Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0A2X3

- EXOA_STRPN

UniProt

P0A2X3 - EXOA_STRPN

Protein

Exodeoxyribonuclease

Gene

exoA

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, ExoA was shown to make single-strand breaks at apurinic sites in DNA.By similarity

    Catalytic activityi

    Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

    Cofactori

    Magnesium. Can also utilize manganese. Probably binds two magnesium or manganese ions per subunit By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Magnesium 1By similarity
    Metal bindingi44 – 441Magnesium 1By similarity
    Active sitei128 – 1281By similarity
    Active sitei167 – 1671Proton donor/acceptorBy similarity
    Metal bindingi167 – 1671Magnesium 2By similarity
    Metal bindingi169 – 1691Magnesium 2By similarity
    Sitei169 – 1691Transition state stabilizerBy similarity
    Sitei240 – 2401Important for catalytic activityBy similarity
    Metal bindingi265 – 2651Magnesium 1By similarity
    Sitei266 – 2661Interaction with DNA substrateBy similarity

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. endonuclease activity Source: InterPro
    3. exodeoxyribonuclease III activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA repair Source: InterPro

    Keywords - Molecular functioni

    Exonuclease, Hydrolase, Nuclease

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciSPNE170187:GHGN-1853-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exodeoxyribonuclease (EC:3.1.11.2)
    Gene namesi
    Name:exoA
    Ordered Locus Names:SP_1845
    OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
    Taxonomic identifieri170187 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000585: Chromosome

    Subcellular locationi

    Cytoplasm PROSITE-ProRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 275275ExodeoxyribonucleasePRO_0000200026Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi170187.SP_1845.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A2X3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DNA repair enzymes AP/ExoA family.Curated

    Phylogenomic databases

    eggNOGiCOG0708.
    HOGENOMiHOG000034586.
    KOiK01142.
    OMAiGWRIDYY.
    OrthoDBiEOG6TBHJS.

    Family and domain databases

    Gene3Di3.60.10.10. 1 hit.
    InterProiIPR004808. AP_endonuc_1.
    IPR020847. AP_endonuclease_F1_BS.
    IPR020848. AP_endonuclease_F1_CS.
    IPR005135. Endo/exonuclease/phosphatase.
    [Graphical view]
    PANTHERiPTHR22748. PTHR22748. 1 hit.
    PfamiPF03372. Exo_endo_phos. 1 hit.
    [Graphical view]
    SUPFAMiSSF56219. SSF56219. 1 hit.
    TIGRFAMsiTIGR00633. xth. 1 hit.
    PROSITEiPS00726. AP_NUCLEASE_F1_1. 1 hit.
    PS00727. AP_NUCLEASE_F1_2. 1 hit.
    PS00728. AP_NUCLEASE_F1_3. 1 hit.
    PS51435. AP_NUCLEASE_F1_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A2X3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLISWNIDS LNAALTSDSA RAKLSQEVLQ TLVAENADII AIQETKLSAK    50
    GPTKKHVEIL EELFPGYENT WRSSQEPARK GYAGTMFLYK KELTPTISFP 100
    EIGAPSTMDL EGRIITLEFD AFFVTQVYTP NAGDGLKRLE ERQVWDAKYA 150
    EYLAELDKEK PVLATGDYNV AHNEIDLANP ASNRRSPGFT DEERAGFTNL 200
    LATGFTDTFR HVHGDVPERY TWWAQRSKTS KINNTGWRID YWLTSNRIAD 250
    KVTKSDMIDS GARQDHTPIV LEIDL 275
    Length:275
    Mass (Da):31,064
    Last modified:March 15, 2005 - v1
    Checksum:i112E5A9FF828A1B9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005672 Genomic DNA. Translation: AAK75918.1.
    PIRiE95215.
    RefSeqiNP_346278.1. NC_003028.3.

    Genome annotation databases

    EnsemblBacteriaiAAK75918; AAK75918; SP_1845.
    GeneIDi930987.
    KEGGispn:SP_1845.
    PATRICi19708143. VBIStrPne105772_1914.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005672 Genomic DNA. Translation: AAK75918.1 .
    PIRi E95215.
    RefSeqi NP_346278.1. NC_003028.3.

    3D structure databases

    ProteinModelPortali P0A2X3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 170187.SP_1845.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK75918 ; AAK75918 ; SP_1845 .
    GeneIDi 930987.
    KEGGi spn:SP_1845.
    PATRICi 19708143. VBIStrPne105772_1914.

    Phylogenomic databases

    eggNOGi COG0708.
    HOGENOMi HOG000034586.
    KOi K01142.
    OMAi GWRIDYY.
    OrthoDBi EOG6TBHJS.

    Enzyme and pathway databases

    BioCyci SPNE170187:GHGN-1853-MONOMER.

    Family and domain databases

    Gene3Di 3.60.10.10. 1 hit.
    InterProi IPR004808. AP_endonuc_1.
    IPR020847. AP_endonuclease_F1_BS.
    IPR020848. AP_endonuclease_F1_CS.
    IPR005135. Endo/exonuclease/phosphatase.
    [Graphical view ]
    PANTHERi PTHR22748. PTHR22748. 1 hit.
    Pfami PF03372. Exo_endo_phos. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56219. SSF56219. 1 hit.
    TIGRFAMsi TIGR00633. xth. 1 hit.
    PROSITEi PS00726. AP_NUCLEASE_F1_1. 1 hit.
    PS00727. AP_NUCLEASE_F1_2. 1 hit.
    PS00728. AP_NUCLEASE_F1_3. 1 hit.
    PS51435. AP_NUCLEASE_F1_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-334 / TIGR4.

    Entry informationi

    Entry nameiEXOA_STRPN
    AccessioniPrimary (citable) accession number: P0A2X3
    Secondary accession number(s): P21998
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3