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P0A370

- CR1AB_BACTK

UniProt

P0A370 - CR1AB_BACTK

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Protein
Pesticidal crystal protein cry1Ab
Gene
cry1Ab, bt2, cry-1-2, cry1A(b), cryIA(b), cryIC1
Organism
Bacillus thuringiensis subsp. kurstaki
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

GO - Biological processi

  1. defense response Source: InterPro
  2. pathogenesis Source: InterPro
  3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Sporulation

Protein family/group databases

TCDBi1.C.2.1.3. the channel-forming -endotoxin insecticidal crystal protein (icp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Pesticidal crystal protein cry1Ab
Alternative name(s):
130 kDa crystal protein
Crystaline entomocidal protoxin
Insecticidal delta-endotoxin CryIA(b)
Gene namesi
Name:cry1Ab
Synonyms:bt2, cry-1-2, cry1A(b), cryIA(b), cryIC1
Encoded oniPlasmid0 Publication
OrganismiBacillus thuringiensis subsp. kurstaki
Taxonomic identifieri29339 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Pathology & Biotechi

Biotechnological usei

Introduced by genetic manipulation and expressed in insect-resistant maize by Monsanto, Northrup King and Ciba Geigy.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11551155Pesticidal crystal protein cry1Ab
PRO_0000174023Add
BLAST

Expressioni

Developmental stagei

The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

Structurei

3D structure databases

ProteinModelPortaliP0A370.
SMRiP0A370. Positions 33-610.

Family & Domainsi

Sequence similaritiesi

Belongs to the delta endotoxin family.

Family and domain databases

Gene3Di1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A370-1 [UniParc]FASTAAdd to Basket

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MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF     50
VPGAGFVLGL VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL 100
EGLSNLYQIY AESFREWEAD PTNPALREEM RIQFNDMNSA LTTAIPLFAV 150
QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ RWGFDAATIN SRYNDLTRLI 200
GNYTDHAVRW YNTGLERVWG PDSRDWIRYN QFRRELTLTV LDIVSLFPNY 250
DSRTYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIEGS IRSPHLMDIL 300
NSITIYTDAH RGEYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI 350
VAQLGQGVYR TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV 400
YRKSGTVDSL DEIPPQNNNV PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA 450
PMFSWIHRSA EFNNIIPSSQ ITQIPLTKST NLGSGTSVVK GPGFTGGDIL 500
RRTSPGQIST LRVNITAPLS QRYRVRIRYA STTNLQFHTS IDGRPINQGN 550
FSATMSSGSN LQSGSFRTVG FTTPFNFSNG SSVFTLSAHV FNSGNEVYID 600
RIEFVPAEVT FEAEYDLERA QKAVNELFTS SNQIGLKTDV TDYHIDQVSN 650
LVECLSDEFC LDEKKELSEK VKHAKRLSDE RNLLQDPNFR GINRQLDRGW 700
RGSTDITIQG GDDVFKENYV TLLGTFDECY PTYLYQKIDE SKLKAYTRYQ 750
LRGYIEDSQD LEIYLIRYNA KHETVNVPGT GSLWPLSAPS PIGKCAHHSH 800
HFSLDIDVGC TDLNEDLGVW VIFKIKTQDG HARLGNLEFL EEKPLVGEAL 850
ARVKRAEKKW RDKREKLEWE TNIVYKEAKE SVDALFVNSQ YDRLQADTNI 900
AMIHAADKRV HSIREAYLPE LSVIPGVNAA IFEELEGRIF TAFSLYDARN 950
VIKNGDFNNG LSCWNVKGHV DVEEQNNHRS VLVVPEWEAE VSQEVRVCPG 1000
RGYILRVTAY KEGYGEGCVT IHEIENNTDE LKFSNCVEEE VYPNNTVTCN 1050
DYTATQEEYE GTYTSRNRGY DGAYESNSSV PADYASAYEE KAYTDGRRDN 1100
PCESNRGYGD YTPLPAGYVT KELEYFPETD KVWIEIGETE GTFIVDSVEL 1150
LLMEE 1155
Length:1,155
Mass (Da):130,624
Last modified:January 1, 1988 - v1
Checksum:i43461A64C7AC7CAF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti207 – 2071A → R in AAA22613. 1 Publication
Sequence conflicti382 – 40625VLDGT…RKSGT → GPDGGRICLWNLLKFGQPPY TEKAEP in AAA22613. 1 Publication
Add
BLAST
Sequence conflicti410 – 4101L → P in AAA22613. 1 Publication
Sequence conflicti430 – 4323LSH → CLAY in AAA22613. 1 Publication
Sequence conflicti437 – 4371R → Y in AAA22613. 1 Publication
Sequence conflicti447 – 4471I → V in AAA22613. 1 Publication
Sequence conflicti452 – 4587MFSWIHR → NDSSWTYC in AAA22613. 1 Publication
Sequence conflicti461 – 4611E → N in AAA22420. 1 Publication
Sequence conflicti463 – 4653NNI → GDV in AAA22613. 1 Publication
Sequence conflicti479 – 4868STNLGSGT → LQSWLWN in AAA22613. 1 Publication
Sequence conflicti492 – 4921P → L in AAA22613. 1 Publication
Sequence conflicti501 – 5055RRTSP → EELT in AAA22613. 1 Publication
Sequence conflicti542 – 5421D → H in AAA22420. 1 Publication
Sequence conflicti568 – 5692TV → HL in AAA22420. 1 Publication
Sequence conflicti665 – 6651K → E in AAA22420. 1 Publication
Sequence conflicti675 – 6762KR → NG in AAA22420. 1 Publication
Sequence conflicti703 – 7031S → N in AAA22613. 1 Publication
Sequence conflicti712 – 7121D → H in AAA22613. 1 Publication
Sequence conflicti832 – 8321A → E1 Publication
Sequence conflicti836 – 8361N → I in AAA22420. 1 Publication
Sequence conflicti842 – 8432EK → GRA1 Publication
Sequence conflicti1016 – 10161E → T in AAA22420. 1 Publication
Sequence conflicti1060 – 10601E → G in AAA22420. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15271 Genomic DNA. Translation: AAA22561.1.
M12661 Genomic DNA. Translation: AAA22613.1.
D00117 Genomic DNA. Translation: BAA00071.1.
M37263 Genomic DNA. Translation: AAA22420.1.
AF059670 Genomic DNA. Translation: AAC64003.1.
A03793 Unassigned DNA. Translation: CAA00303.1.
A09398 Unassigned DNA. Translation: CAA00840.1.
PIRiA29125.
A29838.
A90025. JD0002.
I39838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15271 Genomic DNA. Translation: AAA22561.1 .
M12661 Genomic DNA. Translation: AAA22613.1 .
D00117 Genomic DNA. Translation: BAA00071.1 .
M37263 Genomic DNA. Translation: AAA22420.1 .
AF059670 Genomic DNA. Translation: AAC64003.1 .
A03793 Unassigned DNA. Translation: CAA00303.1 .
A09398 Unassigned DNA. Translation: CAA00840.1 .
PIRi A29125.
A29838.
A90025. JD0002.
I39838.

