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Protein

Pesticidal crystal protein Cry1Ab

Gene

cry1Ab

Organism
Bacillus thuringiensis subsp. kurstaki
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Sporulation

Protein family/group databases

TCDBi1.C.2.1.3. the channel-forming -endotoxin insecticidal crystal protein (icp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Pesticidal crystal protein Cry1Ab
Alternative name(s):
130 kDa crystal protein
Crystaline entomocidal protoxin
Insecticidal delta-endotoxin CryIA(b)
Gene namesi
Name:cry1Ab
Synonyms:bt2, cry-1-2, cry1A(b), cryIA(b), cryIC1
Encoded oniPlasmid pUCBtS931 Publication
OrganismiBacillus thuringiensis subsp. kurstaki
Taxonomic identifieri29339 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Pathology & Biotechi

Biotechnological usei

Introduced by genetic manipulation and expressed in insect-resistant maize by Monsanto, Northrup King and Ciba Geigy.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001740231 – 1155Pesticidal crystal protein Cry1AbAdd BLAST1155

Expressioni

Developmental stagei

The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

Structurei

3D structure databases

ProteinModelPortaliP0A370.
SMRiP0A370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the delta endotoxin family.Curated

Family and domain databases

Gene3Di1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 2 hits.
InterProiIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF
60 70 80 90 100
VPGAGFVLGL VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL
110 120 130 140 150
EGLSNLYQIY AESFREWEAD PTNPALREEM RIQFNDMNSA LTTAIPLFAV
160 170 180 190 200
QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ RWGFDAATIN SRYNDLTRLI
210 220 230 240 250
GNYTDHAVRW YNTGLERVWG PDSRDWIRYN QFRRELTLTV LDIVSLFPNY
260 270 280 290 300
DSRTYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIEGS IRSPHLMDIL
310 320 330 340 350
NSITIYTDAH RGEYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI
360 370 380 390 400
VAQLGQGVYR TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV
410 420 430 440 450
YRKSGTVDSL DEIPPQNNNV PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA
460 470 480 490 500
PMFSWIHRSA EFNNIIPSSQ ITQIPLTKST NLGSGTSVVK GPGFTGGDIL
510 520 530 540 550
RRTSPGQIST LRVNITAPLS QRYRVRIRYA STTNLQFHTS IDGRPINQGN
560 570 580 590 600
FSATMSSGSN LQSGSFRTVG FTTPFNFSNG SSVFTLSAHV FNSGNEVYID
610 620 630 640 650
RIEFVPAEVT FEAEYDLERA QKAVNELFTS SNQIGLKTDV TDYHIDQVSN
660 670 680 690 700
LVECLSDEFC LDEKKELSEK VKHAKRLSDE RNLLQDPNFR GINRQLDRGW
710 720 730 740 750
RGSTDITIQG GDDVFKENYV TLLGTFDECY PTYLYQKIDE SKLKAYTRYQ
760 770 780 790 800
LRGYIEDSQD LEIYLIRYNA KHETVNVPGT GSLWPLSAPS PIGKCAHHSH
810 820 830 840 850
HFSLDIDVGC TDLNEDLGVW VIFKIKTQDG HARLGNLEFL EEKPLVGEAL
860 870 880 890 900
ARVKRAEKKW RDKREKLEWE TNIVYKEAKE SVDALFVNSQ YDRLQADTNI
910 920 930 940 950
AMIHAADKRV HSIREAYLPE LSVIPGVNAA IFEELEGRIF TAFSLYDARN
960 970 980 990 1000
VIKNGDFNNG LSCWNVKGHV DVEEQNNHRS VLVVPEWEAE VSQEVRVCPG
1010 1020 1030 1040 1050
RGYILRVTAY KEGYGEGCVT IHEIENNTDE LKFSNCVEEE VYPNNTVTCN
1060 1070 1080 1090 1100
DYTATQEEYE GTYTSRNRGY DGAYESNSSV PADYASAYEE KAYTDGRRDN
1110 1120 1130 1140 1150
PCESNRGYGD YTPLPAGYVT KELEYFPETD KVWIEIGETE GTFIVDSVEL

