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Protein

Tryptophan synthase beta chain

Gene

trpB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Bifunctional protein TrpGD (trpGD), Anthranilate synthase component 1 (trpE)
  2. Bifunctional protein TrpGD (trpGD)
  3. Tryptophan biosynthesis protein TrpCF (trpC)
  4. Tryptophan biosynthesis protein TrpCF (trpC)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • tryptophan synthase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-1743-MONOMER
UniPathwayiUPA00035; UER00044

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase beta chain (EC:4.2.1.20)
Gene namesi
Name:trpB
Ordered Locus Names:STM1726
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Chemistry databases

DrugBankiDB07748 2-({[4-(TRIFLUOROMETHOXY)PHENYL]SULFONYL}AMINO)ETHYL DIHYDROGEN PHOSPHATE
DB07732 2-[(2-NAPHTHYLSULFONYL)AMINO]ETHYL DIHYDROGEN PHOSPHATE
DB07745 2-{[4-(TRIFLUOROMETHOXY)BENZOYL]AMINO}ETHYL DIHYDROGEN PHOSPHATE
DB07894 4-(2-HYDROXY-4-FLUOROPHENYLTHIO)-BUTYLPHOSPHONIC ACID
DB07925 4-(2-HYDROXYPHENYLSULFINYL)-BUTYLPHOSPHONIC ACID
DB07890 4-(2-HYDROXYPHENYLTHIO)-1-BUTENYLPHOSPHONIC ACID
DB07773 5-FLUOROINDOLE PROPANOL PHOSPHATE
DB04143 Indole-3-Glycerol Phosphate
DB03171 Indole-3-Propanol Phosphate
DB07951 N-[1H-INDOL-3-YL-ACETYL]ASPARTIC ACID
DB07952 N-[1H-INDOL-3-YL-ACETYL]GLYCINE ACID
DB07953 N-[1H-INDOL-3-YL-ACETYL]VALINE ACID

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000989942 – 397Tryptophan synthase beta chainAdd BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei87N6-(pyridoxal phosphate)lysine1

Proteomic databases

PaxDbiP0A2K1
PRIDEiP0A2K1

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-35707N
IntActiP0A2K1, 1 interactor
MINTiP0A2K1
STRINGi99287.STM1726

Structurei

Secondary structure

1397
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 9Combined sources3
Beta strandi12 – 17Combined sources6
Helixi19 – 21Combined sources3
Helixi22 – 36Combined sources15
Helixi39 – 51Combined sources13
Turni52 – 54Combined sources3
Beta strandi59 – 61Combined sources3
Turni63 – 68Combined sources6
Beta strandi69 – 77Combined sources9
Helixi78 – 80Combined sources3
Helixi88 – 100Combined sources13
Beta strandi105 – 109Combined sources5
Beta strandi111 – 113Combined sources3
Helixi114 – 126Combined sources13
Beta strandi129 – 135Combined sources7
Helixi136 – 141Combined sources6
Helixi143 – 151Combined sources9
Beta strandi155 – 159Combined sources5
Beta strandi161 – 163Combined sources3
Helixi166 – 180Combined sources15
Turni181 – 183Combined sources3
Beta strandi184 – 186Combined sources3
Beta strandi189 – 191Combined sources3
Helixi197 – 205Combined sources9
Helixi207 – 220Combined sources14
Beta strandi225 – 230Combined sources6
Beta strandi232 – 234Combined sources3
Helixi235 – 241Combined sources7
Helixi242 – 244Combined sources3
Beta strandi250 – 259Combined sources10
Helixi262 – 264Combined sources3
Helixi270 – 273Combined sources4
Beta strandi274 – 279Combined sources6
Beta strandi281 – 286Combined sources6
Beta strandi290 – 292Combined sources3
Helixi302 – 304Combined sources3
Helixi311 – 318Combined sources8
Beta strandi321 – 328Combined sources8
Helixi329 – 343Combined sources15
Helixi349 – 364Combined sources16
Beta strandi370 – 376Combined sources7
Beta strandi378 – 380Combined sources3
Helixi381 – 383Combined sources3
Helixi384 – 390Combined sources7
Turni392 – 394Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A50X-ray2.30B2-397[»]
1A5AX-ray1.90B1-395[»]
1A5BX-ray2.00B1-395[»]
1A5SX-ray2.30B1-397[»]
1BEUX-ray1.90B1-395[»]
1BKSX-ray2.20B1-397[»]
1C29X-ray2.30B1-397[»]
1C8VX-ray2.20B1-397[»]
1C9DX-ray2.30B1-397[»]
1CW2X-ray2.00B1-397[»]
1CX9X-ray2.30B1-397[»]
1FUYX-ray2.25B2-397[»]
1K3UX-ray1.70B2-397[»]
1K7EX-ray2.30B2-397[»]
1K7FX-ray1.90B2-397[»]
1K7XX-ray1.70B2-397[»]
1K8XX-ray1.90B1-397[»]
1K8YX-ray1.50B2-397[»]
1K8ZX-ray1.70B2-397[»]
1KFBX-ray1.90B2-397[»]
1KFCX-ray1.50B1-397[»]
1KFEX-ray1.75B2-395[»]
1KFJX-ray1.80B1-397[»]
1KFKX-ray2.40B1-397[»]
1QOPX-ray1.40B2-397[»]
1QOQX-ray1.80B2-397[»]
1TJPX-ray1.50B2-397[»]
1TTPX-ray2.30B1-397[»]
1TTQX-ray2.00B1-397[»]
1UBSX-ray1.90B1-395[»]
1WBJX-ray1.50B2-397[»]
2CLEX-ray1.50B2-397[»]
2CLFX-ray1.70B2-397[»]
2CLHX-ray1.70B2-397[»]
2CLIX-ray1.70B2-397[»]
2CLKX-ray1.50B2-397[»]
2CLLX-ray1.60B2-397[»]
2CLMX-ray1.51B2-397[»]
2CLOX-ray1.50B2-397[»]
2J9XX-ray1.90B2-397[»]
2J9YX-ray1.80B1-397[»]
2J9ZX-ray1.80B1-397[»]
2RH9X-ray1.70B1-397[»]
2RHGX-ray2.00B1-397[»]
2TRSX-ray2.04B1-395[»]
2TSYX-ray2.50B1-395[»]
2TYSX-ray1.90B1-397[»]
2WSYX-ray3.05B2-397[»]
3CEPX-ray2.10B2-397[»]
3PR2X-ray1.85B3-393[»]
4HN4X-ray1.64B1-397[»]
4HPJX-ray1.45B1-397[»]
4HPXX-ray1.65B1-397[»]
4HT3X-ray1.30B1-397[»]
4KKXX-ray1.77B1-397[»]
4WX2X-ray1.75B1-397[»]
4XUGX-ray1.65B1-397[»]
4Y6GX-ray1.65B1-397[»]
4ZQCX-ray1.54B1-397[»]
5BW6X-ray1.82B1-397[»]
5CGQX-ray1.18B1-397[»]
ProteinModelPortaliP0A2K1
SMRiP0A2K1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A2K1

