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P0A393 (DHLE_BACCE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine dehydrogenase

Short name=LeuDH
EC=1.4.1.9
Gene names
Name:ldh
OrganismBacillus cereus
Taxonomic identifier1396 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase By similarity.

Catalytic activity

L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH.

Pathway

Amino-acid degradation; L-leucine degradation; 4-methyl-2-oxopentanoate from L-leucine (dehydrogenase route): step 1/1.

Subunit structure

Homohexamer By similarity.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   Biological processBranched-chain amino acid catabolism
   LigandNAD
   Molecular functionOxidoreductase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processleucine catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionleucine dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366Leucine dehydrogenase
PRO_0000182800

Regions

Nucleotide binding182 – 1887NAD Potential

Sites

Active site821 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A393 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: DA84E58062E772AC

FASTA36639,867
        10         20         30         40         50         60 
MTLEIFEYLE KYDYEQVVFC QDKESGLKAI IAIHDTTLGP ALGGTRMWTY DSEEAAIEDA 

        70         80         90        100        110        120 
LRLAKGMTYK NAAAGLNLGG AKTVIIGDPR KDKSEAMFRA LGRYIQGLNG RYITAEDVGT 

       130        140        150        160        170        180 
TVDDMDIIHE ETDFVTGISP SFGSSGNPSP VTAYGVYRGM KAAAKEAFGT DNLEGKVIAV 

       190        200        210        220        230        240 
QGVGNVAYHL CKHLHAEGAK LIVTDINKEA VQRAVEEFGA SAVEPNEIYG VECDIYAPCA 

       250        260        270        280        290        300 
LGATVNDETI PQLKAKVIAG SANNQLKEDR HGDIIHEMGI VYAPDYVINA GGVINVADEL 

       310        320        330        340        350        360 
YGYNRERALK RVESIYDTIA KVIEISKRDG IATYVAADRL AEERIASLKN SRSTYLRNGH 


DIISRR 

« Hide

References

[1]"Cloning, sequencing and overexpression of the leucine dehydrogenase gene from Bacillus cereus."
Stoyan T., Recktenwald A., Kula M.R.
J. Biotechnol. 54:77-80(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18; 47-56; 160-192; 214-254 AND 279-298.
Strain: DSM 626.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U51099 Genomic DNA. Translation: AAA96314.1.

3D structure databases

ProteinModelPortalP0A393.
SMRP0A393. Positions 3-366.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00363; UER00858.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR016211. Glu/Phe/Leu/Val_DH_bac/arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11606:SF3. PTHR11606:SF3. 1 hit.
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000188. Phe_leu_dh. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHLE_BACCE
AccessionPrimary (citable) accession number: P0A393
Secondary accession number(s): Q59194
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: October 16, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways