P0A366 (CR1AA_BACTK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pesticidal crystal protein cry1Aa

Alternative name(s):
133 kDa crystal protein
Crystaline entomocidal protoxin
Insecticidal delta-endotoxin CryIA(a)

Gene names

Name:	cry1Aa
Synonyms:	cry-1-1, cry1A(a), cryA, crybns3-1, cryIA(a), icp

Organism

Bacillus thuringiensis subsp. kurstaki

Taxonomic identifier

29339 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus thuringiensis

Protein attributes

Sequence length	1176 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.
Developmental stage	The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.
Miscellaneous	Toxic segment of the protein is located in the N-terminus.
Sequence similarities	Belongs to the delta endotoxin family.

Ontologies

Keywords
Biological process	Sporulation
Molecular function	Toxin
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: InterPro sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1176

1176

Pesticidal crystal protein cry1Aa

PRO_0000174019

Natural variations

Natural variant

77

1

P → L in strain: BNS3.

Experimental info

Sequence conflict

1009

1

V → L in AAA22353. Ref.1

Secondary structure

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1176

Helix Strand Turn

Details...

Helix

35 – 48

14

Beta strand

51 – 53

3

Helix

54 – 63

10

Helix

70 – 84

15

Helix

90 – 119

30

Helix

124 – 144

21

Helix

145 – 148

4

Helix

154 – 178

25

Helix

180 – 182

3

Helix

186 – 218

33

Helix

223 – 239

17

Helix

241 – 244

4

Helix

245 – 250

6

Turn

252 – 254

3

Beta strand

266 – 269

4

Helix

271 – 274

4

Helix

284 – 289

6

Beta strand

298 – 310

13

Beta strand

313 – 325

13

Helix

326 – 328

3

Beta strand

344 – 351

8

Beta strand

357 – 367

11

Beta strand

380 – 390

11

Beta strand

393 – 395

3

Beta strand

400 – 403

4

Beta strand

407 – 409

3

Helix

410 – 412

3

Helix

423 – 426

4

Beta strand

429 – 434

6

Beta strand

444 – 449

6

Beta strand

452 – 456

5

Beta strand

467 – 474

8

Helix

475 – 477

3

Beta strand

479 – 481

3

Beta strand

486 – 488

3

Beta strand

492 – 496

5

Beta strand

498 – 514

17

Beta strand

522 – 532

11

Beta strand

534 – 540

7

Beta strand

543 – 550

8

Helix

562 – 564

3

Beta strand

566 – 569

4

Beta strand

577 – 590

14

Beta strand

596 – 605

10

Sequences

	Sequence		Length	Mass (Da)
	P0A366 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: E2EE15AF12E5DD85	FASTA	1,176	133,120
	10 20 30 40 50 60 MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL 70 80 90 100 110 120 VDIIWGIFGP SQWDAFPVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD 130 140 150 160 170 180 PTNPALREEM RIQFNDMNSA LTTAIPLLAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ 190 200 210 220 230 240 RWGFDAATIN SRYNDLTRLI GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV 250 260 270 280 290 300 LDIVALFSNY DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGMAQRIEQN IRQPHLMDIL 310 320 330 340 350 360 NSITIYTDVH RGFNYWSGHQ ITASPVGFSG PEFAFPLFGN AGNAAPPVLV SLTGLGIFRT 370 380 390 400 410 420 LSSPLYRRII LGSGPNNQEL FVLDGTEFSF ASLTTNLPST IYRQRGTVDS LDVIPPQDNS 430 440 450 460 470 480 VPPRAGFSHR LSHVTMLSQA AGAVYTLRAP TFSWQHRSAE FNNIIPSSQI TQIPLTKSTN 490 500 510 520 530 540 LGSGTSVVKG PGFTGGDILR RTSPGQISTL RVNITAPLSQ RYRVRIRYAS TTNLQFHTSI 550 560 570 580 590 600 DGRPINQGNF SATMSSGSNL QSGSFRTVGF TTPFNFSNGS SVFTLSAHVF NSGNEVYIDR 610 620 630 640 650 660 IEFVPAEVTF EAEYDLERAQ KAVNELFTSS NQIGLKTDVT DYHIDQVSNL VECLSDEFCL 670 680 690 700 710 720 DEKQELSEKV KHAKRLSDER NLLQDPNFRG INRQLDRGWR GSTDITIQGG DDVFKENYVT 730 740 750 760 770 780 LLGTFDECYP TYLYQKIDES KLKAYTRYQL RGYIEDSQDL EIYLIRYNAK HETVNVPGTG 790 800 810 820 830 840 SLWPLSAQSP IGKCGEPNRC APHLEWNPDL DCSCRDGEKC AHHSHHFSLD IDVGCTDLNE 850 860 870 880 890 900 DLGVWVIFKI KTQDGHARLG NLEFLEEKPL VGEALARVKR AEKKWRDKRE KLEWETNIVY 910 920 930 940 950 960 KEAKESVDAL FVNSQYDQLQ ADTNIAMIHA ADKRVHSIRE AYLPELSVIP GVNAAIFEEL 970 980 990 1000 1010 1020 EGRIFTAFSL YDARNVIKNG DFNNGLSCWN VKGHVDVEEQ NNQRSVLVVP EWEAEVSQEV 1030 1040 1050 1060 1070 1080 RVCPGRGYIL RVTAYKEGYG EGCVTIHEIE NNTDELKFSN CVEEEIYPNN TVTCNDYTVN 1090 1100 1110 1120 1130 1140 QEEYGGAYTS RNRGYNEAPS VPADYASVYE EKSYTDGRRE NPCEFNRGYR DYTPLPVGYV 1150 1160 1170 TKELEYFPET DKVWIEIGET EGTFIVDSVE LLLMEE

References

[1]	"The amino acid sequence of a crystal protein from Bacillus thuringiensis deduced from the DNA base sequence." Schnepf H.E., Wong H.C., Whiteley H.R. J. Biol. Chem. 260:6264-6272(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HD-1-Dippel.
[2]	"Cloning and nucleotide sequence of a novel cry1Aa-type gene from Bacillus thuringiensis subsp.kurstaki." Tounsi S., J'Mal A., Zouari N., Jaoua S. Biotechnol. Lett. 21:771-775(1999) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BNS3.
[3]	"Transcriptional and translational start sites for the Bacillus thuringiensis crystal protein gene." Wong H.C., Schnepf H.E., Whiteley H.R. J. Biol. Chem. 258:1960-1967(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-333, PROTEIN SEQUENCE OF 1-9. Strain: HD-1-Dippel.
[4]	"Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation." Grochulski P., Masson L., Borisova S., Pusztai-Carey M., Schwartz J.L., Brousseau R., Cygler M. J. Mol. Biol. 254:447-464(1995) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 33-609. Strain: HD-1-Dippel.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M11250 Genomic DNA. Translation: AAA22353.1.
Y09663 mRNA. Translation: CAA70856.1.
J01554 Genomic DNA. No translation available.

PIR

A22617.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CIY	X-ray	2.25	A	29-618	[»]

ProteinModelPortal

P0A366.

SMR

P0A366. Positions 33-609.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-7259645.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.20.190.10. 1 hit.
2.100.10.10. 1 hit.

InterPro

IPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]

Pfam

PF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]

SUPFAM

SSF51096. Endotoxin. 1 hit.
SSF56849. Endotoxin_N. 1 hit.
SSF49785. Gal_bind_like. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0A366.

Entry information

Entry name

CR1AA_BACTK

Accession

Primary (citable) accession number: P0A366
Secondary accession number(s): P02965 , P09664, P09665, P16478, Q9RED5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	March 15, 2005
Last modified:	April 3, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program