Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0A370

- CR1AB_BACTK

UniProt

P0A370 - CR1AB_BACTK

Protein

Pesticidal crystal protein cry1Ab

Gene

cry1Ab

Organism
Bacillus thuringiensis subsp. kurstaki
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

    GO - Biological processi

    1. defense response Source: InterPro
    2. pathogenesis Source: InterPro
    3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Toxin

    Keywords - Biological processi

    Sporulation

    Protein family/group databases

    TCDBi1.C.2.1.3. the channel-forming -endotoxin insecticidal crystal protein (icp) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pesticidal crystal protein cry1Ab
    Alternative name(s):
    130 kDa crystal protein
    Crystaline entomocidal protoxin
    Insecticidal delta-endotoxin CryIA(b)
    Gene namesi
    Name:cry1Ab
    Synonyms:bt2, cry-1-2, cry1A(b), cryIA(b), cryIC1
    Encoded oniPlasmid0 Publication
    OrganismiBacillus thuringiensis subsp. kurstaki
    Taxonomic identifieri29339 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

    Pathology & Biotechi

    Biotechnological usei

    Introduced by genetic manipulation and expressed in insect-resistant maize by Monsanto, Northrup King and Ciba Geigy.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11551155Pesticidal crystal protein cry1AbPRO_0000174023Add
    BLAST

    Expressioni

    Developmental stagei

    The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A370.
    SMRiP0A370. Positions 33-610.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the delta endotoxin family.Curated

    Family and domain databases

    Gene3Di1.20.190.10. 1 hit.
    2.100.10.10. 1 hit.
    2.60.120.260. 1 hit.
    InterProiIPR005638. Endotoxin_C.
    IPR001178. Endotoxin_cen_dom.
    IPR015790. Endotoxin_cen_dom_subgr1.
    IPR005639. Endotoxin_N.
    IPR008979. Galactose-bd-like.
    [Graphical view]
    PfamiPF03944. Endotoxin_C. 1 hit.
    PF00555. Endotoxin_M. 1 hit.
    PF03945. Endotoxin_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF51096. SSF51096. 1 hit.
    SSF56849. SSF56849. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A370-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF     50
    VPGAGFVLGL VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL 100
    EGLSNLYQIY AESFREWEAD PTNPALREEM RIQFNDMNSA LTTAIPLFAV 150
    QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ RWGFDAATIN SRYNDLTRLI 200
    GNYTDHAVRW YNTGLERVWG PDSRDWIRYN QFRRELTLTV LDIVSLFPNY 250
    DSRTYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIEGS IRSPHLMDIL 300
    NSITIYTDAH RGEYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI 350
    VAQLGQGVYR TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV 400
    YRKSGTVDSL DEIPPQNNNV PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA 450
    PMFSWIHRSA EFNNIIPSSQ ITQIPLTKST NLGSGTSVVK GPGFTGGDIL 500
    RRTSPGQIST LRVNITAPLS QRYRVRIRYA STTNLQFHTS IDGRPINQGN 550
    FSATMSSGSN LQSGSFRTVG FTTPFNFSNG SSVFTLSAHV FNSGNEVYID 600
    RIEFVPAEVT FEAEYDLERA QKAVNELFTS SNQIGLKTDV TDYHIDQVSN 650
    LVECLSDEFC LDEKKELSEK VKHAKRLSDE RNLLQDPNFR GINRQLDRGW 700
    RGSTDITIQG GDDVFKENYV TLLGTFDECY PTYLYQKIDE SKLKAYTRYQ 750
    LRGYIEDSQD LEIYLIRYNA KHETVNVPGT GSLWPLSAPS PIGKCAHHSH 800
    HFSLDIDVGC TDLNEDLGVW VIFKIKTQDG HARLGNLEFL EEKPLVGEAL 850
    ARVKRAEKKW RDKREKLEWE TNIVYKEAKE SVDALFVNSQ YDRLQADTNI 900
    AMIHAADKRV HSIREAYLPE LSVIPGVNAA IFEELEGRIF TAFSLYDARN 950
    VIKNGDFNNG LSCWNVKGHV DVEEQNNHRS VLVVPEWEAE VSQEVRVCPG 1000
    RGYILRVTAY KEGYGEGCVT IHEIENNTDE LKFSNCVEEE VYPNNTVTCN 1050
    DYTATQEEYE GTYTSRNRGY DGAYESNSSV PADYASAYEE KAYTDGRRDN 1100
    PCESNRGYGD YTPLPAGYVT KELEYFPETD KVWIEIGETE GTFIVDSVEL 1150
    LLMEE 1155
    Length:1,155
    Mass (Da):130,624
    Last modified:January 1, 1988 - v1
    Checksum:i43461A64C7AC7CAF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti207 – 2071A → R in AAA22613. (PubMed:3011746)Curated
    Sequence conflicti382 – 40625VLDGT…RKSGT → GPDGGRICLWNLLKFGQPPY TEKAEP in AAA22613. (PubMed:3011746)CuratedAdd
    BLAST
    Sequence conflicti410 – 4101L → P in AAA22613. (PubMed:3011746)Curated
    Sequence conflicti430 – 4323LSH → CLAY in AAA22613. (PubMed:3011746)Curated
    Sequence conflicti437 – 4371R → Y in AAA22613. (PubMed:3011746)Curated
    Sequence conflicti447 – 4471I → V in AAA22613. (PubMed:3011746)Curated
    Sequence conflicti452 – 4587MFSWIHR → NDSSWTYC in AAA22613. (PubMed:3011746)Curated
    Sequence conflicti461 – 4611E → N in AAA22420. 1 PublicationCurated
    Sequence conflicti463 – 4653NNI → GDV in AAA22613. (PubMed:3011746)Curated
    Sequence conflicti479 – 4868STNLGSGT → LQSWLWN in AAA22613. (PubMed:3011746)Curated
    Sequence conflicti492 – 4921P → L in AAA22613. (PubMed:3011746)Curated
    Sequence conflicti501 – 5055RRTSP → EELT in AAA22613. (PubMed:3011746)Curated
    Sequence conflicti542 – 5421D → H in AAA22420. 1 PublicationCurated
    Sequence conflicti568 – 5692TV → HL in AAA22420. 1 PublicationCurated
    Sequence conflicti665 – 6651K → E in AAA22420. 1 PublicationCurated
    Sequence conflicti675 – 6762KR → NG in AAA22420. 1 PublicationCurated
    Sequence conflicti703 – 7031S → N in AAA22613. (PubMed:3011746)Curated
    Sequence conflicti712 – 7121D → H in AAA22613. (PubMed:3011746)Curated
    Sequence conflicti832 – 8321A → E1 PublicationCurated
    Sequence conflicti836 – 8361N → I in AAA22420. 1 PublicationCurated
    Sequence conflicti842 – 8432EK → GRA1 PublicationCurated
    Sequence conflicti1016 – 10161E → T in AAA22420. 1 PublicationCurated
    Sequence conflicti1060 – 10601E → G in AAA22420. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15271 Genomic DNA. Translation: AAA22561.1.
    M12661 Genomic DNA. Translation: AAA22613.1.
    D00117 Genomic DNA. Translation: BAA00071.1.
    M37263 Genomic DNA. Translation: AAA22420.1.
    AF059670 Genomic DNA. Translation: AAC64003.1.
    A03793 Unassigned DNA. Translation: CAA00303.1.
    A09398 Unassigned DNA. Translation: CAA00840.1.
    PIRiA29125.
    A29838.
    A90025. JD0002.
    I39838.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15271 Genomic DNA. Translation: AAA22561.1 .
    M12661 Genomic DNA. Translation: AAA22613.1 .
    D00117 Genomic DNA. Translation: BAA00071.1 .
    M37263 Genomic DNA. Translation: AAA22420.1 .
    AF059670 Genomic DNA. Translation: AAC64003.1 .
    A03793 Unassigned DNA. Translation: CAA00303.1 .
    A09398 Unassigned DNA. Translation: CAA00840.1 .
    PIRi A29125.
    A29838.
    A90025. JD0002.
    I39838.

