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Protein

Alanine racemase

Gene

alr

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

Catalytic activityi

L-alanine = D-alanine.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation1 Publication

Pathwayi: D-alanine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-alanine from L-alanine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine racemase (alr)
This subpathway is part of the pathway D-alanine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-alanine from L-alanine, the pathway D-alanine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei40Proton acceptor; specific for D-alanineUniRule annotation1
Binding sitei136SubstrateUniRule annotation1
Active sitei263Proton acceptor; specific for L-alanineUniRule annotation1
Binding sitei310Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi5.1.1.1. 1960.
UniPathwayiUPA00042; UER00497.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
Gene namesi
Name:alr
Synonyms:alaR
Ordered Locus Names:SP_1698
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001145811 – 367Alanine racemaseAdd BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei40N6-(pyridoxal phosphate)lysine1
Modified residuei129N6-carboxylysine1 Publication1

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi170187.SpneT_02001280.

Structurei

Secondary structure

1367
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 14Combined sources5
Helixi15 – 27Combined sources13
Beta strandi34 – 38Combined sources5
Helixi40 – 44Combined sources5
Helixi48 – 55Combined sources8
Helixi56 – 58Combined sources3
Beta strandi60 – 66Combined sources7
Helixi67 – 75Combined sources9
Beta strandi82 – 87Combined sources6
Helixi90 – 92Combined sources3
Helixi93 – 98Combined sources6
Beta strandi102 – 105Combined sources4
Helixi108 – 116Combined sources9
Beta strandi125 – 130Combined sources6
Beta strandi136 – 139Combined sources4
Helixi142 – 154Combined sources13
Beta strandi158 – 164Combined sources7
Helixi175 – 189Combined sources15
Beta strandi196 – 201Combined sources6
Helixi203 – 208Combined sources6
Helixi210 – 212Combined sources3
Beta strandi215 – 219Combined sources5
Helixi221 – 224Combined sources4
Turni228 – 231Combined sources4
Beta strandi243 – 248Combined sources6
Beta strandi250 – 255Combined sources6
Beta strandi260 – 262Combined sources3
Helixi263 – 265Combined sources3
Beta strandi273 – 279Combined sources7
Helixi282 – 284Combined sources3
Helixi288 – 290Combined sources3
Beta strandi294 – 297Combined sources4
Beta strandi300 – 304Combined sources5
Beta strandi313 – 319Combined sources7
Beta strandi326 – 333Combined sources8
Beta strandi336 – 338Combined sources3
Helixi340 – 347Combined sources8
Helixi351 – 356Combined sources6
Beta strandi364 – 367Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3S46X-ray2.00A/B1-367[»]
ProteinModelPortaliP0A2W8.
SMRiP0A2W8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A2W8.

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CJ4. Bacteria.
COG0787. LUCA.
HOGENOMiHOG000031444.
KOiK01775.
OMAiPWEILCS.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase. 1 hit.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A2W8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKASPHRPTK ALIHLGAIRQ NIQQMGAHIP QGTLKLAVVK ANAYGHGAVA
60 70 80 90 100
VAKAIQDDVD GFCVSNIDEA IELRQAGLSK PILILGVSEI EAVALAKEYD
110 120 130 140 150
FTLTVAGLEW IQALLDKEVD LTGLTVHLKI DSGMGRIGFR EASEVEQAQD
160 170 180 190 200
LLQQHGVCVE GIFTHFATAD EESDDYFNAQ LERFKTILAS MKEVPELVHA
210 220 230 240 250
SNSATTLWHV ETIFNAVRMG DAMYGLNPSG AVLDLPYDLI PALTLESALV
260 270 280 290 300
HVKTVPAGAC MGYGATYQAD SEQVIATVPI GYADGWTRDM QNFSVLVDGQ
310 320 330 340 350
ACPIVGRVSM DQITIRLPKL YPLGTKVTLI GSNGDKEITA TQVATYRVTI
360
NYEVVCLLSD RIPREYY
Length:367
Mass (Da):39,858
Last modified:March 15, 2005 - v1
Checksum:iA79E2F1B439B18B7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti354V → E in CAA90279 (PubMed:8830261).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF171873 Genomic DNA. Translation: AAD51027.1.
AE005672 Genomic DNA. Translation: AAK75776.1.
Z49988 Genomic DNA. Translation: CAA90279.1.
PIRiG95197.
S71015.
RefSeqiWP_000648075.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK75776; AAK75776; SP_1698.
KEGGispn:SP_1698.
PATRICi19707839. VBIStrPne105772_1762.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF171873 Genomic DNA. Translation: AAD51027.1.
AE005672 Genomic DNA. Translation: AAK75776.1.
Z49988 Genomic DNA. Translation: CAA90279.1.
PIRiG95197.
S71015.
RefSeqiWP_000648075.1. NZ_AKVY01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3S46X-ray2.00A/B1-367[»]
ProteinModelPortaliP0A2W8.
SMRiP0A2W8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi170187.SpneT_02001280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK75776; AAK75776; SP_1698.
KEGGispn:SP_1698.
PATRICi19707839. VBIStrPne105772_1762.

Phylogenomic databases

eggNOGiENOG4105CJ4. Bacteria.
COG0787. LUCA.
HOGENOMiHOG000031444.
KOiK01775.
OMAiPWEILCS.

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
BRENDAi5.1.1.1. 1960.

Miscellaneous databases

EvolutionaryTraceiP0A2W8.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase. 1 hit.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALR_STRPN
AccessioniPrimary (citable) accession number: P0A2W8
Secondary accession number(s): Q54899, Q9S3V7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.