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Protein

Exodeoxyribonuclease

Gene

exoA

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, ExoA was shown to make single-strand breaks at apurinic sites in DNA.By similarity

Catalytic activityi

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Probably binds two magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1By similarity
Metal bindingi44 – 441Magnesium 1By similarity
Active sitei128 – 1281By similarity
Active sitei167 – 1671Proton donor/acceptorBy similarity
Metal bindingi167 – 1671Magnesium 2By similarity
Metal bindingi169 – 1691Magnesium 2By similarity
Sitei169 – 1691Transition state stabilizerBy similarity
Sitei240 – 2401Important for catalytic activityBy similarity
Metal bindingi265 – 2651Magnesium 1By similarity
Sitei266 – 2661Interaction with DNA substrateBy similarity

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. endonuclease activity Source: InterPro
  3. exodeoxyribonuclease III activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-1853-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exodeoxyribonuclease (EC:3.1.11.2)
Gene namesi
Name:exoA
Ordered Locus Names:SP_1845
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000585 Componenti: Chromosome

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275ExodeoxyribonucleasePRO_0000200026Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi170187.SP_1845.

Structurei

3D structure databases

ProteinModelPortaliP0A2X3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA repair enzymes AP/ExoA family.Curated

Phylogenomic databases

eggNOGiCOG0708.
HOGENOMiHOG000034586.
KOiK01142.
OMAiWLASNRI.
OrthoDBiEOG6TBHJS.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR004808. AP_endonuc_1.
IPR020847. AP_endonuclease_F1_BS.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR22748. PTHR22748. 1 hit.
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
TIGRFAMsiTIGR00633. xth. 1 hit.
PROSITEiPS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A2X3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLISWNIDS LNAALTSDSA RAKLSQEVLQ TLVAENADII AIQETKLSAK
60 70 80 90 100
GPTKKHVEIL EELFPGYENT WRSSQEPARK GYAGTMFLYK KELTPTISFP
110 120 130 140 150
EIGAPSTMDL EGRIITLEFD AFFVTQVYTP NAGDGLKRLE ERQVWDAKYA
160 170 180 190 200
EYLAELDKEK PVLATGDYNV AHNEIDLANP ASNRRSPGFT DEERAGFTNL
210 220 230 240 250
LATGFTDTFR HVHGDVPERY TWWAQRSKTS KINNTGWRID YWLTSNRIAD
260 270
KVTKSDMIDS GARQDHTPIV LEIDL
Length:275
Mass (Da):31,064
Last modified:March 14, 2005 - v1
Checksum:i112E5A9FF828A1B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK75918.1.
PIRiE95215.
RefSeqiNP_346278.1. NC_003028.3.

Genome annotation databases

EnsemblBacteriaiAAK75918; AAK75918; SP_1845.
KEGGispn:SP_1845.
PATRICi19708143. VBIStrPne105772_1914.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK75918.1.
PIRiE95215.
RefSeqiNP_346278.1. NC_003028.3.

3D structure databases

ProteinModelPortaliP0A2X3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi170187.SP_1845.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK75918; AAK75918; SP_1845.
KEGGispn:SP_1845.
PATRICi19708143. VBIStrPne105772_1914.

Phylogenomic databases

eggNOGiCOG0708.
HOGENOMiHOG000034586.
KOiK01142.
OMAiWLASNRI.
OrthoDBiEOG6TBHJS.

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-1853-MONOMER.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR004808. AP_endonuc_1.
IPR020847. AP_endonuclease_F1_BS.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERiPTHR22748. PTHR22748. 1 hit.
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
TIGRFAMsiTIGR00633. xth. 1 hit.
PROSITEiPS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-334 / TIGR4.

Entry informationi

Entry nameiEXOA_STRPN
AccessioniPrimary (citable) accession number: P0A2X3
Secondary accession number(s): P21998
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 14, 2005
Last sequence update: March 14, 2005
Last modified: March 31, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.