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Protein

Tryptophan synthase beta chain

Gene

trpB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Bifunctional protein TrpGD (trpGD), Anthranilate synthase component 1 (trpE)
  2. Bifunctional protein TrpGD (trpGD)
  3. Tryptophan biosynthesis protein TrpCF (trpC)
  4. Tryptophan biosynthesis protein TrpCF (trpC)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase beta chain (EC:4.2.1.20)
Gene namesi
Name:trpB
Ordered Locus Names:STM1726
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000989942 – 397Tryptophan synthase beta chainAdd BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei87N6-(pyridoxal phosphate)lysine1

Proteomic databases

PaxDbiP0A2K1.
PRIDEiP0A2K1.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
trpAP0092922EBI-1028431,EBI-1028423

Protein-protein interaction databases

DIPiDIP-35707N.
IntActiP0A2K1. 1 interactor.
MINTiMINT-112874.
STRINGi99287.STM1726.

Structurei

Secondary structure

1397
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 9Combined sources3
Beta strandi12 – 17Combined sources6
Helixi19 – 21Combined sources3
Helixi22 – 36Combined sources15
Helixi39 – 51Combined sources13
Turni52 – 54Combined sources3
Beta strandi59 – 61Combined sources3
Turni63 – 68Combined sources6
Beta strandi69 – 77Combined sources9
Helixi78 – 80Combined sources3
Helixi88 – 100Combined sources13
Beta strandi105 – 109Combined sources5
Beta strandi111 – 113Combined sources3
Helixi114 – 126Combined sources13
Beta strandi129 – 135Combined sources7
Helixi136 – 141Combined sources6
Helixi143 – 151Combined sources9
Beta strandi155 – 159Combined sources5
Beta strandi161 – 163Combined sources3
Helixi166 – 180Combined sources15
Turni181 – 183Combined sources3
Beta strandi184 – 186Combined sources3
Beta strandi189 – 191Combined sources3
Helixi197 – 205Combined sources9
Helixi207 – 220Combined sources14
Beta strandi225 – 230Combined sources6
Beta strandi232 – 234Combined sources3
Helixi235 – 241Combined sources7
Helixi242 – 244Combined sources3
Beta strandi250 – 259Combined sources10
Helixi262 – 264Combined sources3
Helixi270 – 273Combined sources4
Beta strandi274 – 279Combined sources6
Beta strandi281 – 286Combined sources6
Beta strandi290 – 292Combined sources3
Helixi302 – 304Combined sources3
Helixi311 – 318Combined sources8
Beta strandi321 – 328Combined sources8
Helixi329 – 343Combined sources15
Helixi349 – 364Combined sources16
Beta strandi370 – 376Combined sources7
Beta strandi378 – 380Combined sources3
Helixi381 – 383Combined sources3
Helixi384 – 390Combined sources7
Turni392 – 394Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A50X-ray2.30B2-397[»]
1A5AX-ray1.90B1-395[»]
1A5BX-ray2.00B1-395[»]
1A5SX-ray2.30B1-397[»]
1BEUX-ray1.90B1-395[»]
1BKSX-ray2.20B1-397[»]
1C29X-ray2.30B1-397[»]
1C8VX-ray2.20B1-397[»]
1C9DX-ray2.30B1-397[»]
1CW2X-ray2.00B1-397[»]
1CX9X-ray2.30B1-397[»]
1FUYX-ray2.25B2-397[»]
1K3UX-ray1.70B2-397[»]
1K7EX-ray2.30B2-397[»]
1K7FX-ray1.90B2-397[»]
1K7XX-ray1.70B2-397[»]
1K8XX-ray1.90B1-397[»]
1K8YX-ray1.50B2-397[»]
1K8ZX-ray1.70B2-397[»]
1KFBX-ray1.90B2-397[»]
1KFCX-ray1.50B1-397[»]
1KFEX-ray1.75B2-395[»]
1KFJX-ray1.80B1-397[»]
1KFKX-ray2.40B1-397[»]
1QOPX-ray1.40B2-397[»]
1QOQX-ray1.80B2-397[»]
1TJPX-ray1.50B2-397[»]
1TTPX-ray2.30B1-397[»]
1TTQX-ray2.00B1-397[»]
1UBSX-ray1.90B1-395[»]
1WBJX-ray1.50B2-397[»]
2CLEX-ray1.50B2-397[»]
2CLFX-ray1.70B2-397[»]
2CLHX-ray1.70B2-397[»]
2CLIX-ray1.70B2-397[»]
2CLKX-ray1.50B2-397[»]
2CLLX-ray1.60B2-397[»]
2CLMX-ray1.51B2-397[»]
2CLOX-ray1.50B2-397[»]
2J9XX-ray1.90B2-397[»]
2J9YX-ray1.80B1-397[»]
2J9ZX-ray1.80B1-397[»]
2RH9X-ray1.70B1-397[»]
2RHGX-ray2.00B1-397[»]
2TRSX-ray2.04B1-395[»]
2TSYX-ray2.50B1-395[»]
2TYSX-ray1.90B1-397[»]
2WSYX-ray3.05B2-397[»]
3CEPX-ray2.10B2-397[»]
3PR2X-ray1.85B3-393[»]
4HN4X-ray1.64B1-397[»]
4HPJX-ray1.45B1-397[»]
4HPXX-ray1.65B1-397[»]
4HT3X-ray1.30B1-397[»]
4KKXX-ray1.77B1-397[»]
4WX2X-ray1.75B1-397[»]
4XUGX-ray1.65B1-397[»]
4Y6GX-ray1.65B1-397[»]
4ZQCX-ray1.54B1-397[»]
5BW6X-ray1.82B1-397[»]
5CGQX-ray1.18B1-397[»]
ProteinModelPortaliP0A2K1.
SMRiP0A2K1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A2K1.