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpB family.Curated

Phylogenomic databases

eggNOGiENOG4105CG0 Bacteria
COG0133 LUCA
HOGENOMiHOG000161710
KOiK01696
OMAiHGMKSYF
PhylomeDBiP0A2K1

Family and domain databases

CDDicd06446 Trp-synth_B, 1 hit
HAMAPiMF_00133 Trp_synth_beta, 1 hit
InterProiView protein in InterPro
IPR001926 PLP-dep
IPR006653 Trp_synth_b_CS
IPR006654 Trp_synth_beta
IPR023026 Trp_synth_beta/beta-like
IPR036052 Trypto_synt_PLP_dependent
PANTHERiPTHR42882 PTHR42882, 1 hit
PfamiView protein in Pfam
PF00291 PALP, 1 hit
PIRSFiPIRSF001413 Trp_syn_beta, 1 hit
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR00263 trpB, 1 hit
PROSITEiView protein in PROSITE
PS00168 TRP_SYNTHASE_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A2K1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLLNPYFG EFGGMYVPQI LMPALNQLEE AFVSAQKDPE FQAQFADLLK
60 70 80 90 100
NYAGRPTALT KCQNITAGTR TTLYLKREDL LHGGAHKTNQ VLGQALLAKR
110 120 130 140 150
MGKSEIIAET GAGQHGVASA LASALLGLKC RIYMGAKDVE RQSPNVFRMR
160 170 180 190 200
LMGAEVIPVH SGSATLKDAC NEALRDWSGS YETAHYMLGT AAGPHPYPTI
210 220 230 240 250
VREFQRMIGE ETKAQILDKE GRLPDAVIAC VGGGSNAIGM FADFINDTSV
260 270 280 290 300
GLIGVEPGGH GIETGEHGAP LKHGRVGIYF GMKAPMMQTA DGQIEESYSI
310 320 330 340 350
SAGLDFPSVG PQHAYLNSIG RADYVSITDD EALEAFKTLC RHEGIIPALE
360 370 380 390
SSHALAHALK MMREQPEKEQ LLVVNLSGRG DKDIFTVHDI LKARGEI
Length:397
Mass (Da):42,868
Last modified:January 23, 2007 - v2
Checksum:i73D210F5C6F2FEBD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34S → R in CAA24667 (PubMed:7007651).Curated1
Sequence conflicti34S → R in AAA27234 (PubMed:7007651).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01377 Genomic DNA Translation: CAA24667.1
J01810 Genomic DNA Translation: AAA27234.1
AE006468 Genomic DNA Translation: AAL20644.1
V01376 Genomic DNA Translation: CAA24665.1
V00364 Genomic DNA Translation: CAA23661.1
M24299 Genomic DNA Translation: AAA99293.1
PIRiA01156 TSEBBT
RefSeqiNP_460685.1, NC_003197.2
WP_000209485.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaiAAL20644; AAL20644; STM1726
GeneIDi1253245
KEGGistm:STM1726
PATRICifig|99287.12.peg.1822

Similar proteinsi

Entry informationi

Entry nameiTRPB_SALTY
AccessioniPrimary (citable) accession number: P0A2K1
Secondary accession number(s): P00933, Q56141
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health