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P0A394 (DHLO_AGRT4) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Protein ocs

Including the following 2 domains:

  1. D-lysopine dehydrogenase
    EC=1.5.1.16
    Alternative name(s):
    Lysopine synthase
  2. D-octopine dehydrogenase
    EC=1.5.1.11
    Alternative name(s):
    Octopine synthase
Gene names
Name:ocs
Encoded onPlasmid pTiAch5
OrganismAgrobacterium tumefaciens (strain Ach5)
Taxonomic identifier176298 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Reductive condensation of pyruvate and arginine, lysine, histidine, or octopine to form octopine, lysopine, histopine, or octopinic acid, respectively. NADPH is the preferred cofactor, but NADH can also be used.

Catalytic activity

N(2)-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH.

N(2)-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O = L-lysine + pyruvate + NADPH.

Subunit structure

Monomer.

Sequence similarities

Belongs to the lysopine/nopaline/octopine/opine/vitopine dehydrogenases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 358358Protein ocs
PRO_0000179978

Sequences

Sequence LengthMass (Da)Tools
P0A394 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: C57D977E4EC4D803

FASTA35838,797
        10         20         30         40         50         60 
MAKVAILGAG NVALTLAGDL ARRLGQVSSI WAPISNRNSF NSVRSLGSLE LVGPDYGGDF 

        70         80         90        100        110        120 
QPQLEDDLET AISGAAFIFL TVPTMGQQGI LCELANFNLS SSVLVALPGS ATSLACKQTL 

       130        140        150        160        170        180 
TPAFAPIAVI EATTSPYACR RVNAQVLMLS VKRTFEVAST QALSEEVRGG FEILFPNRLQ 

       190        200        210        220        230        240 
WYQNPASIFF SNTNPVAHPA GILAAKDTIE QGISPIPKFY RKFVPQAITR VTAIDEERLT 

       250        260        270        280        290        300 
IVNALGLESE TDFAYCKKWY GGHASNAREF YETFEGYADI ETPRNMNHRY LSEDVKHILV 

       310        320        330        340        350 
LWVEIAEVIG VQVPEMKSVV QEASDVLNED LSHTGRGLSS LNLEGSNANA IVRALNGV 

« Hide

References

[1]"Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene."
de Greve H., Dhaese P., Seurinck J., Lemmers M., van Montagu M., Schell J.
J. Mol. Appl. Genet. 1:499-511(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00088 Genomic DNA. Translation: CAA23428.1.
PIRDEAGLT. A00399.
RefSeqNP_059680.1. NC_002377.1.

3D structure databases

ProteinModelPortalP0A394.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1224199.

Phylogenomic databases

ProtClustDBCLSK2459457.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
IPR003421. Opine_DH.
[Graphical view]
PfamPF01210. NAD_Gly3P_dh_N. 1 hit.
PF02317. Octopine_DH. 1 hit.
[Graphical view]
SUPFAMSSF48179. 6DGDH_C_like. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDHLO_AGRT4
AccessionPrimary (citable) accession number: P0A394
Secondary accession number(s): P00385
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 3, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families