EAEGTKHEIE VPDDEYILDA AEEQGYDLPF SCRAGACSTC AGKLVSGTVD 70 80 90 QSDQSFLDDD QIEAGYVLTC VAYPTSDVVI QTHKEEDLY

References

[1]	"Refined X-ray structures of the oxidized, at 1.3 A, and reduced, at 1.17 A, [2Fe-2S] ferredoxin from the cyanobacterium anabaena PCC7119 show redox-linked conformational changes." Morales R., Charon M.-H., Hudry-Clergeon G., Petillot Y., Norager S., Medina M., Frey M. Biochemistry 38:15764-15773(1999) [PubMed: 10625442] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-99, X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS).
[2]	"Crystallographic studies of the interaction between the ferredoxin-NADP+ reductase and ferredoxin from the cyanobacterium Anabaena: looking for the elusive ferredoxin molecule." Morales R., Kachalova G., Vellieux F., Charon M.-H., Frey M. Acta Crystallogr. D 56:1408-1412(2000) [PubMed: 11053838] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CZP	X-ray	1.17	A/B	2-99	[»]
1EWY	X-ray	2.38	C	2-99	[»]
1QT9	X-ray	1.30	A	2-99	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A3C8. 2 interactions.

Family and domain databases

InterPro

IPR006058. 2Fe2S_fd_BS.
IPR012675. b-grasp_ferredoxin-like.
IPR010241. Fdx_pln.
IPR001041. Ferredoxin.
[Graphical view]

Gene3D

G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.

Pfam

PF00111. Fer2. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02008. fdx_plant. 1 hit.

PROSITE

PS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FER1_ANASO

Accession

Primary (citable) accession number: P0A3C8
Secondary accession number(s): P06543

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)