Reviewed, UniProtKB/Swiss-Prot P0A393 (DHLE_BACCE)

Names and origin

Protein names

Recommended name:
    Leucine dehydrogenase
      Short name=LeuDH
    EC=1.4.1.9

Gene names

Name:

ldh

Organism

Bacillus cereus

Taxonomic identifier

1396 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase By similarity.
Catalytic activity	L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH.
Pathway	Amino-acid degradation; L-leucine degradation; 4-methyl-2-oxopentanoic acid from L-leucine (dehydrogenase route): step 1/1.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Biological process	Branched-chain amino acid catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	branched chain family amino acid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro leucine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Leucine dehydrogenase		PRO_0000182800
Regions
	Nucleotide binding	182 – 188	7	NAD Potential
Sites
	Active site	82	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A393-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: DA84E58062E772AC	FASTA	366	39,867
	10 20 30 40 50 60 MTLEIFEYLE KYDYEQVVFC QDKESGLKAI IAIHDTTLGP ALGGTRMWTY DSEEAAIEDA 70 80 90 100 110 120 LRLAKGMTYK NAAAGLNLGG AKTVIIGDPR KDKSEAMFRA LGRYIQGLNG RYITAEDVGT 130 140 150 160 170 180 TVDDMDIIHE ETDFVTGISP SFGSSGNPSP VTAYGVYRGM KAAAKEAFGT DNLEGKVIAV 190 200 210 220 230 240 QGVGNVAYHL CKHLHAEGAK LIVTDINKEA VQRAVEEFGA SAVEPNEIYG VECDIYAPCA 250 260 270 280 290 300 LGATVNDETI PQLKAKVIAG SANNQLKEDR HGDIIHEMGI VYAPDYVINA GGVINVADEL 310 320 330 340 350 360 YGYNRERALK RVESIYDTIA KVIEISKRDG IATYVAADRL AEERIASLKN SRSTYLRNGH DIISRR

References

[1]

"Cloning, sequencing and overexpression of the leucine dehydrogenase gene from Bacillus cereus." Stoyan T., Recktenwald A., Kula M.R. J. Biotechnol. 54:77-80(1997) [PubMed: 9188201] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18; 47-56; 160-192; 214-254 AND 279-298. Strain: DSM 626.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U51099 Genomic DNA. Translation: AAA96314.1.
3D structure databases
HSSP	HSSP built from PDB template 1C1D based on UniProtKB Q59771.
SMR	P0A393. Positions 3-366.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.9. 604.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR016211. Glu/Phe/Leu/Val_DH_bac/arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000188. Phe_leu_dh. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHLE_BACCE

Accession

Primary (citable) accession number: P0A393
Secondary accession number(s): Q59194

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)