Reviewed, UniProtKB/Swiss-Prot P0A2I2 (TOP1_SALTI)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	STY1336, t1627

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	865 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 865	865	DNA topoisomerase 1		PRO_0000145164
Regions
	Zinc finger	599 – 630	32	C4-type 1
	Zinc finger	662 – 689	28	C4-type 2
	Zinc finger	711 – 736	26	C4-type 3
Sites
	Active site	319	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A2I2-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 57EE1CBA696FEF7C	FASTA	865	97,303
	10 20 30 40 50 60 MGKALVIVES PAKAKTINKY LGNDYVVKSS VGHIRDLPTS GSAAKKSADS TSTKTAKKPK 70 80 90 100 110 120 KDERGALVNR MGVDPWHNWD AHYEVLPGKE KVVSELKQLA EKADHIYLAT DLDREGEAIA 130 140 150 160 170 180 WHLREVIGGD DARYSRVVFN EITKNAIRQA FEQPGELNIN RVNAQQARRF MDRVVGYMVS 190 200 210 220 230 240 PLLWKKIARG LSAGRVQSVA VRLVVERERE IKAFVPEEFW EIDANTTTPS GEALPLQVTH 250 260 270 280 290 300 QNDKPFRPVN REQTLAAVSL LEKARYSVLE REDKPTSSKP GAPFITSTLQ QAASTRLGFG 310 320 330 340 350 360 VKKTMMMAQR LYEAGYITYM RTDSTNLSQD AVNMVRGYIG DNFGKKYLPD NPNQYASKEN 370 380 390 400 410 420 SQEAHEAIRP SDVAVMAESL KDMEADAQKL YQLIWRQFVA CQMTPAQYDS TTLTVGAGEF 430 440 450 460 470 480 RLKARGRILR FDGWTKVMPA LRKGDEDRTL PAVNKGDALT LLELTPAQHF TKPPARFSEA 490 500 510 520 530 540 SLVKELEKRG IGRPSTYASI ISTIQDRGYV RVENRRFYAE KMGEIVTDRL EENFRELMNY 550 560 570 580 590 600 DFTAQMEDSL DQVANHQAEW KAVLDNFFSD FTQQLDKAEK DPEEGGMRPN QMVLTSIDCP 610 620 630 640 650 660 TCGRKMGIRT ASTGVFLGCS GYALSPKERC KTTINLVPEN EVLNVLEGDD AETNALRAKR 670 680 690 700 710 720 RCQKCGTAMD SYLIDPKRKL HVCGNNPTCD GYEIEEGEFR IKGYDGPIVE CEKCGSEMHL 730 740 750 760 770 780 KMGRFGKYMA CTNDECKNTR KILRNGEVAP PKEDPVPLPE LPCEKSDAYF VLRDGAAGIF 790 800 810 820 830 840 LAANTFPKSR ETRAPLVEEL YRFRDRLPEK LRYLADAPQQ DPEGNKTVVR FSRKTKQQYV 850 860 AAEKDGKATG WSAFFVDGKW VEGKK

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL627269 Genomic DNA. Translation: CAD08417.1. AE014613 Genomic DNA. Translation: AAO69254.1.
RefSeq	NP_455783.1. NP_805405.1.
3D structure databases
HSSP	HSSP built from PDB template 1CY8 based on UniProtKB P06612.
SMR	P0A2I2. Positions 2-592.
ModBase	Search...
Proteomic databases
PRIDE	P0A2I2.
Genome annotation databases
GeneID	1071184. 1247736.
GenomeReviews	Gene locus t1627 in contig AE014613_GR. Gene locus STY1336 in contig AL513382_GR.
KEGG	stt:t1627. sty:STY1336.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A2I2.
OMA	RKFYGCS.
Enzyme and pathway databases
BioCyc	SENT209261:T1627-MON. SENT220341:STY1336-MON.
BRENDA	5.99.1.2. 3716.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac. IPR013263. TopoI_Znr_bac. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF08272. Topo_Zn_Ribbon. 2 hits. PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_SALTI

Accession

Primary (citable) accession number: P0A2I2
Secondary accession number(s): P40686

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)