3D structure databases

ProteinModelPortali P0A370.
SMRi P0A370. Positions 33-610.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

TCDBi 1.C.2.1.3. the channel-forming -endotoxin insecticidal crystal protein (icp) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 1 hit.
InterProi IPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view ]
Pfami PF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1."
    Geiser M., Schweitzer S., Grimm C.
    Gene 48:109-118(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-1-Dippel.
  2. "Structural similarity between the lepidoptera- and diptera-specific insecticidal endotoxin genes of Bacillus thuringiensis subsp. 'kurstaki' and 'israelensis'."
    Thorne L., Garduno F., Thompson T., Decker D., Zounes M., Wild M., Walfield A.M., Pollock T.J.
    J. Bacteriol. 166:801-811(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-1-Dippel.
  3. "Cloning and nucleotide sequencing of two insecticidal delta-endotoxin genes from Bacillus thuringiensis var. kurstaki HD-1 DNA."
    Kondo S., Tamura N., Kunitate A., Hattori M., Akashi A., Ohmori I.
    Agric. Biol. Chem. 51:455-463(1987)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-1-Dippel.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-1-Dippel.
  5. "Sequence of a lepidopteran toxin gene of Bacillus thuringiensis subsp kurstaki NRD-12."
    Hefford M.A., Brousseau R., Prefontaine G., Hanna Z., Condie J.A., Lau P.C.K.
    J. Biotechnol. 6:307-322(1987)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NRD-12.
  6. "Characterization of Bacillus thuringiensis subsp. kurstaki strain S93 effective against the Fall armyworm, Spodoptera frugiperda and cloning of a cry1Ab gene."
    Silva-Werneck J.O., De-Souza M.T., Dias J.M.C.S., Ribeiro B.M.
    Can. J. Microbiol. 45:464-471(1999)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S93.

Entry informationi

Entry nameiCR1AB_BACTK
AccessioniPrimary (citable) accession number: P0A370
Secondary accession number(s): P06577
, P06578, P09663, P09666, P09667, P21257, Q45789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: October 16, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Toxic segment of the protein is located in the N-terminus.

Keywords - Technical termi

Genetically modified food, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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