ID   CR1AB_BACTK             Reviewed;        1155 AA.
AC   P0A370; P06577; P06578; P09663; P09666; P09667; P21257; Q45789;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   24-JAN-2024, entry version 68.
DE   RecName: Full=Pesticidal crystal protein Cry1Ab;
DE   AltName: Full=130 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryIA(b);
GN   Name=cry1Ab; Synonyms=bt2, cry-1-2, cry1A(b), cryIA(b), cryIC1;
OS   Bacillus thuringiensis subsp. kurstaki.
OG   Plasmid pUCBtS93.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=29339;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HD-1;
RX   PubMed=3557124; DOI=10.1016/0378-1119(86)90357-4;
RA   Geiser M., Schweitzer S., Grimm C.;
RT   "The hypervariable region in the genes coding for entomopathogenic crystal
RT   proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene
RT   of subsp. kurstaki HD1.";
RL   Gene 48:109-118(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HD-1;
RX   PubMed=3011746; DOI=10.1128/jb.166.3.801-811.1986;
RA   Thorne L., Garduno F., Thompson T., Decker D., Zounes M., Wild M.,
RA   Walfield A.M., Pollock T.J.;
RT   "Structural similarity between the lepidoptera- and diptera-specific
RT   insecticidal endotoxin genes of Bacillus thuringiensis subsp. 'kurstaki'
RT   and 'israelensis'.";
RL   J. Bacteriol. 166:801-811(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HD-1;
RX   AGRICOLA=IND87016057;
RA   Kondo S., Tamura N., Kunitate A., Hattori M., Akashi A., Ohmori I.;
RT   "Cloning and nucleotide sequencing of two insecticidal delta-endotoxin
RT   genes from Bacillus thuringiensis var. kurstaki HD-1 DNA.";
RL   Agric. Biol. Chem. 51:455-463(1987).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HD-1;
RX   DOI=10.1038/nbt0887-807;
RA   Fischhoff D.A., Bowdish K.S., Perlak F.J., Marrone P.G., McCormick S.M.,
RA   Niedermeyer J.G., Dean D.A., Kusano-Kretzmer K., Mayer E.J.,
RA   Rochester D.E., Rogers S.G., Fraley R.T.;
RT   "Insect tolerant transgenic tomato plants.";
RL   Biotechnology (N.Y.) 5:807-813(1987).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRD-12;
RX   DOI=10.1016/0168-1656(87)90061-7;
RA   Hefford M.A., Brousseau R., Prefontaine G., Hanna Z., Condie J.A.,
RA   Lau P.C.K.;
RT   "Sequence of a lepidopteran toxin gene of Bacillus thuringiensis subsp
RT   kurstaki NRD-12.";
RL   J. Biotechnol. 6:307-322(1987).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S93; PLASMID=pUCBtS93;
RX   DOI=10.1139/w99-032;
RA   Silva-Werneck J.O., De-Souza M.T., Dias J.M.C.S., Ribeiro B.M.;
RT   "Characterization of Bacillus thuringiensis subsp. kurstaki strain S93
RT   effective against the Fall armyworm, Spodoptera frugiperda and cloning of a
RT   cry1Ab gene.";
RL   Can. J. Microbiol. 45:464-471(1999).
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of many lepidopteran larvae.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- BIOTECHNOLOGY: Introduced by genetic manipulation and expressed in
CC       insect-resistant maize by Monsanto, Northrup King and Ciba Geigy.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- MISCELLANEOUS: In strain S93 this is encoded on plasmid pUCBtS39.
CC       {ECO:0000269|Ref.6}.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; M15271; AAA22561.1; -; Genomic_DNA.
DR   EMBL; M12661; AAA22613.1; -; Genomic_DNA.
DR   EMBL; D00117; BAA00071.1; -; Genomic_DNA.
DR   EMBL; M37263; AAA22420.1; -; Genomic_DNA.
DR   EMBL; AF059670; AAC64003.1; -; Genomic_DNA.
DR   EMBL; A03793; CAA00303.1; -; Unassigned_DNA.
DR   EMBL; A09398; CAA00840.1; -; Unassigned_DNA.
DR   PIR; A29125; A29125.
DR   PIR; A29838; A29838.
DR   PIR; A90025; JD0002.
DR   PIR; I39838; I39838.
DR   RefSeq; WP_000369819.1; NZ_PGEH01000375.1.
DR   AlphaFoldDB; P0A370; -.
DR   SMR; P0A370; -.
DR   TCDB; 1.C.2.1.3; the channel-forming Delta-endotoxin insecticidal crystal protein (icp) family.
DR   GeneID; 67470639; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001907; P:killing by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   CDD; cd04085; delta_endotoxin_C; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR   Gene3D; 2.100.10.10; Pesticidal crystal protein, central domain; 1.
