P0A2X4 (EXOA_STRR6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exodeoxyribonuclease
EC=3.1.11.2

Gene names

Name:	exoA
Ordered Locus Names:	spr1660

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6)

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, ExoA was shown to make single-strand breaks at apurinic sites in DNA.
Catalytic activity	Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the DNA repair enzymes AP/ExoA family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: InterPro exodeoxyribonuclease III activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Exodeoxyribonuclease		PRO_0000200027
Sites
	Active site	266	1	Proton acceptor By similarity
	Metal binding	44	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A2X4 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 112E5A9FF828A1B9	FASTA	275	31,064
	10 20 30 40 50 60 MKLISWNIDS LNAALTSDSA RAKLSQEVLQ TLVAENADII AIQETKLSAK GPTKKHVEIL 70 80 90 100 110 120 EELFPGYENT WRSSQEPARK GYAGTMFLYK KELTPTISFP EIGAPSTMDL EGRIITLEFD 130 140 150 160 170 180 AFFVTQVYTP NAGDGLKRLE ERQVWDAKYA EYLAELDKEK PVLATGDYNV AHNEIDLANP 190 200 210 220 230 240 ASNRRSPGFT DEERAGFTNL LATGFTDTFR HVHGDVPERY TWWAQRSKTS KINNTGWRID 250 260 270 YWLTSNRIAD KVTKSDMIDS GARQDHTPIV LEIDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The exoA gene of Streptococcus pneumoniae and its product, a DNA exonuclease with apurinic endonuclease activity." Puyet A., Greenberg B., Lacks S.A. J. Bacteriol. 171:2278-2286(1989) [PubMed: 2468646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed: 11544234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-255 / R6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04234 Genomic DNA. Translation: AAA26879.1. AE007317 Genomic DNA. Translation: AAL00463.1.
PIR	A32301. B98079.
RefSeq	NP_359252.1. NC_003098.1.
3D structure databases
ProteinModelPortal	P0A2X4.
ModBase	Search...
Protein-protein interaction databases
STRING	P0A2X4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000015803; EBSTRP00000015199; EBSTRG00000015803.
GeneID	933227.
GenomeReviews	Gene locus spr1660 in contig AE007317_GR.
KEGG	spr:spr1660.
PATRIC	19703354. VBIStrPne107296_1792.
Phylogenomic databases
eggNOG	COG0708.
GeneTree	EBGT00050000027380.
HOGENOM	HBG704164.
OMA	MKARERN.
PhylomeDB	P0A2X4.
ProtClustDB	CLSK2752120.
Enzyme and pathway databases
BioCyc	SPNE171101:SPR1660-MONOMER.
Family and domain databases
InterPro	IPR000097. AP_endonuclease_F1. IPR020847. AP_endonuclease_F1_BS. IPR020848. AP_endonuclease_F1_CS. IPR005135. Endo/exonuclease/phosphatase. IPR004808. ExoDNase_III. [Graphical view]
KO	K01142.
PANTHER	PTHR22748. ExoIII_xth. 1 hit.
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
TIGRFAMs	TIGR00195. ExoDNase_III. 1 hit. TIGR00633. Xth. 1 hit.
PROSITE	PS00726. AP_NUCLEASE_F1_1. 1 hit. PS00727. AP_NUCLEASE_F1_2. 1 hit. PS00728. AP_NUCLEASE_F1_3. 1 hit. PS51435. AP_NUCLEASE_F1_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EXOA_STRR6

Accession

Primary (citable) accession number: P0A2X4
Secondary accession number(s): P21998

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program