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Reviewed, UniProtKB/Swiss-Prot P0A366 (CR1AA_BACTK)

Last modified June 16, 2009. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Pesticidal crystal protein cry1Aa
Alternative name(s):
    Insecticidal delta-endotoxin CryIA(a)
    Crystaline entomocidal protoxin
    133 kDa crystal protein
Gene names
Name: cry1Aa
Synonyms: cry-1-1, cry1A(a), cryA, crybns3-1, cryIA(a), icp
OrganismBacillus thuringiensis subsp. kurstaki
Taxonomic identifier29339 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

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Protein attributes

Sequence length1176 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

Developmental stage

The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

Miscellaneous

Toxic segment of the protein is located in the N-terminus.

Sequence similarities

Belongs to the delta endotoxin family.

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Ontologies

Keywords
   Biological processSporulation
   Molecular functionToxin
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological processdefense response

Inferred from electronic annotation. Source: InterPro

pathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

sporulation resulting in formation of a cellular spore

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionreceptor binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11761176Pesticidal crystal protein cry1Aa
PRO_0000174019

Natural variations

Natural variant771P → L in strain: BNS3.

Experimental info

Sequence conflict10091V → L in AAA22353. Ref.1

Secondary structure

1 ............................................................................................. 1176
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P0A366-1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: E2EE15AF12E5DD85

FASTA1,176133,120
        10         20         30         40         50         60 
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL 

        70         80         90        100        110        120 
VDIIWGIFGP SQWDAFPVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD 

       130        140        150        160        170        180 
PTNPALREEM RIQFNDMNSA LTTAIPLLAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ 

       190        200        210        220        230        240 
RWGFDAATIN SRYNDLTRLI GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV 

       250        260        270        280        290        300 
LDIVALFSNY DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGMAQRIEQN IRQPHLMDIL 

       310        320        330        340        350        360 
NSITIYTDVH RGFNYWSGHQ ITASPVGFSG PEFAFPLFGN AGNAAPPVLV SLTGLGIFRT 

       370        380        390        400        410        420 
LSSPLYRRII LGSGPNNQEL FVLDGTEFSF ASLTTNLPST IYRQRGTVDS LDVIPPQDNS 

       430        440        450        460        470        480 
VPPRAGFSHR LSHVTMLSQA AGAVYTLRAP TFSWQHRSAE FNNIIPSSQI TQIPLTKSTN 

       490        500        510        520        530        540 
LGSGTSVVKG PGFTGGDILR RTSPGQISTL RVNITAPLSQ RYRVRIRYAS TTNLQFHTSI 

       550        560        570        580        590        600 
DGRPINQGNF SATMSSGSNL QSGSFRTVGF TTPFNFSNGS SVFTLSAHVF NSGNEVYIDR 

       610        620        630        640        650        660 
IEFVPAEVTF EAEYDLERAQ KAVNELFTSS NQIGLKTDVT DYHIDQVSNL VECLSDEFCL 

       670        680        690        700        710        720 
DEKQELSEKV KHAKRLSDER NLLQDPNFRG INRQLDRGWR GSTDITIQGG DDVFKENYVT 

       730        740        750        760        770        780 
LLGTFDECYP TYLYQKIDES KLKAYTRYQL RGYIEDSQDL EIYLIRYNAK HETVNVPGTG 

       790        800        810        820        830        840 
SLWPLSAQSP IGKCGEPNRC APHLEWNPDL DCSCRDGEKC AHHSHHFSLD IDVGCTDLNE 

       850        860        870        880        890        900 
DLGVWVIFKI KTQDGHARLG NLEFLEEKPL VGEALARVKR AEKKWRDKRE KLEWETNIVY 

       910        920        930        940        950        960 
KEAKESVDAL FVNSQYDQLQ ADTNIAMIHA ADKRVHSIRE AYLPELSVIP GVNAAIFEEL 

       970        980        990       1000       1010       1020 
EGRIFTAFSL YDARNVIKNG DFNNGLSCWN VKGHVDVEEQ NNQRSVLVVP EWEAEVSQEV 

      1030       1040       1050       1060       1070       1080 
RVCPGRGYIL RVTAYKEGYG EGCVTIHEIE NNTDELKFSN CVEEEIYPNN TVTCNDYTVN 

      1090       1100       1110       1120       1130       1140 
QEEYGGAYTS RNRGYNEAPS VPADYASVYE EKSYTDGRRE NPCEFNRGYR DYTPLPVGYV 

      1150       1160       1170 
TKELEYFPET DKVWIEIGET EGTFIVDSVE LLLMEE

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References

[1]"The amino acid sequence of a crystal protein from Bacillus thuringiensis deduced from the DNA base sequence."
Schnepf H.E., Wong H.C., Whiteley H.R.
J. Biol. Chem. 260:6264-6272(1985) [PubMed: 2581950] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HD-1-Dippel.
[2]"Cloning and nucleotide sequence of a novel cry1Aa-type gene from Bacillus thuringiensis subsp.kurstaki."
Tounsi S., J'Mal A., Zouari N., Jaoua S.
Biotechnol. Lett. 21:771-775(1999)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BNS3.
[3]"Transcriptional and translational start sites for the Bacillus thuringiensis crystal protein gene."
Wong H.C., Schnepf H.E., Whiteley H.R.
J. Biol. Chem. 258:1960-1967(1983) [PubMed: 6296116] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-333, PROTEIN SEQUENCE OF 1-9.
Strain: HD-1-Dippel.
[4]"Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation."
Grochulski P., Masson L., Borisova S., Pusztai-Carey M., Schwartz J.L., Brousseau R., Cygler M.
J. Mol. Biol. 254:447-464(1995) [PubMed: 7490762] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 33-609.
Strain: HD-1-Dippel.

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Cross-references

Sequence databases

M11250 Genomic DNA. Translation: AAA22353.1.
Y09663 mRNA. Translation: CAA70856.1.
J01554 Genomic DNA. No translation available.
PIRA22617.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1CIYX-ray2.25A29-618[»]
SMRP0A366. Positions 33-609.
ModBaseSearch...

Family and domain databases

InterProIPR005638. Endotoxin_C.
IPR001178. Endotoxin_M.
IPR015790. Endotoxin_m_sub_1.
IPR005639. Endotoxin_N.
[Graphical view]
Gene3DG3DSA:2.100.10.10. Endotoxin_M. 1 hit.
G3DSA:1.20.190.10. Endotoxin_N. 1 hit.
PfamPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameCR1AA_BACTK
AccessionPrimary (citable) accession number: P0A366
Secondary accession number(s): P02965 expand/collapse secondary AC list , P09664, P09665, P16478, Q9RED5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 15, 2005
Last modified: June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents