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P0A2W8

- ALR_STRPN

UniProt

P0A2W8 - ALR_STRPN

Protein

Alanine racemase

Gene

alr

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

    Catalytic activityi

    L-alanine = D-alanine.UniRule annotation

    Cofactori

    Pyridoxal phosphate.1 PublicationUniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei40 – 401Proton acceptor; specific for D-alanineUniRule annotation
    Binding sitei136 – 1361SubstrateUniRule annotation
    Active sitei263 – 2631Proton acceptor; specific for L-alanineUniRule annotation
    Binding sitei310 – 3101Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. alanine racemase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. D-alanine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciSPNE170187:GHGN-1705-MONOMER.
    BRENDAi5.1.1.1. 5946.
    UniPathwayiUPA00042; UER00497.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
    Gene namesi
    Name:alr
    Synonyms:alaR
    Ordered Locus Names:SP_1698
    OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
    Taxonomic identifieri170187 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000585: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Alanine racemasePRO_0000114581Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei40 – 401N6-(pyridoxal phosphate)lysine
    Modified residuei129 – 1291N6-carboxylysine1 Publication

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi170187.SP_1698.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 145
    Helixi15 – 2713
    Beta strandi34 – 385
    Helixi40 – 445
    Helixi48 – 558
    Helixi56 – 583
    Beta strandi60 – 667
    Helixi67 – 759
    Beta strandi82 – 876
    Helixi90 – 923
    Helixi93 – 986
    Beta strandi102 – 1054
    Helixi108 – 1169
    Beta strandi125 – 1306
    Beta strandi136 – 1394
    Helixi142 – 15413
    Beta strandi158 – 1647
    Helixi175 – 18915
    Beta strandi196 – 2016
    Helixi203 – 2086
    Helixi210 – 2123
    Beta strandi215 – 2195
    Helixi221 – 2244
    Turni228 – 2314
    Beta strandi243 – 2486
    Beta strandi250 – 2556
    Beta strandi260 – 2623
    Helixi263 – 2653
    Beta strandi273 – 2797
    Helixi282 – 2843
    Helixi288 – 2903
    Beta strandi294 – 2974
    Beta strandi300 – 3045
    Beta strandi313 – 3197
    Beta strandi326 – 3338
    Beta strandi336 – 3383
    Helixi340 – 3478
    Helixi351 – 3566
    Beta strandi364 – 3674

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3S46X-ray2.00A/B1-367[»]
    ProteinModelPortaliP0A2W8.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A2W8.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alanine racemase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0787.
    HOGENOMiHOG000031444.
    KOiK01775.
    OMAiIYSHLAL.
    OrthoDBiEOG6PP9NJ.

    Family and domain databases

    Gene3Di2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPiMF_01201. Ala_racemase.
    InterProiIPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR020622. Ala_racemase_pyridoxalP-BS.
    IPR029066. PLP-binding_barrel.
    [Graphical view]
    PfamiPF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view]
    PRINTSiPR00992. ALARACEMASE.
    SMARTiSM01005. Ala_racemase_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsiTIGR00492. alr. 1 hit.
    PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A2W8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKASPHRPTK ALIHLGAIRQ NIQQMGAHIP QGTLKLAVVK ANAYGHGAVA    50
    VAKAIQDDVD GFCVSNIDEA IELRQAGLSK PILILGVSEI EAVALAKEYD 100
    FTLTVAGLEW IQALLDKEVD LTGLTVHLKI DSGMGRIGFR EASEVEQAQD 150
    LLQQHGVCVE GIFTHFATAD EESDDYFNAQ LERFKTILAS MKEVPELVHA 200
    SNSATTLWHV ETIFNAVRMG DAMYGLNPSG AVLDLPYDLI PALTLESALV 250
    HVKTVPAGAC MGYGATYQAD SEQVIATVPI GYADGWTRDM QNFSVLVDGQ 300
    ACPIVGRVSM DQITIRLPKL YPLGTKVTLI GSNGDKEITA TQVATYRVTI 350
    NYEVVCLLSD RIPREYY 367
    Length:367
    Mass (Da):39,858
    Last modified:March 15, 2005 - v1
    Checksum:iA79E2F1B439B18B7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti354 – 3541V → E in CAA90279. (PubMed:8830261)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF171873 Genomic DNA. Translation: AAD51027.1.
    AE005672 Genomic DNA. Translation: AAK75776.1.
    Z49988 Genomic DNA. Translation: CAA90279.1.
    PIRiG95197.
    S71015.
    RefSeqiNP_346136.1. NC_003028.3.
    WP_000648075.1. NZ_AKVY01000001.1.

