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P0A2X3 (EXOA_STRPN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exodeoxyribonuclease

EC=3.1.11.2
Gene names
Name:exoA
Ordered Locus Names:SP_1845
OrganismStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [Complete proteome] [HAMAP]
Taxonomic identifier170187 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, ExoA was shown to make single-strand breaks at apurinic sites in DNA By similarity.

Catalytic activity

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

Cofactor

Magnesium. Can also utilize manganese. Probably binds two magnesium or manganese ions per subunit By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the DNA repair enzymes AP/ExoA family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionExonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

endonuclease activity

Inferred from electronic annotation. Source: InterPro

exodeoxyribonuclease III activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 275275Exodeoxyribonuclease
PRO_0000200026

Sites

Active site1281 By similarity
Active site1671Proton donor/acceptor By similarity
Metal binding91Magnesium 1 By similarity
Metal binding441Magnesium 1 By similarity
Metal binding1671Magnesium 2 By similarity
Metal binding1691Magnesium 2 By similarity
Metal binding2651Magnesium 1 By similarity
Site1691Transition state stabilizer By similarity
Site2401Important for catalytic activity By similarity
Site2661Interaction with DNA substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A2X3 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 112E5A9FF828A1B9

FASTA27531,064
        10         20         30         40         50         60 
MKLISWNIDS LNAALTSDSA RAKLSQEVLQ TLVAENADII AIQETKLSAK GPTKKHVEIL 

        70         80         90        100        110        120 
EELFPGYENT WRSSQEPARK GYAGTMFLYK KELTPTISFP EIGAPSTMDL EGRIITLEFD 

       130        140        150        160        170        180 
AFFVTQVYTP NAGDGLKRLE ERQVWDAKYA EYLAELDKEK PVLATGDYNV AHNEIDLANP 

       190        200        210        220        230        240 
ASNRRSPGFT DEERAGFTNL LATGFTDTFR HVHGDVPERY TWWAQRSKTS KINNTGWRID 

       250        260        270 
YWLTSNRIAD KVTKSDMIDS GARQDHTPIV LEIDL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005672 Genomic DNA. Translation: AAK75918.1.
PIRE95215.
RefSeqNP_346278.1. NC_003028.3.

3D structure databases

ProteinModelPortalP0A2X3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING170187.SP_1845.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK75918; AAK75918; SP_1845.
GeneID930987.
KEGGspn:SP_1845.
PATRIC19708143. VBIStrPne105772_1914.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0708.
HOGENOMHOG000034586.
KOK01142.
OMAGWRIDYY.
OrthoDBEOG6TBHJS.

Enzyme and pathway databases

BioCycSPNE170187:GHGN-1853-MONOMER.

Family and domain databases

Gene3D3.60.10.10. 1 hit.
InterProIPR004808. AP_endonuc_1.
IPR020847. AP_endonuclease_F1_BS.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERPTHR22748. PTHR22748. 1 hit.
PfamPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMSSF56219. SSF56219. 1 hit.
TIGRFAMsTIGR00633. xth. 1 hit.
PROSITEPS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEXOA_STRPN
AccessionPrimary (citable) accession number: P0A2X3
Secondary accession number(s): P21998
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families