Chlorocatechol 1,2-dioxygenase - Ralstonia eutropha (strain JMP134)

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Reviewed, UniProtKB/Swiss-Prot P0A396 (TFDC_RALEJ)

Last modified March 2, 2010. Version 36. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and originHide

Protein names

Recommended name:
Chlorocatechol 1,2-dioxygenase
EC=1.13.11.-

Gene names

Name:	tfdC
Synonyms:	tfdCI
Ordered Locus Names:	Reut_D6466

Encoded on

Plasmid pJP4 Ref.1 Ref.2 Ref.3 Ref.4
Plasmid pReut1 Ref.5

Organism

Ralstonia eutropha (strain JMP134) (Alcaligenes eutrophus) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

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Protein attributesHide

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

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General annotation (Comments)Hide

Function	Preferentially converts 3,5-dichlorocatechol as opposed to other chlorinated catechols. Retains diminished activity toward non-chlorinated substrates.
Catalytic activity	3,5-dichlorocatechol + O₂ = 2,4-dichloro -muconate.
Cofactor	Binds 1 Fe³⁺ ion per subunit.
Pathway	Aromatic compound metabolism; 3-chlorocatechol degradation.
Sequence similarities	Belongs to the intradiol ring-cleavage dioxygenase family.

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OntologiesHide

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW catechol metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catechol 1,2-dioxygenase activity Inferred from electronic annotation. Source: InterPro chlorocatechol 1,2-dioxygenase activity Inferred from electronic annotation. Source: InterPro ferric iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)Hide

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 255

255

Chlorocatechol 1,2-dioxygenase

PRO_0000085090

Sites

Metal binding

130

Iron By similarity

Metal binding

164

Iron By similarity

Metal binding

188

Iron By similarity

Metal binding

190

Iron By similarity

Experimental info

Sequence conflict

115 – 116

ED → DH Ref.2

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SequencesHide

Sequence

Length

Mass (Da)

Tools

P0A396-1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 5BCC1BC6A94B19B4
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FASTA

255

28,283

        10         20         30         40         50         60 
MNKRVKDVVD AIVAAVQRVL DQKEVTEAEY RTAVHYLMQV AEQRETALLC DVFFNSTVAA 

        70         80         90        100        110        120 
TKARISEGST PAIEGPYYRD DAPLVDDRLK TYDTDDHKPL LIQGTVKAVD GSVVEDVTID 

       130        140        150        160        170        180 
VWHSTPDGKY SGFHDDIPTD FYRGKLRVGT DGSFRVRTTM PVPYQIPDQG PTGALLETMG 

       190        200        210        220        230        240 
GHSWRPAHVH FKVKAPGYET LTTQYYFEGG DWITDDCCNG VQSSLITPDI VEEGVRLMNI 

       250 
NFVIEPARAQ AGANP

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ReferencesHide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4." Perkins E.J., Gordon M.P., Caceres O., Lurquin P.F. J. Bacteriol. 172:2351-2359(1990) [PubMed: 2185214] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Partial nucleotide sequence of the chlorocatechol degradative operon tfdCDEF of pJP4 and similarity to promoters of the chlorinated aromatic degradative operons tfdA and clcABD." Perkins E.J., Bolton G., Gordon M.P., Lurquin P.F. Nucleic Acids Res. 16:7200-7200(1988) [PubMed: 3405772] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Nucleotide homology and organization of chlorocatechol oxidation genes of plasmids pJP4 and pAC27." Ghosal D., You I.-S. Mol. Gen. Genet. 211:113-120(1988) [PubMed: 2830460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways." Trefault N., De la Iglesia R., Molina A.M., Manzano M., Ledger T., Perez-Pantoja D., Sanchez M.A., Stuardo M., Gonzalez B. Environ. Microbiol. 6:655-668(2004) [PubMed: 15186344] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Complete sequence of plasmid 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	M35097 Genomic DNA. Translation: AAA98262.1. M36280 Genomic DNA. Translation: AAA98261.1. AY365053 Genomic DNA. Translation: AAR31039.1. CP000093 Genomic DNA. Translation: AAZ65764.1.
PIR	A35255.
RefSeq	YP_025387.1. YP_293621.1.
3D structure databases
SMR	P0A396. Positions 2-247.
ModBase	Search...
Genome annotation databases
GeneID	2847422. 3607312.
GenomeReviews	Gene locus Reut_D6466 in contig CP000093_GR.
KEGG	reu:Reut_D6466.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG429220.
OMA	QFAPGIP.
ProtClustDB	CLSK926386.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14417. REUT264198:REUT_D6466-MONOMER.
Family and domain databases
InterPro	IPR007535. Catechol_dOase_N. IPR012817. Chlorcchol_dOase. IPR000627. Intradiol_dOase_C. IPR015889. Intradiol_dOase_core. [Graphical view]
Gene3D	G3DSA:2.60.130.10. Intradiol_dOase_core. 1 hit.
Pfam	PF00775. Dioxygenase_C. 1 hit. PF04444. Dioxygenase_N. 1 hit. [Graphical view]
SUPFAM	SSF49482. Dioxygenase. 1 hit.
TIGRFAMs	TIGR02465. chlorocat_1_2. 1 hit.
PROSITE	PS00083. INTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry informationHide

Entry name

TFDC_RALEJ

Accession

Primary (citable) accession number: P0A396
Secondary accession number(s): P05403, P71131, Q46M66

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	March 2, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documentsHide

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and originHide

Protein attributesHide

General annotation (Comments)Hide

OntologiesHide

Sequence annotation (Features)Hide

Molecule processing

Sites

Experimental info

SequencesHide

ReferencesHide

Cross-referencesHide

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry informationHide

Relevant documentsHide