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P0A382

- CT1AA_BACTI

UniProt

P0A382 - CT1AA_BACTI

Protein

Type-1Aa cytolytic delta-endotoxin

Gene

cyt1Aa

Organism
Bacillus thuringiensis subsp. israelensis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    Kills the larvae of dipteran insects by making pores in the epithelial cell membrane of the insect midgut. Acts on mosquitos and black flies.

    GO - Biological processi

    1. pathogenesis Source: InterPro
    2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Toxin

    Keywords - Biological processi

    Sporulation

    Protein family/group databases

    TCDBi1.C.71.1.1. the cytolytic delta endotoxin (cyt1/2) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-1Aa cytolytic delta-endotoxin
    Alternative name(s):
    27 kDa cytolytic toxin
    Gene namesi
    Name:cyt1Aa
    Synonyms:cytA
    Encoded oniPlasmid 72 Kb0 Publication
    OrganismiBacillus thuringiensis subsp. israelensis
    Taxonomic identifieri1430 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 249249Type-1Aa cytolytic delta-endotoxinPRO_0000174104Add
    BLAST

    Post-translational modificationi

    Active after proteolytic processing.

    Expressioni

    Developmental stagei

    The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

    Structurei

    Secondary structure

    1
    249
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi40 – 478
    Helixi49 – 513
    Helixi52 – 6211
    Helixi63 – 653
    Beta strandi66 – 683
    Turni71 – 733
    Beta strandi77 – 793
    Helixi81 – 9010
    Beta strandi96 – 11116
    Helixi112 – 12716
    Helixi129 – 1346
    Helixi136 – 14712
    Helixi151 – 1533
    Beta strandi159 – 1657
    Beta strandi168 – 18114
    Turni182 – 1865
    Beta strandi187 – 20115
    Helixi203 – 2064
    Beta strandi214 – 22916

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2J2Lmodel-A18-249[»]
    3RONX-ray2.19A/B1-249[»]
    ProteinModelPortaliP0A382.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A382.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cyt1/cyt2 endotoxin family.Curated

    Family and domain databases

    Gene3Di3.40.198.10. 1 hit.
    InterProiIPR001615. Endotoxin_CytB.
    [Graphical view]
    PfamiPF01338. Bac_thur_toxin. 1 hit.
    [Graphical view]
    ProDomiPD009844. Endotoxin_CytB. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF55676. SSF55676. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A382-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENLNHCPLE DIKVNPWKTP QSTARVITLR VEDPNEINNL LSINEIDNPN    50
    YILQAIMLAN AFQNALVPTS TDFGDALRFS MPKGLEIANT ITPMGAVVSY 100
    VDQNVTQTNN QVSVMINKVL EVLKTVLGVA LSGSVIDQLT AAVTNTFTNL 150
    NTQKNEAWIF WGKETANQTN YTYNVLFAIQ NAQTGGVMYC VPVGFEIKVS 200
    AVKEQVLFFT IQDSASYNVN IQSLKFAQPL VSSSQYPIAD LTSAINGTL 249
    Length:249
    Mass (Da):27,341
    Last modified:August 13, 1987 - v1
    Checksum:iE661946298D3989A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04338 Genomic DNA. Translation: CAA27868.1.
    X03182 Genomic DNA. Translation: CAA26943.1.
    PIRiA24044.
    RefSeqiWP_000429377.1. NC_010076.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04338 Genomic DNA. Translation: CAA27868.1 .
    X03182 Genomic DNA. Translation: CAA26943.1 .
    PIRi A24044.
    RefSeqi WP_000429377.1. NC_010076.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2J2L model - A 18-249 [» ]
    3RON X-ray 2.19 A/B 1-249 [» ]
    ProteinModelPortali P0A382.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    TCDBi 1.C.71.1.1. the cytolytic delta endotoxin (cyt1/2) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P0A382.

    Family and domain databases

    Gene3Di 3.40.198.10. 1 hit.
    InterProi IPR001615. Endotoxin_CytB.
    [Graphical view ]
    Pfami PF01338. Bac_thur_toxin. 1 hit.
    [Graphical view ]
    ProDomi PD009844. Endotoxin_CytB. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF55676. SSF55676. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Bacillus thuringiensis var. israelensis delta-endotoxin. Nucleotide sequence and characterization of the transcripts in Bacillus thuringiensis and Escherichia coli."
      Ward E.S., Ellar D.J.
      J. Mol. Biol. 191:1-11(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Bacillus thuringiensis var. israelensis delta-endotoxin. Cloning and expression of the toxin in sporogenic and asporogenic strains of Bacillus subtilis."
      Ward E.S., Ridley A.R., Ellar D.J., Todd J.A.
      J. Mol. Biol. 191:13-22(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Single amino acid changes in the Bacillus thuringiensis var. israelensis delta-endotoxin affect the toxicity and expression of the protein."
      Ward E.S., Ellar D.J., Chilcott C.N.
      J. Mol. Biol. 202:527-535(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS.
    4. "Molecular cloning and the nucleotide sequence of the Mr 28 000 crystal protein gene of Bacillus thuringiensis subsp. israelensis."
      Waalwijk C., Dullemans A.M., van Workum M.E.S., Visser B.
      Nucleic Acids Res. 13:8207-8217(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. Waalwijk C.
      Submitted (FEB-1986) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 34-35.

    Entry informationi

    Entry nameiCT1AA_BACTI
    AccessioniPrimary (citable) accession number: P0A382
    Secondary accession number(s): P05069, P05628
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3