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpB family.Curated

Phylogenomic databases

eggNOGiENOG4105CG0. Bacteria.
COG0133. LUCA.
HOGENOMiHOG000161710.
KOiK01696.
OMAiCQKEGII.
PhylomeDBiP0A2K1.

Family and domain databases

CDDicd06446. Trp-synth_B. 1 hit.
HAMAPiMF_00133. Trp_synth_beta. 1 hit.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A2K1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLLNPYFG EFGGMYVPQI LMPALNQLEE AFVSAQKDPE FQAQFADLLK
60 70 80 90 100
NYAGRPTALT KCQNITAGTR TTLYLKREDL LHGGAHKTNQ VLGQALLAKR
110 120 130 140 150
MGKSEIIAET GAGQHGVASA LASALLGLKC RIYMGAKDVE RQSPNVFRMR
160 170 180 190 200
LMGAEVIPVH SGSATLKDAC NEALRDWSGS YETAHYMLGT AAGPHPYPTI
210 220 230 240 250
VREFQRMIGE ETKAQILDKE GRLPDAVIAC VGGGSNAIGM FADFINDTSV
260 270 280 290 300
GLIGVEPGGH GIETGEHGAP LKHGRVGIYF GMKAPMMQTA DGQIEESYSI
310 320 330 340 350
SAGLDFPSVG PQHAYLNSIG RADYVSITDD EALEAFKTLC RHEGIIPALE
360 370 380 390
SSHALAHALK MMREQPEKEQ LLVVNLSGRG DKDIFTVHDI LKARGEI
Length:397
Mass (Da):42,868
Last modified:January 23, 2007 - v2
Checksum:i73D210F5C6F2FEBD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34S → R in CAA24667 (PubMed:7007651).Curated1
Sequence conflicti34S → R in AAA27234 (PubMed:7007651).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01377 Genomic DNA. Translation: CAA24667.1.
J01810 Genomic DNA. Translation: AAA27234.1.
AE006468 Genomic DNA. Translation: AAL20644.1.
V01376 Genomic DNA. Translation: CAA24665.1.
V00364 Genomic DNA. Translation: CAA23661.1.
M24299 Genomic DNA. Translation: AAA99293.1.
PIRiA01156. TSEBBT.
RefSeqiNP_460685.1. NC_003197.1.
WP_000209485.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20644; AAL20644; STM1726.
GeneIDi1253245.
KEGGistm:STM1726.
PATRICi32381965. VBISalEnt20916_1822.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01377 Genomic DNA. Translation: CAA24667.1.
J01810 Genomic DNA. Translation: AAA27234.1.
AE006468 Genomic DNA. Translation: AAL20644.1.
V01376 Genomic DNA. Translation: CAA24665.1.
V00364 Genomic DNA. Translation: CAA23661.1.
M24299 Genomic DNA. Translation: AAA99293.1.
PIRiA01156. TSEBBT.
RefSeqiNP_460685.1. NC_003197.1.
WP_000209485.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A50X-ray2.30B2-397[»]
1A5AX-ray1.90B1-395[»]
1A5BX-ray2.00B1-395[»]
