Reviewed, UniProtKB/Swiss-Prot P0A327 (CATA_BRUAB)

Names and origin

Protein names

Recommended name:
    Catalase
    EC=1.11.1.6

Gene names

Name:	katA
Ordered Locus Names:	BruAb2_0827

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
Catalytic activity	2 H2O2 = O2 + 2 H2O.
Cofactor	Heme group.
Subunit structure	Homotetramer.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the catalase family.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Periplasm
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	catalase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 499	498	Catalase		PRO_0000084980
Sites
	Active site	55	1	By similarity
	Active site	127	1	By similarity
	Metal binding	337	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A327-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8AC86DEC18F87648	FASTA	499	56,446
	10 20 30 40 50 60 MTDRPIMTTS AGAPIPDNQN SLTAGERGPI LMQDYQLIEK LSHQNRERIP ERAVHAKGWG 70 80 90 100 110 120 AYGTLTITGD ISRYTKAKVL QPGAQTPMLA RFSTVAGELG AADAERDVRG FALKFYTQEG 130 140 150 160 170 180 NWDLVGNNTP VFFVRDPLKF PDFIHTQKRH PRTHLRSATA MWDFWSLSPE SLHQVTILMS 190 200 210 220 230 240 DRGLPTDVRH INGYGSHTYS FWNDAGERYW VKFHFKTMQG HKHWTNAEAE QVIGRTREST 250 260 270 280 290 300 QEDLFSAIEN GEFPKWKVQV QIMPELDADK TPYNPFDLTK VWPHADYPPI DIGVMELNRN 310 320 330 340 350 360 PENYFTEVEN AAFSPSNIVP GIGFSPDKML QARIFSYADA HRHRLGTHYE SIPVNQPKCP 370 380 390 400 410 420 VHHYHRDGQM NVYGGIKTGN PDAYYEPNSF NGPVEQPSAK EPPLCISGNA DRYNHRIGND 430 440 450 460 470 480 DYSQPRALFN LFDAAQKQRL FSNIAAAMKG VPGFIVERQL GHFKLIHPEY EAGVRKALKD 490 AHGYDANTIA LNEKITAAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Brucella abortus catalase is a periplasmic protein lacking a standard signal sequence." Sha Z., Stabel T.J., Mayfield J.E. J. Bacteriol. 176:7375-7377(1994) [PubMed: 7961511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11. Strain: 19.
[2]	"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U11439 Genomic DNA. Translation: AAA64655.1. AE017224 Genomic DNA. Translation: AAX76220.1.
PIR	A55227.
RefSeq	YP_223581.1.
3D structure databases
HSSP	HSSP built from PDB template 1M85 based on UniProtKB P42321.
ModBase	Search...
Genome annotation databases
GeneID	3341589.
GenomeReviews	Gene locus BruAb2_0827 in contig AE017224_GR.
KEGG	bmb:BruAb2_0827.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A327.
OMA	TQKRHPK.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB2_0827-MON.
BRENDA	1.11.1.6. 575.
Family and domain databases
InterPro	IPR002226. Catalase. IPR010582. Catalase-rel_immune_responsive. IPR011614. Catalase_N. IPR018028. Catalase_rel_subgroup. [Graphical view]
Gene3D	G3DSA:2.40.180.10. Catalase_N. 1 hit.
PANTHER	PTHR11465. Catalase. 1 hit.
Pfam	PF00199. Catalase. 1 hit. PF06628. Catalase-rel. 1 hit. [Graphical view]
PRINTS	PR00067. CATALASE.
ProDom	PD000510. Catalase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00437. CATALASE_1. 1 hit. PS00438. CATALASE_2. False negative. PS51402. CATALASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATA_BRUAB

Accession

Primary (citable) accession number: P0A327
Secondary accession number(s): Q577G4, Q59170

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)