    3D structure databases

    ProteinModelPortali P0A370.
    SMRi P0A370. Positions 33-610.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    TCDBi 1.C.2.1.3. the channel-forming -endotoxin insecticidal crystal protein (icp) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.20.190.10. 1 hit.
    2.100.10.10. 1 hit.
    2.60.120.260. 1 hit.
    InterProi IPR005638. Endotoxin_C.
    IPR001178. Endotoxin_cen_dom.
    IPR015790. Endotoxin_cen_dom_subgr1.
    IPR005639. Endotoxin_N.
    IPR008979. Galactose-bd-like.
    [Graphical view ]
    Pfami PF03944. Endotoxin_C. 1 hit.
    PF00555. Endotoxin_M. 1 hit.
    PF03945. Endotoxin_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF51096. SSF51096. 1 hit.
    SSF56849. SSF56849. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1."
      Geiser M., Schweitzer S., Grimm C.
      Gene 48:109-118(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HD-1-Dippel.
    2. "Structural similarity between the lepidoptera- and diptera-specific insecticidal endotoxin genes of Bacillus thuringiensis subsp. 'kurstaki' and 'israelensis'."
      Thorne L., Garduno F., Thompson T., Decker D., Zounes M., Wild M., Walfield A.M., Pollock T.J.
      J. Bacteriol. 166:801-811(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HD-1-Dippel.
    3. "Cloning and nucleotide sequencing of two insecticidal delta-endotoxin genes from Bacillus thuringiensis var. kurstaki HD-1 DNA."
      Kondo S., Tamura N., Kunitate A., Hattori M., Akashi A., Ohmori I.
      Agric. Biol. Chem. 51:455-463(1987)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HD-1-Dippel.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HD-1-Dippel.
    5. "Sequence of a lepidopteran toxin gene of Bacillus thuringiensis subsp kurstaki NRD-12."
      Hefford M.A., Brousseau R., Prefontaine G., Hanna Z., Condie J.A., Lau P.C.K.
      J. Biotechnol. 6:307-322(1987)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: NRD-12.
    6. "Characterization of Bacillus thuringiensis subsp. kurstaki strain S93 effective against the Fall armyworm, Spodoptera frugiperda and cloning of a cry1Ab gene."
      Silva-Werneck J.O., De-Souza M.T., Dias J.M.C.S., Ribeiro B.M.
      Can. J. Microbiol. 45:464-471(1999)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S93.

    Entry informationi

    Entry nameiCR1AB_BACTK
    AccessioniPrimary (citable) accession number: P0A370
    Secondary accession number(s): P06577
    , P06578, P09663, P09666, P09667, P21257, Q45789
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Toxic segment of the protein is located in the N-terminus.

    Keywords - Technical termi

    Genetically modified food, Plasmid

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3