LLMEE
Length:1,155
Mass (Da):130,624
Last modified:January 1, 1988 - v1
Checksum:i43461A64C7AC7CAF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207A → R in AAA22613 (PubMed:3011746).Curated1
Sequence conflicti382 – 406VLDGT…RKSGT → GPDGGRICLWNLLKFGQPPY TEKAEP in AAA22613 (PubMed:3011746).CuratedAdd BLAST25
Sequence conflicti410L → P in AAA22613 (PubMed:3011746).Curated1
Sequence conflicti430 – 432LSH → CLAY in AAA22613 (PubMed:3011746).Curated3
Sequence conflicti437R → Y in AAA22613 (PubMed:3011746).Curated1
Sequence conflicti447I → V in AAA22613 (PubMed:3011746).Curated1
Sequence conflicti452 – 458MFSWIHR → NDSSWTYC in AAA22613 (PubMed:3011746).Curated7
Sequence conflicti461E → N in AAA22420 (Ref. 5) Curated1
Sequence conflicti463 – 465NNI → GDV in AAA22613 (PubMed:3011746).Curated3
Sequence conflicti479 – 486STNLGSGT → LQSWLWN in AAA22613 (PubMed:3011746).Curated8
Sequence conflicti492P → L in AAA22613 (PubMed:3011746).Curated1
Sequence conflicti501 – 505RRTSP → EELT in AAA22613 (PubMed:3011746).Curated5
Sequence conflicti542D → H in AAA22420 (Ref. 5) Curated1
Sequence conflicti568 – 569TV → HL in AAA22420 (Ref. 5) Curated2
Sequence conflicti665K → E in AAA22420 (Ref. 5) Curated1
Sequence conflicti675 – 676KR → NG in AAA22420 (Ref. 5) Curated2
Sequence conflicti703S → N in AAA22613 (PubMed:3011746).Curated1
Sequence conflicti712D → H in AAA22613 (PubMed:3011746).Curated1
Sequence conflicti832A → E no nucleotide entry (Ref. 4) Curated1
Sequence conflicti836N → I in AAA22420 (Ref. 5) Curated1
Sequence conflicti842 – 843EK → GRA no nucleotide entry (Ref. 4) Curated2
Sequence conflicti1016E → T in AAA22420 (Ref. 5) Curated1
Sequence conflicti1060E → G in AAA22420 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15271 Genomic DNA. Translation: AAA22561.1.
M12661 Genomic DNA. Translation: AAA22613.1.
D00117 Genomic DNA. Translation: BAA00071.1.
M37263 Genomic DNA. Translation: AAA22420.1.
AF059670 Genomic DNA. Translation: AAC64003.1.
A03793 Unassigned DNA. Translation: CAA00303.1.
A09398 Unassigned DNA. Translation: CAA00840.1.
PIRiA29125.
A29838.
A90025. JD0002.
I39838.
RefSeqiWP_000369819.1. NZ_CP010003.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15271 Genomic DNA. Translation: AAA22561.1.
M12661 Genomic DNA. Translation: AAA22613.1.
D00117 Genomic DNA. Translation: BAA00071.1.
M37263 Genomic DNA. Translation: AAA22420.1.
AF059670 Genomic DNA. Translation: AAC64003.1.
A03793 Unassigned DNA. Translation: CAA00303.1.
A09398 Unassigned DNA. Translation: CAA00840.1.
PIRiA29125.
A29838.
A90025. JD0002.
I39838.
RefSeqiWP_000369819.1. NZ_CP010003.1.

3D structure databases

ProteinModelPortaliP0A370.
SMRiP0A370.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.2.1.3. the channel-forming -endotoxin insecticidal crystal protein (icp) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 2 hits.
InterProiIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCR1AB_BACTK
AccessioniPrimary (citable) accession number: P0A370
Secondary accession number(s): P06577
, P06578, P09663, P09666, P09667, P21257, Q45789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 30, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Toxic segment of the protein is located in the N-terminus.
In strain S93 this is encoded on plasmid pUCBtS39.1 Publication

Keywords - Technical termi

Genetically modified food, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.