P0A392 (DHLE_BACCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine dehydrogenase
Short name=LeuDH
EC=1.4.1.9

Gene names

Name:	ldh
Ordered Locus Names:	BC_4162

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31)

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase By similarity.
Catalytic activity	L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH.
Pathway	Amino-acid degradation; L-leucine degradation; 4-methyl-2-oxopentanoate from L-leucine (dehydrogenase route): step 1/1.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Biological process	Branched-chain amino acid catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	leucine dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Leucine dehydrogenase		PRO_0000182801
Regions
	Nucleotide binding	182 – 188	7	NAD Potential
Sites
	Active site	82	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A392 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: DA84E58062E772AC	FASTA	366	39,867
	10 20 30 40 50 60 MTLEIFEYLE KYDYEQVVFC QDKESGLKAI IAIHDTTLGP ALGGTRMWTY DSEEAAIEDA 70 80 90 100 110 120 LRLAKGMTYK NAAAGLNLGG AKTVIIGDPR KDKSEAMFRA LGRYIQGLNG RYITAEDVGT 130 140 150 160 170 180 TVDDMDIIHE ETDFVTGISP SFGSSGNPSP VTAYGVYRGM KAAAKEAFGT DNLEGKVIAV 190 200 210 220 230 240 QGVGNVAYHL CKHLHAEGAK LIVTDINKEA VQRAVEEFGA SAVEPNEIYG VECDIYAPCA 250 260 270 280 290 300 LGATVNDETI PQLKAKVIAG SANNQLKEDR HGDIIHEMGI VYAPDYVINA GGVINVADEL 310 320 330 340 350 360 YGYNRERALK RVESIYDTIA KVIEISKRDG IATYVAADRL AEERIASLKN SRSTYLRNGH DIISRR

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14579 / DSM 31.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP11078.1.
RefSeq	NP_833877.1. NC_004722.1.
3D structure databases
ProteinModelPortal	P0A392.
SMR	P0A392. Positions 3-366.
ModBase	Search...
Protein-protein interaction databases
STRING	P0A392.
Proteomic databases
PRIDE	P0A392.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000034515; EBBACP00000033721; EBBACG00000034506.
GeneID	1206507.
GenomeReviews	Gene locus BC_4162 in contig AE016877_GR.
KEGG	bce:BC4162.
PATRIC	32604398. VBIBacCer54481_4302.
Phylogenomic databases
eggNOG	COG0334.
GeneTree	EBGT00050000001221.
HOGENOM	HBG590661.
OMA	FRAFGRY.
PhylomeDB	P0A392.
ProtClustDB	CLSK873417.
Enzyme and pathway databases
BioCyc	BCER226900:BC_4162-MONOMER.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR016211. Glu/Phe/Leu/Val_DH_bac/arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00263.
PANTHER	PTHR11606:SF3. PTHR11606:SF3. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000188. Phe_leu_dh. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHLE_BACCR

Accession

Primary (citable) accession number: P0A392
Secondary accession number(s): Q59194

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program