DR   Gene3D; 1.20.190.10; Pesticidal crystal protein, N-terminal domain; 1.
DR   InterPro; IPR048645; Cry1Ac-like_dom-VII.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003:SF2; ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR37003; ENDOTOXIN_N DOMAIN-CONTAINING PROTEIN-RELATED; 1.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF21463; Cry1Ac_dom-VII; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF18449; Endotoxin_C2; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF51096; delta-Endotoxin (insectocide), middle domain; 1.
DR   SUPFAM; SSF56849; delta-Endotoxin (insectocide), N-terminal domain; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE   1: Evidence at protein level;
KW   Genetically modified food; Plasmid; Sporulation; Toxin; Virulence.
FT   CHAIN           1..1155
FT                   /note="Pesticidal crystal protein Cry1Ab"
FT                   /id="PRO_0000174023"
FT   CONFLICT        207
FT                   /note="A -> R (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        382..406
FT                   /note="VLDGTEFAYGTSSNLPSAVYRKSGT -> GPDGGRICLWNLLKFGQPPYTEK
FT                   AEP (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        410
FT                   /note="L -> P (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        430..432
FT                   /note="LSH -> CLAY (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        437
FT                   /note="R -> Y (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        447
FT                   /note="I -> V (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        452..458
FT                   /note="MFSWIHR -> NDSSWTYC (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        461
FT                   /note="E -> N (in Ref. 5; AAA22420)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        463..465
FT                   /note="NNI -> GDV (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        479..486
FT                   /note="STNLGSGT -> LQSWLWN (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        492
FT                   /note="P -> L (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        501..505
FT                   /note="RRTSP -> EELT (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        542
FT                   /note="D -> H (in Ref. 5; AAA22420)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        568..569
FT                   /note="TV -> HL (in Ref. 5; AAA22420)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        665
FT                   /note="K -> E (in Ref. 5; AAA22420)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        675..676
FT                   /note="KR -> NG (in Ref. 5; AAA22420)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        703
FT                   /note="S -> N (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        712
FT                   /note="D -> H (in Ref. 2; AAA22613)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        832
FT                   /note="A -> E (in Ref. 4; no nucleotide entry)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        836
FT                   /note="N -> I (in Ref. 5; AAA22420)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        842..843
FT                   /note="EK -> GRA (in Ref. 4; no nucleotide entry)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1016
FT                   /note="E -> T (in Ref. 5; AAA22420)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1060
FT                   /note="E -> G (in Ref. 5; AAA22420)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1155 AA;  130624 MW;  43461A64C7AC7CAF CRC64;
     MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL
     VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD
     PTNPALREEM RIQFNDMNSA LTTAIPLFAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ
     RWGFDAATIN SRYNDLTRLI GNYTDHAVRW YNTGLERVWG PDSRDWIRYN QFRRELTLTV
     LDIVSLFPNY DSRTYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIEGS IRSPHLMDIL
     NSITIYTDAH RGEYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI VAQLGQGVYR
     TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV YRKSGTVDSL DEIPPQNNNV
     PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA PMFSWIHRSA EFNNIIPSSQ ITQIPLTKST
     NLGSGTSVVK GPGFTGGDIL RRTSPGQIST LRVNITAPLS QRYRVRIRYA STTNLQFHTS
     IDGRPINQGN FSATMSSGSN LQSGSFRTVG FTTPFNFSNG SSVFTLSAHV FNSGNEVYID
     RIEFVPAEVT FEAEYDLERA QKAVNELFTS SNQIGLKTDV TDYHIDQVSN LVECLSDEFC
     LDEKKELSEK VKHAKRLSDE RNLLQDPNFR GINRQLDRGW RGSTDITIQG GDDVFKENYV
     TLLGTFDECY PTYLYQKIDE SKLKAYTRYQ LRGYIEDSQD LEIYLIRYNA KHETVNVPGT
     GSLWPLSAPS PIGKCAHHSH HFSLDIDVGC TDLNEDLGVW VIFKIKTQDG HARLGNLEFL
     EEKPLVGEAL ARVKRAEKKW RDKREKLEWE TNIVYKEAKE SVDALFVNSQ YDRLQADTNI
     AMIHAADKRV HSIREAYLPE LSVIPGVNAA IFEELEGRIF TAFSLYDARN VIKNGDFNNG
     LSCWNVKGHV DVEEQNNHRS VLVVPEWEAE VSQEVRVCPG RGYILRVTAY KEGYGEGCVT
     IHEIENNTDE LKFSNCVEEE VYPNNTVTCN DYTATQEEYE GTYTSRNRGY DGAYESNSSV
     PADYASAYEE KAYTDGRRDN PCESNRGYGD YTPLPAGYVT KELEYFPETD KVWIEIGETE
     GTFIVDSVEL LLMEE
//