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Protein

Pesticidal crystal protein Cry1Aa

Gene

cry1Aa

Organism
Bacillus thuringiensis subsp. kurstaki
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Sporulation

Names & Taxonomyi

Protein namesi
Recommended name:
Pesticidal crystal protein Cry1Aa
Alternative name(s):
133 kDa crystal protein
Crystaline entomocidal protoxin
Insecticidal delta-endotoxin CryIA(a)
Gene namesi
Name:cry1Aa
Synonyms:cry-1-1, cry1A(a), cryA, crybns3-1, cryIA(a), icp
OrganismiBacillus thuringiensis subsp. kurstaki
Taxonomic identifieri29339 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001740191 – 1176Pesticidal crystal protein Cry1AaAdd BLAST1176

Expressioni

Developmental stagei

The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
btr175Q9XY0910EBI-7210432,EBI-7210462From a different organism.

Protein-protein interaction databases

IntActiP0A366. 1 interactor.
MINTiMINT-7259645.

Structurei

Secondary structure

11176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 48Combined sources14
Beta strandi51 – 53Combined sources3
Helixi54 – 63Combined sources10
Helixi70 – 84Combined sources15
Helixi90 – 119Combined sources30
Helixi124 – 144Combined sources21
Helixi145 – 148Combined sources4
Helixi154 – 178Combined sources25
Helixi180 – 182Combined sources3
Helixi186 – 218Combined sources33
Helixi223 – 239Combined sources17
Helixi241 – 244Combined sources4
Helixi245 – 250Combined sources6
Turni252 – 254Combined sources3
Beta strandi266 – 269Combined sources4
Helixi271 – 274Combined sources4
Helixi284 – 289Combined sources6
Beta strandi298 – 310Combined sources13
Beta strandi313 – 325Combined sources13
Helixi326 – 328Combined sources3
Beta strandi344 – 351Combined sources8
Beta strandi357 – 367Combined sources11
Beta strandi380 – 390Combined sources11
Beta strandi393 – 395Combined sources3
Beta strandi400 – 403Combined sources4
Beta strandi407 – 409Combined sources3
Helixi410 – 412Combined sources3
Helixi423 – 426Combined sources4
Beta strandi429 – 434Combined sources6
Beta strandi444 – 449Combined sources6
Beta strandi452 – 456Combined sources5
Beta strandi467 – 474Combined sources8
Helixi475 – 477Combined sources3
Beta strandi479 – 481Combined sources3
Beta strandi486 – 488Combined sources3
Beta strandi492 – 496Combined sources5
Beta strandi498 – 514Combined sources17
Beta strandi522 – 532Combined sources11
Beta strandi534 – 540Combined sources7
Beta strandi543 – 550Combined sources8
Helixi562 – 564Combined sources3
Beta strandi566 – 569Combined sources4
Beta strandi577 – 590Combined sources14
Beta strandi596 – 605Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CIYX-ray2.25A29-618[»]
ProteinModelPortaliP0A366.
SMRiP0A366.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A366.

Family & Domainsi

Sequence similaritiesi

Belongs to the delta endotoxin family.Curated

Family and domain databases

Gene3Di1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 2 hits.
InterProiIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A366-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF
60 70 80 90 100
VPGAGFVLGL VDIIWGIFGP SQWDAFPVQI EQLINQRIEE FARNQAISRL
110 120 130 140 150
EGLSNLYQIY AESFREWEAD PTNPALREEM RIQFNDMNSA LTTAIPLLAV
160 170 180 190 200
QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ RWGFDAATIN SRYNDLTRLI
210 220 230 240 250
GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV LDIVALFSNY
260 270 280 290 300
DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGMAQRIEQN IRQPHLMDIL
310 320 330 340 350
NSITIYTDVH RGFNYWSGHQ ITASPVGFSG PEFAFPLFGN AGNAAPPVLV
360 370 380 390 400
SLTGLGIFRT LSSPLYRRII LGSGPNNQEL FVLDGTEFSF ASLTTNLPST
410 420 430 440 450
IYRQRGTVDS LDVIPPQDNS VPPRAGFSHR LSHVTMLSQA AGAVYTLRAP
460 470 480 490 500
TFSWQHRSAE FNNIIPSSQI TQIPLTKSTN LGSGTSVVKG PGFTGGDILR
510 520 530 540 550
RTSPGQISTL RVNITAPLSQ RYRVRIRYAS TTNLQFHTSI DGRPINQGNF
560 570 580 590 600
SATMSSGSNL QSGSFRTVGF TTPFNFSNGS SVFTLSAHVF NSGNEVYIDR
610 620 630 640 650
IEFVPAEVTF EAEYDLERAQ KAVNELFTSS NQIGLKTDVT DYHIDQVSNL
660 670 680 690 700
VECLSDEFCL DEKQELSEKV KHAKRLSDER NLLQDPNFRG INRQLDRGWR
710 720 730 740 750
GSTDITIQGG DDVFKENYVT LLGTFDECYP TYLYQKIDES KLKAYTRYQL
760 770 780 790 800
RGYIEDSQDL EIYLIRYNAK HETVNVPGTG SLWPLSAQSP IGKCGEPNRC
810 820 830 840 850
APHLEWNPDL DCSCRDGEKC AHHSHHFSLD IDVGCTDLNE DLGVWVIFKI
860 870 880 890 900
KTQDGHARLG NLEFLEEKPL VGEALARVKR AEKKWRDKRE KLEWETNIVY
910 920 930 940 950
KEAKESVDAL FVNSQYDQLQ ADTNIAMIHA ADKRVHSIRE AYLPELSVIP
960 970 980 990 1000
GVNAAIFEEL EGRIFTAFSL YDARNVIKNG DFNNGLSCWN VKGHVDVEEQ
1010 1020 1030 1040 1050
NNQRSVLVVP EWEAEVSQEV RVCPGRGYIL RVTAYKEGYG EGCVTIHEIE
1060 1070 1080 1090 1100
NNTDELKFSN CVEEEIYPNN TVTCNDYTVN QEEYGGAYTS RNRGYNEAPS
1110 1120 1130 1140 1150
VPADYASVYE EKSYTDGRRE NPCEFNRGYR DYTPLPVGYV TKELEYFPET
1160 1170
DKVWIEIGET EGTFIVDSVE LLLMEE
Length:1,176
Mass (Da):133,120
Last modified:March 15, 2005 - v1
Checksum:iE2EE15AF12E5DD85
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1009V → L in AAA22353 (PubMed:2581950).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti77P → L in strain: BNS3. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11250 Genomic DNA. Translation: AAA22353.1.
Y09663 mRNA. Translation: CAA70856.1.
J01554 Genomic DNA. No translation available.
PIRiA22617.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11250 Genomic DNA. Translation: AAA22353.1.
Y09663 mRNA. Translation: CAA70856.1.
J01554 Genomic DNA. No translation available.
PIRiA22617.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CIYX-ray2.25A29-618[»]
ProteinModelPortaliP0A366.
SMRiP0A366.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0A366. 1 interactor.
MINTiMINT-7259645.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP0A366.

Family and domain databases

Gene3Di1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 2 hits.
InterProiIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCR1AA_BACTK
AccessioniPrimary (citable) accession number: P0A366
Secondary accession number(s): P02965
, P09664, P09665, P16478, Q9RED5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 15, 2005
Last modified: November 30, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Toxic segment of the protein is located in the N-terminus.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.