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P0A382

- CT1AA_BACTI

UniProt

P0A382 - CT1AA_BACTI

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Protein
Type-1Aa cytolytic delta-endotoxin
Gene
cyt1Aa, cytA
Organism
Bacillus thuringiensis subsp. israelensis
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Kills the larvae of dipteran insects by making pores in the epithelial cell membrane of the insect midgut. Acts on mosquitos and black flies.

GO - Biological processi

  1. pathogenesis Source: InterPro
  2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Sporulation

Protein family/group databases

TCDBi1.C.71.1.1. the cytolytic delta endotoxin (cyt1/2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-1Aa cytolytic delta-endotoxin
Alternative name(s):
27 kDa cytolytic toxin
Gene namesi
Name:cyt1Aa
Synonyms:cytA
Encoded oniPlasmid 72 Kb0 Publication
OrganismiBacillus thuringiensis subsp. israelensis
Taxonomic identifieri1430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249Type-1Aa cytolytic delta-endotoxin
PRO_0000174104Add
BLAST

Post-translational modificationi

Active after proteolytic processing.

Expressioni

Developmental stagei

The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi40 – 478
Helixi49 – 513
Helixi52 – 6211
Helixi63 – 653
Beta strandi66 – 683
Turni71 – 733
Beta strandi77 – 793
Helixi81 – 9010
Beta strandi96 – 11116
Helixi112 – 12716
Helixi129 – 1346
Helixi136 – 14712
Helixi151 – 1533
Beta strandi159 – 1657
Beta strandi168 – 18114
Turni182 – 1865
Beta strandi187 – 20115
Helixi203 – 2064
Beta strandi214 – 22916

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J2Lmodel-A18-249[»]
3RONX-ray2.19A/B1-249[»]
ProteinModelPortaliP0A382.

Miscellaneous databases

EvolutionaryTraceiP0A382.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.198.10. 1 hit.
InterProiIPR001615. Endotoxin_CytB.
[Graphical view]
PfamiPF01338. Bac_thur_toxin. 1 hit.
[Graphical view]
ProDomiPD009844. Endotoxin_CytB. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55676. SSF55676. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A382-1 [UniParc]FASTAAdd to Basket

« Hide

MENLNHCPLE DIKVNPWKTP QSTARVITLR VEDPNEINNL LSINEIDNPN    50
YILQAIMLAN AFQNALVPTS TDFGDALRFS MPKGLEIANT ITPMGAVVSY 100
VDQNVTQTNN QVSVMINKVL EVLKTVLGVA LSGSVIDQLT AAVTNTFTNL 150
NTQKNEAWIF WGKETANQTN YTYNVLFAIQ NAQTGGVMYC VPVGFEIKVS 200
AVKEQVLFFT IQDSASYNVN IQSLKFAQPL VSSSQYPIAD LTSAINGTL 249
Length:249
Mass (Da):27,341
Last modified:August 13, 1987 - v1
Checksum:iE661946298D3989A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04338 Genomic DNA. Translation: CAA27868.1.
X03182 Genomic DNA. Translation: CAA26943.1.
PIRiA24044.
RefSeqiWP_000429377.1. NC_010076.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04338 Genomic DNA. Translation: CAA27868.1 .
X03182 Genomic DNA. Translation: CAA26943.1 .
PIRi A24044.
RefSeqi WP_000429377.1. NC_010076.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2J2L model - A 18-249 [» ]
3RON X-ray 2.19 A/B 1-249 [» ]
ProteinModelPortali P0A382.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

TCDBi 1.C.71.1.1. the cytolytic delta endotoxin (cyt1/2) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P0A382.

Family and domain databases

Gene3Di 3.40.198.10. 1 hit.
InterProi IPR001615. Endotoxin_CytB.
[Graphical view ]
Pfami PF01338. Bac_thur_toxin. 1 hit.
[Graphical view ]
ProDomi PD009844. Endotoxin_CytB. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF55676. SSF55676. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Bacillus thuringiensis var. israelensis delta-endotoxin. Nucleotide sequence and characterization of the transcripts in Bacillus thuringiensis and Escherichia coli."
    Ward E.S., Ellar D.J.
    J. Mol. Biol. 191:1-11(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Bacillus thuringiensis var. israelensis delta-endotoxin. Cloning and expression of the toxin in sporogenic and asporogenic strains of Bacillus subtilis."
    Ward E.S., Ridley A.R., Ellar D.J., Todd J.A.
    J. Mol. Biol. 191:13-22(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Single amino acid changes in the Bacillus thuringiensis var. israelensis delta-endotoxin affect the toxicity and expression of the protein."
    Ward E.S., Ellar D.J., Chilcott C.N.
    J. Mol. Biol. 202:527-535(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS.
  4. "Molecular cloning and the nucleotide sequence of the Mr 28 000 crystal protein gene of Bacillus thuringiensis subsp. israelensis."
    Waalwijk C., Dullemans A.M., van Workum M.E.S., Visser B.
    Nucleic Acids Res. 13:8207-8217(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. Waalwijk C.
    Submitted (FEB-1986) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 34-35.

Entry informationi

Entry nameiCT1AA_BACTI
AccessioniPrimary (citable) accession number: P0A382
Secondary accession number(s): P05069, P05628
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: September 3, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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