1A5SX-ray2.30B1-397[»]
1BEUX-ray1.90B1-395[»]
1BKSX-ray2.20B1-397[»]
1C29X-ray2.30B1-397[»]
1C8VX-ray2.20B1-397[»]
1C9DX-ray2.30B1-397[»]
1CW2X-ray2.00B1-397[»]
1CX9X-ray2.30B1-397[»]
1FUYX-ray2.25B2-397[»]
1K3UX-ray1.70B2-397[»]
1K7EX-ray2.30B2-397[»]
1K7FX-ray1.90B2-397[»]
1K7XX-ray1.70B2-397[»]
1K8XX-ray1.90B1-397[»]
1K8YX-ray1.50B2-397[»]
1K8ZX-ray1.70B2-397[»]
1KFBX-ray1.90B2-397[»]
1KFCX-ray1.50B1-397[»]
1KFEX-ray1.75B2-395[»]
1KFJX-ray1.80B1-397[»]
1KFKX-ray2.40B1-397[»]
1QOPX-ray1.40B2-397[»]
1QOQX-ray1.80B2-397[»]
1TJPX-ray1.50B2-397[»]
1TTPX-ray2.30B1-397[»]
1TTQX-ray2.00B1-397[»]
1UBSX-ray1.90B1-395[»]
1WBJX-ray1.50B2-397[»]
2CLEX-ray1.50B2-397[»]
2CLFX-ray1.70B2-397[»]
2CLHX-ray1.70B2-397[»]
2CLIX-ray1.70B2-397[»]
2CLKX-ray1.50B2-397[»]
2CLLX-ray1.60B2-397[»]
2CLMX-ray1.51B2-397[»]
2CLOX-ray1.50B2-397[»]
2J9XX-ray1.90B2-397[»]
2J9YX-ray1.80B1-397[»]
2J9ZX-ray1.80B1-397[»]
2RH9X-ray1.70B1-397[»]
2RHGX-ray2.00B1-397[»]
2TRSX-ray2.04B1-395[»]
2TSYX-ray2.50B1-395[»]
2TYSX-ray1.90B1-397[»]
2WSYX-ray3.05B2-397[»]
3CEPX-ray2.10B2-397[»]
3PR2X-ray1.85B3-393[»]
4HN4X-ray1.64B1-397[»]
4HPJX-ray1.45B1-397[»]
4HPXX-ray1.65B1-397[»]
4HT3X-ray1.30B1-397[»]
4KKXX-ray1.77B1-397[»]
4WX2X-ray1.75B1-397[»]
4XUGX-ray1.65B1-397[»]
4Y6GX-ray1.65B1-397[»]
4ZQCX-ray1.54B1-397[»]
5BW6X-ray1.82B1-397[»]
5CGQX-ray1.18B1-397[»]
ProteinModelPortaliP0A2K1.
SMRiP0A2K1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-35707N.
IntActiP0A2K1. 1 interactor.
MINTiMINT-112874.
STRINGi99287.STM1726.

Proteomic databases

PaxDbiP0A2K1.
PRIDEiP0A2K1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20644; AAL20644; STM1726.
GeneIDi1253245.
KEGGistm:STM1726.
PATRICi32381965. VBISalEnt20916_1822.

Phylogenomic databases

eggNOGiENOG4105CG0. Bacteria.
COG0133. LUCA.
HOGENOMiHOG000161710.
KOiK01696.
OMAiCQKEGII.
PhylomeDBiP0A2K1.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.

Miscellaneous databases

EvolutionaryTraceiP0A2K1.

Family and domain databases

CDDicd06446. Trp-synth_B. 1 hit.
HAMAPiMF_00133. Trp_synth_beta. 1 hit.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPB_SALTY
AccessioniPrimary (citable) accession number: P0A2K1
Secondary accession number(s): P00933, Q56141
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.