P0A382 (CT1AA_BACTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-1Aa cytolytic delta-endotoxin

Alternative name(s):
27 kDa cytolytic toxin

Gene names

Name:	cyt1Aa
Synonyms:	cytA

Encoded on

Plasmid 72 Kb

Organism

Bacillus thuringiensis subsp. israelensis

Taxonomic identifier

1430 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus thuringiensis

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Kills the larvae of dipteran insects by making pores in the epithelial cell membrane of the insect midgut. Acts on mosquitos and black flies.
Developmental stage	The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.
Post-translational modification	Active after proteolytic processing.
Sequence similarities	Belongs to the cyt1/cyt2 endotoxin family.

Ontologies

Keywords
Biological process	Sporulation
Molecular function	Toxin
Technical term	3D-structure Plasmid
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 249

249

Type-1Aa cytolytic delta-endotoxin

PRO_0000174104

Secondary structure

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249

Helix Strand Turn

Details...

Beta strand

40 – 47

8

Helix

49 – 51

3

Helix

52 – 62

11

Helix

63 – 65

3

Beta strand

66 – 68

3

Turn

71 – 73

3

Beta strand

77 – 79

3

Helix

81 – 90

10

Beta strand

96 – 111

16

Helix

112 – 127

16

Helix

129 – 134

6

Helix

136 – 147

12

Helix

151 – 153

3

Beta strand

159 – 165

7

Beta strand

168 – 181

14

Turn

182 – 186

5

Beta strand

187 – 201

15

Helix

203 – 206

4

Beta strand

214 – 229

16

Sequences

	Sequence		Length	Mass (Da)
	P0A382 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: E661946298D3989A	FASTA	249	27,341
	10 20 30 40 50 60 MENLNHCPLE DIKVNPWKTP QSTARVITLR VEDPNEINNL LSINEIDNPN YILQAIMLAN 70 80 90 100 110 120 AFQNALVPTS TDFGDALRFS MPKGLEIANT ITPMGAVVSY VDQNVTQTNN QVSVMINKVL 130 140 150 160 170 180 EVLKTVLGVA LSGSVIDQLT AAVTNTFTNL NTQKNEAWIF WGKETANQTN YTYNVLFAIQ 190 200 210 220 230 240 NAQTGGVMYC VPVGFEIKVS AVKEQVLFFT IQDSASYNVN IQSLKFAQPL VSSSQYPIAD LTSAINGTL

References

[1]	"Bacillus thuringiensis var. israelensis delta-endotoxin. Nucleotide sequence and characterization of the transcripts in Bacillus thuringiensis and Escherichia coli." Ward E.S., Ellar D.J. J. Mol. Biol. 191:1-11(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bacillus thuringiensis var. israelensis delta-endotoxin. Cloning and expression of the toxin in sporogenic and asporogenic strains of Bacillus subtilis." Ward E.S., Ridley A.R., Ellar D.J., Todd J.A. J. Mol. Biol. 191:13-22(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Single amino acid changes in the Bacillus thuringiensis var. israelensis delta-endotoxin affect the toxicity and expression of the protein." Ward E.S., Ellar D.J., Chilcott C.N. J. Mol. Biol. 202:527-535(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS.
[4]	"Molecular cloning and the nucleotide sequence of the Mr 28 000 crystal protein gene of Bacillus thuringiensis subsp. israelensis." Waalwijk C., Dullemans A.M., van Workum M.E.S., Visser B. Nucleic Acids Res. 13:8207-8217(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	Waalwijk C. Submitted (FEB-1986) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 34-35.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X04338 Genomic DNA. Translation: CAA27868.1.
X03182 Genomic DNA. Translation: CAA26943.1.

PIR

A24044.

RefSeq

YP_001573774.1. NC_010076.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J2L	model	-	A	18-249	[»]
3RON	X-ray	2.19	A/B	1-249	[»]

ProteinModelPortal

P0A382.

ModBase

Search...

Protein family/group databases

TCDB

1.C.71.1.1. cytolytic delta endotoxin (Cyt1/2) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

5759908.

Family and domain databases

Gene3D

3.40.198.10. 1 hit.

InterPro

IPR001615. Endotoxin_CytB.
[Graphical view]

Pfam

PF01338. Bac_thur_toxin. 1 hit.
[Graphical view]

ProDom

PD009844. Endotoxin_CytB. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF55676. Endotoxin_CytB. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0A382.

Entry information

Entry name

CT1AA_BACTI

Accession

Primary (citable) accession number: P0A382
Secondary accession number(s): P05069, P05628

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program