P0A2X9 (ARGI_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginase
EC=3.5.3.1

Gene names

Name:	arcB
Ordered Locus Names:	BMEII0396

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-arginine + H2O = L-ornithine + urea.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Sequence similarities	Belongs to the arginase family.
Sequence caution	The sequence AAL53638.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arginase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Arginase		PRO_0000173717
Regions
	Region	125 – 129	5	Substrate binding By similarity
	Region	136 – 138	3	Substrate binding By similarity
Sites
	Metal binding	96	1	Manganese 1 By similarity
	Metal binding	123	1	Manganese 1 By similarity
	Metal binding	123	1	Manganese 2 By similarity
	Metal binding	125	1	Manganese 2 By similarity
	Metal binding	127	1	Manganese 1 By similarity
	Metal binding	226	1	Manganese 1 By similarity
	Metal binding	226	1	Manganese 2 By similarity
	Metal binding	228	1	Manganese 2 By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	271	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A2X9 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: A953867BC04570B3	FASTA	306	33,182
	10 20 30 40 50 60 MHCKILGLPV QEGTGRKGCN MGPDSYRAAG IADAIRELGH ECTDLGNLAP AAQRPLQHPN 70 80 90 100 110 120 HAIKALPYAV AWIEAISEAA YRESAEGFPI FLGGDHLLAA GTVPGIARRA AEKGRKQFVL 130 140 150 160 170 180 WLDAHTDFHT LETTTSGNLH GTPVAYYTGQ KGFEGYFPKL AAPIDPHNVC MLGIRSVDPA 190 200 210 220 230 240 EREAVKKTEV IVYDMRLIDE HGVAALLRRF LERVKAEDGL LHVSLDVDFL DPSIAPAVGT 250 260 270 280 290 300 TVPGGATFRE AHLIMEMLHD SGLVTSLDLV ELNPFLDERG RTAAVMVDLM ASLLGRSVMD RPTISY

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008918 Genomic DNA. Translation: AAL53638.1. Different initiation.
PIR	AC3559.
RefSeq	NP_541374.1. NC_003318.1.
3D structure databases
ProteinModelPortal	P0A2X9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1198168.
GenomeReviews	Gene locus BMEII0396 in contig AE008918_GR.
KEGG	bme:BMEII0396.
PATRIC	17855466. VBIBruMel92729_2701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391953.
OMA	IGAPTDI.
PhylomeDB	P0A2X9.
ProtClustDB	CLSK861991.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEII0396-MONOMER.
Family and domain databases
InterPro	IPR014033. Arginase_subgr. IPR006035. Ureohydrolase. IPR023696. Ureohydrolase_domain. IPR020855. Ureohydrolase_Mn_BS. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
KO	K01476.
PANTHER	PTHR11358:SF2. Arginase_sub. 1 hit. PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PIRSF	PIRSF036979. Arginase. 1 hit.
PRINTS	PR00116. ARGINASE.
TIGRFAMs	TIGR01229. RocF_arginase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGI_BRUME

Accession

Primary (citable) accession number: P0A2X9
Secondary accession number(s): Q59174

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program