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Protein

cAMP-activated global transcriptional regulator CRP

Gene

crp

Organism
Klebsiella aerogenes (Enterobacter aerogenes)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A global transcription regulator. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding to regulate transcription. Induces a severe bend in DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. Plays a major role in carbon catabolite repression (CCR) (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei97Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity1
Sitei102Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi57 – 63cAMP 1By similarity7
Nucleotide bindingi72 – 74cAMP 1By similarity3
Nucleotide bindingi83 – 84cAMP 1By similarity2
Nucleotide bindingi128 – 129cAMP 1By similarity2
Nucleotide bindingi136 – 137cAMP 2By similarity2
Nucleotide bindingi171 – 181cAMP 2By similarityAdd BLAST11
DNA bindingi180 – 186H-T-H motifPROSITE-ProRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandcAMP, cAMP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-activated global transcriptional regulator CRP
Alternative name(s):
Catabolite activator protein
Short name:
CAP
Catabolite gene activator
cAMP receptor protein
Short name:
CRP
cAMP regulatory protein
Gene namesi
Name:crp
Synonyms:cap
OrganismiKlebsiella aerogenes (Enterobacter aerogenes)
Taxonomic identifieri548 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001001481 – 210cAMP-activated global transcriptional regulator CRPAdd BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei101N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Interactioni

Subunit structurei

Homodimer, which upon binding cAMP is able to bind DNA. Binds the N- and C-terminus of RNA polymerase subunit RpoA and sigma-70 (RpoD) (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP0A2T7
SMRiP0A2T7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 210HTH crp-typePROSITE-ProRule annotationAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 22Activating region 2 (AR2); probably contacts the N-terminus of RpoABy similarity3
Regioni53 – 59Activating region 3 (AR3); probably contacts sigma-70 (RpoD)By similarity7
Regioni154 – 163Activating region 1 (AR1); probably contacts the C-terminus of RpoABy similarity10

Domaini

The N-terminal domain binds cAMP and is responsible for homodimerization, while the C-terminal domain binds DNA when cAMP is bound.By similarity

Phylogenomic databases

eggNOGiCOG0664 LUCA

Family and domain databases

CDDicd00038 CAP_ED, 1 hit
Gene3Di1.10.10.10, 1 hit
2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR012318 HTH_CRP
IPR014710 RmlC-like_jellyroll
IPR018335 Tscrpt_reg_HTH_Crp-type_CS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00027 cNMP_binding, 1 hit
PF00325 Crp, 1 hit
PRINTSiPR00034 HTHCRP
SMARTiView protein in SMART
SM00100 cNMP, 1 hit
SM00419 HTH_CRP, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF51206 SSF51206, 1 hit
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 1 hit
PS00889 CNMP_BINDING_2, 1 hit
PS50042 CNMP_BINDING_3, 1 hit
PS00042 HTH_CRP_1, 1 hit
PS51063 HTH_CRP_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0A2T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLGKPQTDP TLEWFLSHCH IHKYPSKSTL IHQGEKAETL YYIVKGSVAV
60 70 80 90 100
LIKDEEGKEM ILSYLNQGDF IGELGLFEEG QERSAWVRAK TACEVAEISY
110 120 130 140 150
KKFRQLIQVN PDILMRLSSQ MARRLQVTSE KVGNLAFLDV TGRIAQTLLN
160 170 180 190 200
LAKQPDAMTH PDGMQIKITR QEIGQIVGCS RETVGRILKM LEDQNLISAH
210
GKTIVVYGTR
Length:210
Mass (Da):23,656
Last modified:January 1, 1988 - v1
Checksum:iC54244E974D53A0F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M68973 Genomic DNA Translation: AAA25058.1
RefSeqiWP_000242758.1, NZ_PQLE01000006.1

Genome annotation databases

GeneIDi7962173

Entry informationi

Entry nameiCRP_KLEAE
AccessioniPrimary (citable) accession number: P0A2T7
Secondary accession number(s): P06170, P29282
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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