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P0A2I1

- TOP1_SALTY

UniProt

P0A2I1 - TOP1_SALTY

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Protein

DNA topoisomerase 1

Gene

topA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
Sitei33 – 331Interaction with DNAUniRule annotation
Metal bindingi111 – 1111Magnesium 1; catalyticUniRule annotation
Metal bindingi111 – 1111Magnesium 2UniRule annotation
Metal bindingi113 – 1131Magnesium 2UniRule annotation
Sitei168 – 1681Interaction with DNAUniRule annotation
Sitei169 – 1691Interaction with DNAUniRule annotation
Sitei172 – 1721Interaction with DNAUniRule annotation
Sitei177 – 1771Interaction with DNAUniRule annotation
Sitei184 – 1841Interaction with DNAUniRule annotation
Active sitei319 – 3191O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei321 – 3211Interaction with DNAUniRule annotation
Sitei507 – 5071Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri599 – 63032C4-type 1Add
BLAST
Zinc fingeri662 – 68928C4-type 2Add
BLAST
Zinc fingeri711 – 73626C4-type 3Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSENT99287:GCTI-1723-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:STM1714
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 865865DNA topoisomerase 1PRO_0000145165Add
BLAST

Proteomic databases

PaxDbiP0A2I1.
PRIDEiP0A2I1.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi99287.STM1714.

Structurei

3D structure databases

ProteinModelPortaliP0A2I1.
SMRiP0A2I1. Positions 2-595, 745-865.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 142140ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni192 – 1976Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri599 – 63032C4-type 1Add
BLAST
Zinc fingeri662 – 68928C4-type 2Add
BLAST
Zinc fingeri711 – 73626C4-type 3Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.
PhylomeDBiP0A2I1.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A2I1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKALVIVES PAKAKTINKY LGNDYVVKSS VGHIRDLPTS GSAAKKSADS
60 70 80 90 100
TSTKTAKKPK KDERGALVNR MGVDPWHNWD AHYEVLPGKE KVVSELKQLA
110 120 130 140 150
EKADHIYLAT DLDREGEAIA WHLREVIGGD DARYSRVVFN EITKNAIRQA
160 170 180 190 200
FEQPGELNIN RVNAQQARRF MDRVVGYMVS PLLWKKIARG LSAGRVQSVA
210 220 230 240 250
VRLVVERERE IKAFVPEEFW EIDANTTTPS GEALPLQVTH QNDKPFRPVN
260 270 280 290 300
REQTLAAVSL LEKARYSVLE REDKPTSSKP GAPFITSTLQ QAASTRLGFG
310 320 330 340 350
VKKTMMMAQR LYEAGYITYM RTDSTNLSQD AVNMVRGYIG DNFGKKYLPD
360 370 380 390 400
NPNQYASKEN SQEAHEAIRP SDVAVMAESL KDMEADAQKL YQLIWRQFVA
410 420 430 440 450
CQMTPAQYDS TTLTVGAGEF RLKARGRILR FDGWTKVMPA LRKGDEDRTL
460 470 480 490 500
PAVNKGDALT LLELTPAQHF TKPPARFSEA SLVKELEKRG IGRPSTYASI
510 520 530 540 550
ISTIQDRGYV RVENRRFYAE KMGEIVTDRL EENFRELMNY DFTAQMEDSL
560 570 580 590 600
DQVANHQAEW KAVLDNFFSD FTQQLDKAEK DPEEGGMRPN QMVLTSIDCP
610 620 630 640 650
TCGRKMGIRT ASTGVFLGCS GYALSPKERC KTTINLVPEN EVLNVLEGDD
660 670 680 690 700
AETNALRAKR RCQKCGTAMD SYLIDPKRKL HVCGNNPTCD GYEIEEGEFR
710 720 730 740 750
IKGYDGPIVE CEKCGSEMHL KMGRFGKYMA CTNDECKNTR KILRNGEVAP
760 770 780 790 800
PKEDPVPLPE LPCEKSDAYF VLRDGAAGIF LAANTFPKSR ETRAPLVEEL
810 820 830 840 850
YRFRDRLPEK LRYLADAPQQ DPEGNKTVVR FSRKTKQQYV AAEKDGKATG
860
WSAFFVDGKW VEGKK
Length:865
Mass (Da):97,303
Last modified:March 15, 2005 - v1
Checksum:i57EE1CBA696FEF7C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL20632.1.
M15040 Genomic DNA. Translation: AAA27044.1.
RefSeqiNP_460673.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20632; AAL20632; STM1714.
GeneIDi1253233.
KEGGistm:STM1714.
PATRICi32381943. VBISalEnt20916_1811.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL20632.1 .
M15040 Genomic DNA. Translation: AAA27044.1 .
RefSeqi NP_460673.1. NC_003197.1.

3D structure databases

ProteinModelPortali P0A2I1.
SMRi P0A2I1. Positions 2-595, 745-865.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM1714.

Proteomic databases

PaxDbi P0A2I1.
PRIDEi P0A2I1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL20632 ; AAL20632 ; STM1714 .
GeneIDi 1253233.
KEGGi stm:STM1714.
PATRICi 32381943. VBISalEnt20916_1811.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000004018.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.
PhylomeDBi P0A2I1.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-1723-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  2. "DNA sequences of the cysB regions of Salmonella typhimurium and Escherichia coli."
    Ostrowski J., Jagura-Burdzy G., Kredich N.M.
    J. Biol. Chem. 262:5999-6005(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 856-865.

Entry informationi

Entry nameiTOP1_SALTY
AccessioniPrimary (citable) accession number: P0A2I1
Secondary accession number(s): P40686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3