P0A394 (DHLO_AGRT4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein ocs

Including the following 2 domains:

1. D-lysopine dehydrogenase
   EC=1.5.1.16
   Alternative name(s):
   Lysopine synthase
2. D-octopine dehydrogenase
   EC=1.5.1.11
   Alternative name(s):
   Octopine synthase

Gene names

Name:

ocs

Encoded on

Plasmid pTiAch5

Organism

Agrobacterium tumefaciens (strain Ach5)

Taxonomic identifier

176298 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › Rhizobium radiobacter

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reductive condensation of pyruvate and arginine, lysine, histidine, or octopine to form octopine, lysopine, histopine, or octopinic acid, respectively. NADPH is the preferred cofactor, but NADH can also be used.
Catalytic activity	N(2)-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH. N(2)-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O = L-lysine + pyruvate + NADPH.
Subunit structure	Monomer.
Sequence similarities	Belongs to the lysopine/nopaline/octopine/opine/vitopine dehydrogenases family.

Ontologies

Keywords
Disease	Crown gall tumor
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	D-lysopine dehydrogenase activity Inferred from electronic annotation. Source: EC D-octopine dehydrogenase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Protein ocs		PRO_0000179978

Sequences

	Sequence		Length	Mass (Da)
	P0A394 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: C57D977E4EC4D803	FASTA	358	38,797
	10 20 30 40 50 60 MAKVAILGAG NVALTLAGDL ARRLGQVSSI WAPISNRNSF NSVRSLGSLE LVGPDYGGDF 70 80 90 100 110 120 QPQLEDDLET AISGAAFIFL TVPTMGQQGI LCELANFNLS SSVLVALPGS ATSLACKQTL 130 140 150 160 170 180 TPAFAPIAVI EATTSPYACR RVNAQVLMLS VKRTFEVAST QALSEEVRGG FEILFPNRLQ 190 200 210 220 230 240 WYQNPASIFF SNTNPVAHPA GILAAKDTIE QGISPIPKFY RKFVPQAITR VTAIDEERLT 250 260 270 280 290 300 IVNALGLESE TDFAYCKKWY GGHASNAREF YETFEGYADI ETPRNMNHRY LSEDVKHILV 310 320 330 340 350 LWVEIAEVIG VQVPEMKSVV QEASDVLNED LSHTGRGLSS LNLEGSNANA IVRALNGV

References

[1]

"Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene." de Greve H., Dhaese P., Seurinck J., Lemmers M., van Montagu M., Schell J. J. Mol. Appl. Genet. 1:499-511(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	V00088 Genomic DNA. Translation: CAA23428.1.
PIR	DEAGLT. A00399.
RefSeq	NP_059680.1. NC_002377.1.
3D structure databases
ProteinModelPortal	P0A394.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1224199.
Phylogenomic databases
ProtClustDB	CLSK2459457.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR011128. G3P_DH_NAD-dep_N. IPR016040. NAD(P)-bd_dom. IPR003421. Opine_DH. [Graphical view]
Pfam	PF01210. NAD_Gly3P_dh_N. 1 hit. PF02317. Octopine_DH. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DHLO_AGRT4

Accession

Primary (citable) accession number: P0A394
Secondary accession number(s): P00385

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program