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P0A366

- CR1AA_BACTK

UniProt

P0A366 - CR1AA_BACTK

Protein

Pesticidal crystal protein cry1Aa

Gene

cry1Aa

Organism
Bacillus thuringiensis subsp. kurstaki
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. defense response Source: InterPro
    2. pathogenesis Source: InterPro
    3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Toxin

    Keywords - Biological processi

    Sporulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pesticidal crystal protein cry1Aa
    Alternative name(s):
    133 kDa crystal protein
    Crystaline entomocidal protoxin
    Insecticidal delta-endotoxin CryIA(a)
    Gene namesi
    Name:cry1Aa
    Synonyms:cry-1-1, cry1A(a), cryA, crybns3-1, cryIA(a), icp
    OrganismiBacillus thuringiensis subsp. kurstaki
    Taxonomic identifieri29339 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11761176Pesticidal crystal protein cry1AaPRO_0000174019Add
    BLAST

    Expressioni

    Developmental stagei

    The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    btr175Q9XY0910EBI-7210432,EBI-7210462From a different organism.

    Protein-protein interaction databases

    IntActiP0A366. 1 interaction.
    MINTiMINT-7259645.

    Structurei

    Secondary structure

    1
    1176
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi35 – 4814
    Beta strandi51 – 533
    Helixi54 – 6310
    Helixi70 – 8415
    Helixi90 – 11930
    Helixi124 – 14421
    Helixi145 – 1484
    Helixi154 – 17825
    Helixi180 – 1823
    Helixi186 – 21833
    Helixi223 – 23917
    Helixi241 – 2444
    Helixi245 – 2506
    Turni252 – 2543
    Beta strandi266 – 2694
    Helixi271 – 2744
    Helixi284 – 2896
    Beta strandi298 – 31013
    Beta strandi313 – 32513
    Helixi326 – 3283
    Beta strandi344 – 3518
    Beta strandi357 – 36711
    Beta strandi380 – 39011
    Beta strandi393 – 3953
    Beta strandi400 – 4034
    Beta strandi407 – 4093
    Helixi410 – 4123
    Helixi423 – 4264
    Beta strandi429 – 4346
    Beta strandi444 – 4496
    Beta strandi452 – 4565
    Beta strandi467 – 4748
    Helixi475 – 4773
    Beta strandi479 – 4813
    Beta strandi486 – 4883
    Beta strandi492 – 4965
    Beta strandi498 – 51417
    Beta strandi522 – 53211
    Beta strandi534 – 5407
    Beta strandi543 – 5508
    Helixi562 – 5643
    Beta strandi566 – 5694
    Beta strandi577 – 59014
    Beta strandi596 – 60510

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CIYX-ray2.25A29-618[»]
    ProteinModelPortaliP0A366.
    SMRiP0A366. Positions 33-609.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A366.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the delta endotoxin family.Curated

    Family and domain databases

    Gene3Di1.20.190.10. 1 hit.
    2.100.10.10. 1 hit.
    2.60.120.260. 1 hit.
    InterProiIPR005638. Endotoxin_C.
    IPR001178. Endotoxin_cen_dom.
    IPR015790. Endotoxin_cen_dom_subgr1.
    IPR005639. Endotoxin_N.
    IPR008979. Galactose-bd-like.
    [Graphical view]
    PfamiPF03944. Endotoxin_C. 1 hit.
    PF00555. Endotoxin_M. 1 hit.
    PF03945. Endotoxin_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF51096. SSF51096. 1 hit.
    SSF56849. SSF56849. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A366-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF     50
    VPGAGFVLGL VDIIWGIFGP SQWDAFPVQI EQLINQRIEE FARNQAISRL 100
    EGLSNLYQIY AESFREWEAD PTNPALREEM RIQFNDMNSA LTTAIPLLAV 150
    QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ RWGFDAATIN SRYNDLTRLI 200
    GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV LDIVALFSNY 250
    DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGMAQRIEQN IRQPHLMDIL 300
    NSITIYTDVH RGFNYWSGHQ ITASPVGFSG PEFAFPLFGN AGNAAPPVLV 350
    SLTGLGIFRT LSSPLYRRII LGSGPNNQEL FVLDGTEFSF ASLTTNLPST 400
    IYRQRGTVDS LDVIPPQDNS VPPRAGFSHR LSHVTMLSQA AGAVYTLRAP 450
    TFSWQHRSAE FNNIIPSSQI TQIPLTKSTN LGSGTSVVKG PGFTGGDILR 500
    RTSPGQISTL RVNITAPLSQ RYRVRIRYAS TTNLQFHTSI DGRPINQGNF 550
    SATMSSGSNL QSGSFRTVGF TTPFNFSNGS SVFTLSAHVF NSGNEVYIDR 600
    IEFVPAEVTF EAEYDLERAQ KAVNELFTSS NQIGLKTDVT DYHIDQVSNL 650
    VECLSDEFCL DEKQELSEKV KHAKRLSDER NLLQDPNFRG INRQLDRGWR 700
    GSTDITIQGG DDVFKENYVT LLGTFDECYP TYLYQKIDES KLKAYTRYQL 750
    RGYIEDSQDL EIYLIRYNAK HETVNVPGTG SLWPLSAQSP IGKCGEPNRC 800
    APHLEWNPDL DCSCRDGEKC AHHSHHFSLD IDVGCTDLNE DLGVWVIFKI 850
    KTQDGHARLG NLEFLEEKPL VGEALARVKR AEKKWRDKRE KLEWETNIVY 900
    KEAKESVDAL FVNSQYDQLQ ADTNIAMIHA ADKRVHSIRE AYLPELSVIP 950
    GVNAAIFEEL EGRIFTAFSL YDARNVIKNG DFNNGLSCWN VKGHVDVEEQ 1000
    NNQRSVLVVP EWEAEVSQEV RVCPGRGYIL RVTAYKEGYG EGCVTIHEIE 1050
    NNTDELKFSN CVEEEIYPNN TVTCNDYTVN QEEYGGAYTS RNRGYNEAPS 1100
    VPADYASVYE EKSYTDGRRE NPCEFNRGYR DYTPLPVGYV TKELEYFPET 1150
    DKVWIEIGET EGTFIVDSVE LLLMEE 1176
    Length:1,176
    Mass (Da):133,120
    Last modified:March 15, 2005 - v1
    Checksum:iE2EE15AF12E5DD85
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1009 – 10091V → L in AAA22353. (PubMed:2581950)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti77 – 771P → L in strain: BNS3.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11250 Genomic DNA. Translation: AAA22353.1.
    Y09663 mRNA. Translation: CAA70856.1.
    J01554 Genomic DNA. No translation available.
    PIRiA22617.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11250 Genomic DNA. Translation: AAA22353.1 .
    Y09663 mRNA. Translation: CAA70856.1 .
    J01554 Genomic DNA. No translation available.
    PIRi A22617.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CIY X-ray 2.25 A 29-618 [» ]
    ProteinModelPortali P0A366.
    SMRi P0A366. Positions 33-609.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0A366. 1 interaction.
    MINTi MINT-7259645.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P0A366.

    Family and domain databases

    Gene3Di 1.20.190.10. 1 hit.
    2.100.10.10. 1 hit.
    2.60.120.260. 1 hit.
    InterProi IPR005638. Endotoxin_C.
    IPR001178. Endotoxin_cen_dom.
    IPR015790. Endotoxin_cen_dom_subgr1.
    IPR005639. Endotoxin_N.
    IPR008979. Galactose-bd-like.
    [Graphical view ]
    Pfami PF03944. Endotoxin_C. 1 hit.
    PF00555. Endotoxin_M. 1 hit.
    PF03945. Endotoxin_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF51096. SSF51096. 1 hit.
    SSF56849. SSF56849. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The amino acid sequence of a crystal protein from Bacillus thuringiensis deduced from the DNA base sequence."
      Schnepf H.E., Wong H.C., Whiteley H.R.
      J. Biol. Chem. 260:6264-6272(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HD-1-Dippel.
    2. "Cloning and nucleotide sequence of a novel cry1Aa-type gene from Bacillus thuringiensis subsp.kurstaki."
      Tounsi S., J'Mal A., Zouari N., Jaoua S.
      Biotechnol. Lett. 21:771-775(1999)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BNS3.
    3. "Transcriptional and translational start sites for the Bacillus thuringiensis crystal protein gene."
      Wong H.C., Schnepf H.E., Whiteley H.R.
      J. Biol. Chem. 258:1960-1967(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-333, PROTEIN SEQUENCE OF 1-9.
      Strain: HD-1-Dippel.
    4. "Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation."
      Grochulski P., Masson L., Borisova S., Pusztai-Carey M., Schwartz J.L., Brousseau R., Cygler M.
      J. Mol. Biol. 254:447-464(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 33-609.
      Strain: HD-1-Dippel.

    Entry informationi

    Entry nameiCR1AA_BACTK
    AccessioniPrimary (citable) accession number: P0A366
    Secondary accession number(s): P02965
    , P09664, P09665, P16478, Q9RED5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Toxic segment of the protein is located in the N-terminus.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3