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Protein

Exodeoxyribonuclease

Gene

exoA

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, ExoA was shown to make single-strand breaks at apurinic sites in DNA.By similarity

Catalytic activityi

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Probably binds two magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9Magnesium 1By similarity1
Metal bindingi44Magnesium 1By similarity1
Active sitei128By similarity1
Active sitei167Proton donor/acceptorBy similarity1
Metal bindingi167Magnesium 2By similarity1
Metal bindingi169Magnesium 2By similarity1
Sitei169Transition state stabilizerBy similarity1
Sitei240Important for catalytic activityBy similarity1
Metal bindingi265Magnesium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Exodeoxyribonuclease (EC:3.1.11.2)
Gene namesi
Name:exoA
Ordered Locus Names:SP_1845
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002000261 – 275ExodeoxyribonucleaseAdd BLAST275

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei266Interaction with DNA substrateBy similarity1

Protein-protein interaction databases

STRINGi170187.SpneT_02000620.

Structurei

3D structure databases

ProteinModelPortaliP0A2X3.
SMRiP0A2X3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA repair enzymes AP/ExoA family.Curated

Phylogenomic databases

eggNOGiENOG4105CGK. Bacteria.
COG0708. LUCA.
HOGENOMiHOG000034586.
KOiK01142.
OMAiWRIDYYL.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR004808. AP_endonuc_1.
IPR020847. AP_endonuclease_F1_BS.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR22748. PTHR22748. 1 hit.
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
TIGRFAMsiTIGR00633. xth. 1 hit.
PROSITEiPS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A2X3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLISWNIDS LNAALTSDSA RAKLSQEVLQ TLVAENADII AIQETKLSAK
60 70 80 90 100
GPTKKHVEIL EELFPGYENT WRSSQEPARK GYAGTMFLYK KELTPTISFP
110 120 130 140 150
EIGAPSTMDL EGRIITLEFD AFFVTQVYTP NAGDGLKRLE ERQVWDAKYA
160 170 180 190 200
EYLAELDKEK PVLATGDYNV AHNEIDLANP ASNRRSPGFT DEERAGFTNL
210 220 230 240 250
LATGFTDTFR HVHGDVPERY TWWAQRSKTS KINNTGWRID YWLTSNRIAD
260 270
KVTKSDMIDS GARQDHTPIV LEIDL
Length:275
Mass (Da):31,064
Last modified:March 15, 2005 - v1
Checksum:i112E5A9FF828A1B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK75918.1.
PIRiE95215.
RefSeqiWP_000767464.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK75918; AAK75918; SP_1845.
KEGGispn:SP_1845.
PATRICi19708143. VBIStrPne105772_1914.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK75918.1.
PIRiE95215.
RefSeqiWP_000767464.1. NZ_AKVY01000001.1.

3D structure databases

ProteinModelPortaliP0A2X3.
SMRiP0A2X3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi170187.SpneT_02000620.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK75918; AAK75918; SP_1845.
KEGGispn:SP_1845.
PATRICi19708143. VBIStrPne105772_1914.

Phylogenomic databases

eggNOGiENOG4105CGK. Bacteria.
COG0708. LUCA.
HOGENOMiHOG000034586.
KOiK01142.
OMAiWRIDYYL.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR004808. AP_endonuc_1.
IPR020847. AP_endonuclease_F1_BS.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR22748. PTHR22748. 1 hit.
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
TIGRFAMsiTIGR00633. xth. 1 hit.
PROSITEiPS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEXOA_STRPN
AccessioniPrimary (citable) accession number: P0A2X3
Secondary accession number(s): P21998
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.