Chlorocatechol 1,2-dioxygenase - Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus)

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P0A396 (TFDC_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 56. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chlorocatechol 1,2-dioxygenase
EC=1.13.11.-

Gene names

Name:	tfdC
Synonyms:	tfdCI
Ordered Locus Names:	Reut_D6466

Encoded on

Plasmid pJP4 Ref.1 Ref.2 Ref.3 Ref.4
Plasmid pReut1 Ref.5

Organism

Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

264198 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus ›

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Preferentially converts 3,5-dichlorocatechol as opposed to other chlorinated catechols. Retains diminished activity toward non-chlorinated substrates.
Catalytic activity	3,5-dichlorocatechol + O₂ = 2,4-dichloro -muconate.
Cofactor	Binds 1 Fe³⁺ ion per subunit.
Pathway	Aromatic compound metabolism; 3-chlorocatechol degradation.
Sequence similarities	Belongs to the intradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW catechol-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	catechol 1,2-dioxygenase activity Inferred from electronic annotation. Source: InterPro chlorocatechol 1,2-dioxygenase activity Inferred from electronic annotation. Source: InterPro ferric iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 255

255

Chlorocatechol 1,2-dioxygenase

PRO_0000085090

Sites

Metal binding

130

Iron By similarity

Metal binding

164

Iron By similarity

Metal binding

188

Iron By similarity

Metal binding

190

Iron By similarity

Experimental info

Sequence conflict

115 – 116

ED → DH Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P0A396 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 5BCC1BC6A94B19B4
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FASTA

255

28,283

        10         20         30         40         50         60 
MNKRVKDVVD AIVAAVQRVL DQKEVTEAEY RTAVHYLMQV AEQRETALLC DVFFNSTVAA 

        70         80         90        100        110        120 
TKARISEGST PAIEGPYYRD DAPLVDDRLK TYDTDDHKPL LIQGTVKAVD GSVVEDVTID 

       130        140        150        160        170        180 
VWHSTPDGKY SGFHDDIPTD FYRGKLRVGT DGSFRVRTTM PVPYQIPDQG PTGALLETMG 

       190        200        210        220        230        240 
GHSWRPAHVH FKVKAPGYET LTTQYYFEGG DWITDDCCNG VQSSLITPDI VEEGVRLMNI 

       250 
NFVIEPARAQ AGANP

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4." Perkins E.J., Gordon M.P., Caceres O., Lurquin P.F. J. Bacteriol. 172:2351-2359(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Partial nucleotide sequence of the chlorocatechol degradative operon tfdCDEF of pJP4 and similarity to promoters of the chlorinated aromatic degradative operons tfdA and clcABD." Perkins E.J., Bolton G., Gordon M.P., Lurquin P.F. Nucleic Acids Res. 16:7200-7200(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Nucleotide homology and organization of chlorocatechol oxidation genes of plasmids pJP4 and pAC27." Ghosal D., You I.-S. Mol. Gen. Genet. 211:113-120(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways." Trefault N., De la Iglesia R., Molina A.M., Manzano M., Ledger T., Perez-Pantoja D., Sanchez M.A., Stuardo M., Gonzalez B. Environ. Microbiol. 6:655-668(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Complete sequence of plasmid 1 of Ralstonia eutropha JMP134." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JMP134 / LMG 1197.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M35097 Genomic DNA. Translation: AAA98262.1. M36280 Genomic DNA. Translation: AAA98261.1. AY365053 Genomic DNA. Translation: AAR31039.1. CP000093 Genomic DNA. Translation: AAZ65764.1.
PIR	A35255.
RefSeq	YP_025387.1. NC_005912.1. YP_293621.1. NC_007337.1.
3D structure databases
ProteinModelPortal	P0A396.
ModBase	Search...
Protein-protein interaction databases
STRING	264198.Reut_D6466.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAZ65764; AAZ65764; Reut_D6466.
GeneID	2847422. 3607312.
KEGG	reu:Reut_D6466.
PATRIC	20225308. VBIRalEut24049_0533.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3485.
HOGENOM	HOG000150200.
KO	K15247.
OMA	RVRTTMP.
ProtClustDB	CLSK926386.
Enzyme and pathway databases
BioCyc	CPIN264198:GIW3-6552-MONOMER. MetaCyc:MONOMER-14417.
UniPathway	UPA00083.
Family and domain databases
Gene3D	2.60.130.10. 1 hit.
InterPro	IPR007535. Catechol_dOase_N. IPR012817. Chlorcchol_dOase. IPR000627. Intradiol_dOase_C. IPR015889. Intradiol_dOase_core. [Graphical view]
Pfam	PF00775. Dioxygenase_C. 1 hit. PF04444. Dioxygenase_N. 1 hit. [Graphical view]
SUPFAM	SSF49482. Dioxygenase. 1 hit.
TIGRFAMs	TIGR02465. chlorocat_1_2. 1 hit.
PROSITE	PS00083. INTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TFDC_CUPPJ

Accession

Primary (citable) accession number: P0A396
Secondary accession number(s): P05403, P71131, Q46M66

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents