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Protein

pH-gated potassium channel KcsA

Gene

kcsA

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
pH-gated potassium channel KcsA
Gene namesi
Name:kcsA
Synonyms:skc1
Ordered Locus Names:SCO7660
ORF Names:SC10F4.33
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2727CytoplasmicBy similarityAdd
BLAST
Transmembranei28 – 5023HelicalBy similarityAdd
BLAST
Topological domaini51 – 6111ExtracellularBy similarityAdd
BLAST
Intramembranei62 – 7211Helical; Pore-formingSequence analysisAdd
BLAST
Intramembranei73 – 808Pore-formingSequence analysis
Topological domaini81 – 877ExtracellularBy similarity
Transmembranei88 – 11124HelicalBy similarityAdd
BLAST
Topological domaini112 – 16049CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 160160pH-gated potassium channel KcsAPRO_0000054101Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi100226.SCO7660.

Structurei

Secondary structure

1
160
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi24 – 5128Combined sources
Helixi62 – 7312Combined sources
Beta strandi79 – 813Combined sources
Helixi86 – 12136Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S5HX-ray2.20C3-124[»]
2HFEX-ray2.25C22-78[»]
D80-122[»]
ProteinModelPortaliP0A333.
SMRiP0A333. Positions 22-124.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A333.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi75 – 806Selectivity filter

Domaini

The cytoplasmic C-terminus is involved in the gating mechanism.By similarity

Sequence similaritiesi

Belongs to the potassium channel family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226. LUCA.
HOGENOMiHOG000113212.
InParanoidiP0A333.
KOiK10716.
OMAiLWWSVET.
OrthoDBiPOG091H04JJ.
PhylomeDBiP0A333.

Family and domain databases

InterProiIPR013099. K_chnl_dom.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF07885. Ion_trans_2. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.

Sequencei

Sequence statusi: Complete.

P0A333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPMLSGLLA RLVKLLLGRH GSALHWRAAG AATVLLVIVL LAGSYLAVLA
60 70 80 90 100
ERGAPGAQLI TYPRALWWSV ETATTVGYGD LYPVTLWGRL VAVVVMVAGI
110 120 130 140 150
TSFGLVTAAL ATWFVGREQE RRGHFVRHSE KAAEEAYTRT TRALHERFDR
160
LERMLDDNRR
Length:160
Mass (Da):17,694
Last modified:March 15, 2005 - v1
Checksum:iDEBD9E64384BF40C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939132 Genomic DNA. Translation: CAC16993.1.
RefSeqiNP_631700.1. NC_003888.3.
WP_003971485.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAC16993; CAC16993; CAC16993.
GeneIDi1103098.
KEGGisco:SCO7660.
PATRICi23745273. VBIStrCoe124346_7779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939132 Genomic DNA. Translation: CAC16993.1.
RefSeqiNP_631700.1. NC_003888.3.
WP_003971485.1. NC_003888.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S5HX-ray2.20C3-124[»]
2HFEX-ray2.25C22-78[»]
D80-122[»]
ProteinModelPortaliP0A333.
SMRiP0A333. Positions 22-124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi100226.SCO7660.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC16993; CAC16993; CAC16993.
GeneIDi1103098.
KEGGisco:SCO7660.
PATRICi23745273. VBIStrCoe124346_7779.

Phylogenomic databases

eggNOGiCOG1226. LUCA.
HOGENOMiHOG000113212.
InParanoidiP0A333.
KOiK10716.
OMAiLWWSVET.
OrthoDBiPOG091H04JJ.
PhylomeDBiP0A333.

Miscellaneous databases

EvolutionaryTraceiP0A333.

Family and domain databases

InterProiIPR013099. K_chnl_dom.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF07885. Ion_trans_2. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
ProtoNetiSearch...

Entry informationi

Entry nameiKCSA_STRCO
AccessioniPrimary (citable) accession number: P0A333
Secondary accession number(s): Q54397
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: September 7, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The amino acids 62-79 are situated in the membrane and are important for channel structure and properties.By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.