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P0A370 (CR1AB_BACTK) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pesticidal crystal protein cry1Ab
Alternative name(s):
130 kDa crystal protein
Crystaline entomocidal protoxin
Insecticidal delta-endotoxin CryIA(b)
Gene names
Name:cry1Ab
Synonyms:bt2, cry-1-2, cry1A(b), cryIA(b), cryIC1
Encoded onPlasmid
OrganismBacillus thuringiensis subsp. kurstaki
Taxonomic identifier29339 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length1155 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

Developmental stage

The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

Biotechnological use

Introduced by genetic manipulation and expressed in insect-resistant maize by Monsanto, Northrup King and Ciba Geigy.

Miscellaneous

Toxic segment of the protein is located in the N-terminus.

Sequence similarities

Belongs to the delta endotoxin family.

Ontologies

Keywords
   Biological processSporulation
   Molecular functionToxin
   Technical termGenetically modified food
Plasmid
Gene Ontology (GO)
   Biological_processdefense response

Inferred from electronic annotation. Source: InterPro

pathogenesis

Inferred from electronic annotation. Source: InterPro

sporulation resulting in formation of a cellular spore

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11551155Pesticidal crystal protein cry1Ab
PRO_0000174023

Experimental info

Sequence conflict2071A → R in AAA22613. Ref.2
Sequence conflict382 – 40625VLDGT…RKSGT → GPDGGRICLWNLLKFGQPPY TEKAEP in AAA22613. Ref.2
Sequence conflict4101L → P in AAA22613. Ref.2
Sequence conflict430 – 4323LSH → CLAY in AAA22613. Ref.2
Sequence conflict4371R → Y in AAA22613. Ref.2
Sequence conflict4471I → V in AAA22613. Ref.2
Sequence conflict452 – 4587MFSWIHR → NDSSWTYC in AAA22613. Ref.2
Sequence conflict4611E → N in AAA22420. Ref.5
Sequence conflict463 – 4653NNI → GDV in AAA22613. Ref.2
Sequence conflict479 – 4868STNLGSGT → LQSWLWN in AAA22613. Ref.2
Sequence conflict4921P → L in AAA22613. Ref.2
Sequence conflict501 – 5055RRTSP → EELT in AAA22613. Ref.2
Sequence conflict5421D → H in AAA22420. Ref.5
Sequence conflict568 – 5692TV → HL in AAA22420. Ref.5
Sequence conflict6651K → E in AAA22420. Ref.5
Sequence conflict675 – 6762KR → NG in AAA22420. Ref.5
Sequence conflict7031S → N in AAA22613. Ref.2
Sequence conflict7121D → H in AAA22613. Ref.2
Sequence conflict8321A → E Ref.4
Sequence conflict8361N → I in AAA22420. Ref.5
Sequence conflict842 – 8432EK → GRA Ref.4
Sequence conflict10161E → T in AAA22420. Ref.5
Sequence conflict10601E → G in AAA22420. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P0A370 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 43461A64C7AC7CAF

FASTA1,155130,624
        10         20         30         40         50         60 
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL 

        70         80         90        100        110        120 
VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD 

       130        140        150        160        170        180 
PTNPALREEM RIQFNDMNSA LTTAIPLFAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ 

       190        200        210        220        230        240 
RWGFDAATIN SRYNDLTRLI GNYTDHAVRW YNTGLERVWG PDSRDWIRYN QFRRELTLTV 

       250        260        270        280        290        300 
LDIVSLFPNY DSRTYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIEGS IRSPHLMDIL 

       310        320        330        340        350        360 
NSITIYTDAH RGEYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI VAQLGQGVYR 

       370        380        390        400        410        420 
TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV YRKSGTVDSL DEIPPQNNNV 

       430        440        450        460        470        480 
PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA PMFSWIHRSA EFNNIIPSSQ ITQIPLTKST 

       490        500        510        520        530        540 
NLGSGTSVVK GPGFTGGDIL RRTSPGQIST LRVNITAPLS QRYRVRIRYA STTNLQFHTS 

       550        560        570        580        590        600 
IDGRPINQGN FSATMSSGSN LQSGSFRTVG FTTPFNFSNG SSVFTLSAHV FNSGNEVYID 

       610        620        630        640        650        660 
RIEFVPAEVT FEAEYDLERA QKAVNELFTS SNQIGLKTDV TDYHIDQVSN LVECLSDEFC 

       670        680        690        700        710        720 
LDEKKELSEK VKHAKRLSDE RNLLQDPNFR GINRQLDRGW RGSTDITIQG GDDVFKENYV 

       730        740        750        760        770        780 
TLLGTFDECY PTYLYQKIDE SKLKAYTRYQ LRGYIEDSQD LEIYLIRYNA KHETVNVPGT 

       790        800        810        820        830        840 
GSLWPLSAPS PIGKCAHHSH HFSLDIDVGC TDLNEDLGVW VIFKIKTQDG HARLGNLEFL 

       850        860        870        880        890        900 
EEKPLVGEAL ARVKRAEKKW RDKREKLEWE TNIVYKEAKE SVDALFVNSQ YDRLQADTNI 

       910        920        930        940        950        960 
AMIHAADKRV HSIREAYLPE LSVIPGVNAA IFEELEGRIF TAFSLYDARN VIKNGDFNNG 

       970        980        990       1000       1010       1020 
LSCWNVKGHV DVEEQNNHRS VLVVPEWEAE VSQEVRVCPG RGYILRVTAY KEGYGEGCVT 

      1030       1040       1050       1060       1070       1080 
IHEIENNTDE LKFSNCVEEE VYPNNTVTCN DYTATQEEYE GTYTSRNRGY DGAYESNSSV 

      1090       1100       1110       1120       1130       1140 
PADYASAYEE KAYTDGRRDN PCESNRGYGD YTPLPAGYVT KELEYFPETD KVWIEIGETE 

      1150 
GTFIVDSVEL LLMEE 

« Hide

References

[1]"The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1."
Geiser M., Schweitzer S., Grimm C.
Gene 48:109-118(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HD-1-Dippel.
[2]"Structural similarity between the lepidoptera- and diptera-specific insecticidal endotoxin genes of Bacillus thuringiensis subsp. 'kurstaki' and 'israelensis'."
Thorne L., Garduno F., Thompson T., Decker D., Zounes M., Wild M., Walfield A.M., Pollock T.J.
J. Bacteriol. 166:801-811(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HD-1-Dippel.
[3]"Cloning and nucleotide sequencing of two insecticidal delta-endotoxin genes from Bacillus thuringiensis var. kurstaki HD-1 DNA."
Kondo S., Tamura N., Kunitate A., Hattori M., Akashi A., Ohmori I.
Agric. Biol. Chem. 51:455-463(1987) [AGRICOLA] [Europe PMC]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HD-1-Dippel.
[4]"Insect tolerant transgenic tomato plants."
Fischhoff D.A., Bowdish K.S., Perlak F.J., Marrone P.G., McCormick S.M., Niedermeyer J.G., Dean D.A., Kusano-Kretzmer K., Mayer E.J., Rochester D.E., Rogers S.G., Fraley R.T.
Biotechnology (N.Y.) 5:807-813(1987)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HD-1-Dippel.
[5]"Sequence of a lepidopteran toxin gene of Bacillus thuringiensis subsp kurstaki NRD-12."
Hefford M.A., Brousseau R., Prefontaine G., Hanna Z., Condie J.A., Lau P.C.K.
J. Biotechnol. 6:307-322(1987)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NRD-12.
[6]"Characterization of Bacillus thuringiensis subsp. kurstaki strain S93 effective against the Fall armyworm, Spodoptera frugiperda and cloning of a cry1Ab gene."
Silva-Werneck J.O., De-Souza M.T., Dias J.M.C.S., Ribeiro B.M.
Can. J. Microbiol. 45:464-471(1999)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S93.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M15271 Genomic DNA. Translation: AAA22561.1.
M12661 Genomic DNA. Translation: AAA22613.1.
D00117 Genomic DNA. Translation: BAA00071.1.
M37263 Genomic DNA. Translation: AAA22420.1.
AF059670 Genomic DNA. Translation: AAC64003.1.
A03793 Unassigned DNA. Translation: CAA00303.1.
A09398 Unassigned DNA. Translation: CAA00840.1.
PIRA29125.
A29838.
JD0002. A90025.
I39838.

3D structure databases

ProteinModelPortalP0A370.
SMRP0A370. Positions 33-610.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB1.C.2.1.3. the channel-forming -endotoxin insecticidal crystal protein (icp) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 1 hit.
InterProIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCR1AB_BACTK
AccessionPrimary (citable) accession number: P0A370
Secondary accession number(s): P06577 expand/collapse secondary AC list , P06578, P09663, P09666, P09667, P21257, Q45789
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 16, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families