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P0A2T6

- CRP_SALTY

UniProt

P0A2T6 - CRP_SALTY

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Protein
cAMP-activated global transcriptional regulator CRP
Gene
crp, cap, STM3466
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

A global transcription regulator. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding to regulate transcription. It can act as an activator, repressor, coactivator or corepressor. Induces a severe bend in DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. Plays a major role in carbon catabolite repression (CCR) By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei97 – 971Activating region 2 (AR2); probably contacts the N-terminus of RpoA By similarity
Sitei102 – 1021Activating region 2 (AR2); probably contacts the N-terminus of RpoA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi57 – 637cAMP 1 By similarity
Nucleotide bindingi72 – 743cAMP 1 By similarity
Nucleotide bindingi83 – 842cAMP 1 By similarity
Nucleotide bindingi128 – 1292cAMP 1 By similarity
Nucleotide bindingi136 – 1372cAMP 2 By similarity
Nucleotide bindingi171 – 18111cAMP 2 By similarity
Add
BLAST
DNA bindingi180 – 1867H-T-H motif By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. cAMP binding Source: UniProtKB-KW
  3. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-3488-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-activated global transcriptional regulator CRP
Alternative name(s):
Catabolite activator protein
Short name:
CAP
Catabolite gene activator
cAMP receptor protein
Short name:
CRP
cAMP regulatory protein
Gene namesi
Name:crp
Synonyms:cap
Ordered Locus Names:STM3466
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi131 – 1311K → Q or T in acr-4 and acr-3 respectively; higher levels of cAMP synthesis. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210cAMP-activated global transcriptional regulator CRP
PRO_0000100150Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei101 – 1011N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP0A2T6.
PRIDEiP0A2T6.

Interactioni

Subunit structurei

Homodimer, which upon binding cAMP is able to bind DNA. Binds the N- and C-terminus of RNA polymerase subunit RpoA and sigma-70 (RpoD) By similarity.

Protein-protein interaction databases

STRINGi99287.STM3466.

Structurei

3D structure databases

ProteinModelPortaliP0A2T6.
SMRiP0A2T6. Positions 2-208.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 21073HTH crp-type
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 223Activating region 2 (AR2); probably contacts the N-terminus of RpoA By similarity
Regioni53 – 597Activating region 3 (AR3); probably contacts sigma-70 (RpoD) By similarity
Regioni154 – 16310Activating region 1 (AR1); probably contacts the C-terminus of RpoA By similarity

Domaini

The N-terminal domain binds cAMP and is responsible for homodimerization, while the C-terminal domain binds DNA when cAMP is bound By similarity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000250565.
KOiK10914.
OMAiSETHPEF.
OrthoDBiEOG69GZGV.
PhylomeDBiP0A2T6.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A2T6-1 [UniParc]FASTAAdd to Basket

« Hide

MVLGKPQTDP TLEWFLSHCH IHKYPSKSTL IHQGEKAETL YYIVKGSVAV    50
LIKDEEGKEM ILSYLNQGDF IGELGLFEEG QERSAWVRAK TACEVAEISY 100
KKFRQLIQVN PDILMRLSSQ MARRLQVTSE KVGNLAFLDV TGRIAQTLLN 150
LAKQPDAMTH PDGMQIKITR QEIGQIVGCS RETVGRILKM LEDQNLISAH 200
GKTIVVYGTR 210
Length:210
Mass (Da):23,656
Last modified:January 1, 1988 - v1
Checksum:iC54244E974D53A0F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401L → S in AAA62414. 1 Publication
Sequence conflicti119 – 1191S → A in AAA62414. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13773 Genomic DNA. Translation: AAA27039.1.
M13770 Genomic DNA. Translation: AAA62414.1.
AE006468 Genomic DNA. Translation: AAL22328.1.
PIRiA26049.
RefSeqiNP_462369.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL22328; AAL22328; STM3466.
GeneIDi1254989.
KEGGistm:STM3466.
PATRICi32385731. VBISalEnt20916_3663.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13773 Genomic DNA. Translation: AAA27039.1 .
M13770 Genomic DNA. Translation: AAA62414.1 .
AE006468 Genomic DNA. Translation: AAL22328.1 .
PIRi A26049.
RefSeqi NP_462369.1. NC_003197.1.

3D structure databases

ProteinModelPortali P0A2T6.
SMRi P0A2T6. Positions 2-208.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM3466.

Proteomic databases

PaxDbi P0A2T6.
PRIDEi P0A2T6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL22328 ; AAL22328 ; STM3466 .
GeneIDi 1254989.
KEGGi stm:STM3466.
PATRICi 32385731. VBISalEnt20916_3663.

Phylogenomic databases

eggNOGi COG0664.
HOGENOMi HOG000250565.
KOi K10914.
OMAi SETHPEF.
OrthoDBi EOG69GZGV.
PhylomeDBi P0A2T6.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-3488-MONOMER.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProi IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view ]
PRINTSi PR00034. HTHCRP.
SMARTi SM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "crp genes of Shigella flexneri, Salmonella typhimurium, and Escherichia coli."
    Cossart P., Groisman E.A., Serre M.-C., Casadaban M.J., Gicquel-Sanzey B.
    J. Bacteriol. 167:639-646(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  2. "Cloning and DNA sequence analysis of the wild-type and mutant cyclic AMP receptor protein genes from Salmonella typhimurium."
    Schroeder C.J., Dobrogosz W.J.
    J. Bacteriol. 167:616-622(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF LYS-131.
    Strain: LT2 / SGSC1412 / ATCC 700720.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiCRP_SALTY
AccessioniPrimary (citable) accession number: P0A2T6
Secondary accession number(s): P06170, P29282
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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