Reviewed,
UniProtKB/Swiss-Prot P0A394 (DHLO_AGRT4)
Last modified
November 25, 2008.
Version 23.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Protein ocs Including the following 2 domains: 1- Recommended name: D-lysopine dehydrogenase EC=1.5.1.16 Alternative name(s): Lysopine synthase 2- Recommended name: D-octopine dehydrogenase EC=1.5.1.11 Alternative name(s): Octopine synthase | ||
| Gene names |
| ||
| Encoded on | Plasmid pTiAch5 | ||
| Organism | Agrobacterium tumefaciens (strain Ach5) | ||
| Taxonomic identifier | 176298 [NCBI] | ||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium |
Protein attributes
| Sequence length | 358 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Reductive condensation of pyruvate and arginine, lysine, histidine, or octopine to form octopine, lysopine, histopine, or octopinic acid, respectively. NADPH is the preferred cofactor, but NADH can also be used. |
| Catalytic activity | N(2)-(D-1-carboxyethyl)-L-arginine + NAD(+) + H(2)O = L-arginine + pyruvate + NADH. N(2)-(D-1-carboxyethyl)-L-lysine + NADP(+) + H(2)O = L-lysine + pyruvate + NADPH. |
| Subunit structure | Monomer. |
| Sequence similarities | Belongs to the lysopine/nopaline/octopine/opine/vitopine dehydrogenases family. |
Ontologies
Keywords | |
|---|---|
| Disease | Crown gall tumor |
| Ligand | NAD NADP |
| Molecular function | Oxidoreductase |
| Technical term | Plasmid |
Gene Ontology (GO) | |
| Biological process | glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: InterPro |
| Molecular function | D-lysopine dehydrogenase activity Inferred from electronic annotation. Source: EC D-octopine dehydrogenase activityInferred from electronic annotation. Source: EC NAD bindingInferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptorInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 358 | 358 | Protein ocs | PRO_0000179978 | |||
Sequences
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References
| [1] | "Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene." de Greve H., Dhaese P., Seurinck J., Lemmers M., van Montagu M., Schell J. J. Mol. Appl. Genet. 1:499-511(1982) [PubMed: 7153687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| V00088 Genomic DNA. Translation: CAA23428.1. | |
| PIR | DEAGLT. A00399. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1BG6 based on UniProtKB Q44297. |
| ModBase | Search... |
Family and domain databases | |
| InterPro | IPR013328. DHase_multihelical. IPR016040. NAD(P)-bd. IPR011128. NAD-dep_Gly3P_DHase_N. IPR003421. Opine_DHase. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit. |
| Pfam | PF01210. NAD_Gly3P_dh_N. 1 hit. PF02317. Octopine_DH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DHLO_AGRT4 | ||||||||
| Accession | Primary (citable) accession number: P0A394 Secondary accession number(s): P00385 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

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