    Genome annotation databases

    EnsemblBacteriaiAAK75776; AAK75776; SP_1698.
    GeneIDi931137.
    KEGGispn:SP_1698.
    PATRICi19707839. VBIStrPne105772_1762.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF171873 Genomic DNA. Translation: AAD51027.1 .
    AE005672 Genomic DNA. Translation: AAK75776.1 .
    Z49988 Genomic DNA. Translation: CAA90279.1 .
    PIRi G95197.
    S71015.
    RefSeqi NP_346136.1. NC_003028.3.
    WP_000648075.1. NZ_AKVY01000001.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3S46 X-ray 2.00 A/B 1-367 [» ]
    ProteinModelPortali P0A2W8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 170187.SP_1698.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK75776 ; AAK75776 ; SP_1698 .
    GeneIDi 931137.
    KEGGi spn:SP_1698.
    PATRICi 19707839. VBIStrPne105772_1762.

    Phylogenomic databases

    eggNOGi COG0787.
    HOGENOMi HOG000031444.
    KOi K01775.
    OMAi IYSHLAL.
    OrthoDBi EOG6PP9NJ.

    Enzyme and pathway databases

    UniPathwayi UPA00042 ; UER00497 .
    BioCyci SPNE170187:GHGN-1705-MONOMER.
    BRENDAi 5.1.1.1. 5946.

    Miscellaneous databases

    EvolutionaryTracei P0A2W8.

    Family and domain databases

    Gene3Di 2.40.37.10. 1 hit.
    3.20.20.10. 1 hit.
    HAMAPi MF_01201. Ala_racemase.
    InterProi IPR000821. Ala_racemase.
    IPR009006. Ala_racemase/Decarboxylase_C.
    IPR011079. Ala_racemase_C.
    IPR001608. Ala_racemase_N.
    IPR020622. Ala_racemase_pyridoxalP-BS.
    IPR029066. PLP-binding_barrel.
    [Graphical view ]
    Pfami PF00842. Ala_racemase_C. 1 hit.
    PF01168. Ala_racemase_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00992. ALARACEMASE.
    SMARTi SM01005. Ala_racemase_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50621. SSF50621. 1 hit.
    SSF51419. SSF51419. 1 hit.
    TIGRFAMsi TIGR00492. alr. 1 hit.
    PROSITEi PS00395. ALANINE_RACEMASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Guynn L.J., Strych U., Benedik M.J.
      Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-334 / TIGR4.
    3. "The mmsA locus of Streptococcus pneumoniae encodes a RecG-like protein involved in DNA repair and in three-strand recombination."
      Martin B., Sharples G.J., Humbert O., Lloyd R.G., Claverys J.-P.
      Mol. Microbiol. 19:1035-1045(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 257-367.
    4. "The crystal structure of alanine racemase from Streptococcus pneumoniae, a target for structure-based drug design."
      Im H., Sharpe M.L., Strych U., Davlieva M., Krause K.L.
      BMC Microbiol. 11:116-116(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH PYRIDOXAL PHOSPHATE, COFACTOR, SUBUNIT, PYRIDOXAL PHOSPHATE AT LYS-40, CARBAMYLATION AT LYS-129.

    Entry informationi

    Entry nameiALR_STRPN
    AccessioniPrimary (citable) accession number: P0A2W8
    Secondary accession number(s): Q54899, Q9S3V7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3