P0A2Y5 (AROA_ACHSL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:

aroA

Organism

Achromobacter sp. (strain LBAA)

Taxonomic identifier

129026 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Achromobacter

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Miscellaneous	Resistant to the antibiotic glyphosate. HAMAP MF_00210
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Herbicide resistance
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW response to herbicide Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088213

Sequences

	Sequence		Length	Mass (Da)
	P0A2Y5 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 447F213EECCAEFC1	FASTA	449	47,297
	10 20 30 40 50 60 MSHSASPKPA TARRSEALTG EIRIPGDKSI SHRSFMFGGL ASGETRITGL LEGEDVINTG 70 80 90 100 110 120 RAMQAMGAKI RKEGDVWIIN GVGNGCLLQP EAALDFGNAG TGARLTMGLV GTYDMKTSFI 130 140 150 160 170 180 GDASLSKRPM GRVLNPLREM GVQVEAADGD RMPLTLIGPK TANPITYRVP MASAQVKSAV 190 200 210 220 230 240 LLAGLNTPGV TTVIEPVMTR DHTEKMLQGF GADLTVETDK DGVRHIRITG QGKLVGQTID 250 260 270 280 290 300 VPGDPSSTAF PLVAALLVEG SDVTIRNVLM NPTRTGLILT LQEMGADIEV LNARLAGGED 310 320 330 340 350 360 VADLRVRASK LKGVVVPPER APSMIDEYPV LAIAASFAEG ETVMDGLDEL RVKESDRLAA 370 380 390 400 410 420 VARGLEANGV DCTEGEMSLT VRGRPDGKGL GGGTVATHLD HRIAMSFLVM GLAAEKPVTV 430 440 DDSNMIATSF PEFMDMMPGL GAKIELSIL

References

[1]	"Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases." Barry G.F., Kishore G.M., Padgette S.R., Stallings W.C. Patent number US5633435, 27-MAY-1997 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16.

Cross-references

3D structure databases
ProteinModelPortal	P0A2Y5.
SMR	P0A2Y5. Positions 6-447.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_ACHSL

Accession

Primary (citable) accession number: P0A2Y5
Secondary accession number(s): P56952

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 31, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program