Reviewed, UniProtKB/Swiss-Prot P0A2X9 (ARGI_BRUME)

Names and origin

Protein names

Recommended name:
    Arginase
    EC=3.5.3.1

Gene names

Name:	arcB
Ordered Locus Names:	BMEII0396

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-arginine + H2O = L-ornithine + urea.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Sequence similarities	Belongs to the arginase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arginase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Arginase		PRO_0000173717
Regions
	Region	125 – 129	5	Substrate binding By similarity
	Region	136 – 138	3	Substrate binding By similarity
Sites
	Metal binding	96	1	Manganese 1 By similarity
	Metal binding	123	1	Manganese 1 By similarity
	Metal binding	123	1	Manganese 2 By similarity
	Metal binding	125	1	Manganese 2 By similarity
	Metal binding	127	1	Manganese 1 By similarity
	Metal binding	226	1	Manganese 1 By similarity
	Metal binding	226	1	Manganese 2 By similarity
	Metal binding	228	1	Manganese 2 By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	271	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A2X9-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: A953867BC04570B3	FASTA	306	33,182
	10 20 30 40 50 60 MHCKILGLPV QEGTGRKGCN MGPDSYRAAG IADAIRELGH ECTDLGNLAP AAQRPLQHPN 70 80 90 100 110 120 HAIKALPYAV AWIEAISEAA YRESAEGFPI FLGGDHLLAA GTVPGIARRA AEKGRKQFVL 130 140 150 160 170 180 WLDAHTDFHT LETTTSGNLH GTPVAYYTGQ KGFEGYFPKL AAPIDPHNVC MLGIRSVDPA 190 200 210 220 230 240 EREAVKKTEV IVYDMRLIDE HGVAALLRRF LERVKAEDGL LHVSLDVDFL DPSIAPAVGT 250 260 270 280 290 300 TVPGGATFRE AHLIMEMLHD SGLVTSLDLV ELNPFLDERG RTAAVMVDLM ASLLGRSVMD RPTISY

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008918 Genomic DNA. Translation: AAL53638.1. Different initiation.
PIR	AC3559.
RefSeq	NP_541374.1.
3D structure databases
HSSP	HSSP built from PDB template 2CEV based on UniProtKB P53608.
ModBase	Search...
Genome annotation databases
GeneID	1198168.
GenomeReviews	Gene locus BMEII0396 in contig AE008918_GR.
KEGG	bme:BMEII0396.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A2X9.
OMA	TYREAQL.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEII0396-MON.
BRENDA	3.5.3.1. 277959.
Family and domain databases
InterPro	IPR005924. Arginase. IPR014033. Arginase_sub. IPR006035. Ureohydrolase. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
PANTHER	PTHR11358:SF2. Arginase_sub. 1 hit. PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PRINTS	PR00116. ARGINASE.
TIGRFAMs	TIGR01229. rocF_arginase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGI_BRUME

Accession

Primary (citable) accession number: P0A2X9
Secondary accession number(s): Q59174

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)