Reviewed, UniProtKB/Swiss-Prot P0A2X3 (EXOA_STRPN)

Names and origin

Protein names

Recommended name:
    Exodeoxyribonuclease
    EC=3.1.11.2

Gene names

Name:	exoA
Ordered Locus Names:	SP_1845

Organism

Streptococcus pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, exoA was shown to make single-strand breaks at apurinic sites in DNA By similarity.
Catalytic activity	Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DNA repair enzymes AP/exoA family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: InterPro exodeoxyribonuclease III activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Exodeoxyribonuclease		PRO_0000200026
Sites
	Active site	266	1	Proton acceptor By similarity
	Metal binding	44	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A2X3-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 112E5A9FF828A1B9	FASTA	275	31,064
	10 20 30 40 50 60 MKLISWNIDS LNAALTSDSA RAKLSQEVLQ TLVAENADII AIQETKLSAK GPTKKHVEIL 70 80 90 100 110 120 EELFPGYENT WRSSQEPARK GYAGTMFLYK KELTPTISFP EIGAPSTMDL EGRIITLEFD 130 140 150 160 170 180 AFFVTQVYTP NAGDGLKRLE ERQVWDAKYA EYLAELDKEK PVLATGDYNV AHNEIDLANP 190 200 210 220 230 240 ASNRRSPGFT DEERAGFTNL LATGFTDTFR HVHGDVPERY TWWAQRSKTS KINNTGWRID 250 260 270 YWLTSNRIAD KVTKSDMIDS GARQDHTPIV LEIDL

References

[1]

"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed: 11463916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005672 Genomic DNA. Translation: AAK75918.1.
PIR	E95215.
RefSeq	NP_346278.1.
3D structure databases
HSSP	HSSP built from PDB template 1E9N based on UniProtKB P27695.
ModBase	Search...
Genome annotation databases
GeneID	930987.
GenomeReviews	Gene locus SP_1845 in contig AE005672_GR.
KEGG	spn:SP_1845.
TIGR	SP_1845.
Phylogenomic databases
HOGENOM	P0A2X3.
OMA	TGPTKKH.
Enzyme and pathway databases
BRENDA	3.1.11.2. 600.
Family and domain databases
InterPro	IPR000097. AP_endonuclease_F1. IPR005135. Endo/exonuclease/phosphatase. IPR004808. exoDNase_III. [Graphical view]
PANTHER	PTHR22748. ExoIII_xth. 1 hit.
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
TIGRFAMs	TIGR00195. exoDNase_III. 1 hit. TIGR00633. xth. 1 hit.
PROSITE	PS00726. AP_NUCLEASE_F1_1. 1 hit. PS00727. AP_NUCLEASE_F1_2. 1 hit. PS00728. AP_NUCLEASE_F1_3. 1 hit. PS51435. AP_NUCLEASE_F1_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EXOA_STRPN

Accession

Primary (citable) accession number: P0A2X3
Secondary accession number(s): P21998

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)