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P0A2I1

- TOP1_SALTY

UniProt

P0A2I1 - TOP1_SALTY

Protein

DNA topoisomerase 1

Gene

topA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
    Sitei33 – 331Interaction with DNAUniRule annotation
    Metal bindingi111 – 1111Magnesium 1; catalyticUniRule annotation
    Metal bindingi111 – 1111Magnesium 2UniRule annotation
    Metal bindingi113 – 1131Magnesium 2UniRule annotation
    Sitei168 – 1681Interaction with DNAUniRule annotation
    Sitei169 – 1691Interaction with DNAUniRule annotation
    Sitei172 – 1721Interaction with DNAUniRule annotation
    Sitei177 – 1771Interaction with DNAUniRule annotation
    Sitei184 – 1841Interaction with DNAUniRule annotation
    Active sitei319 – 3191O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei321 – 3211Interaction with DNAUniRule annotation
    Sitei507 – 5071Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri599 – 63032C4-type 1Add
    BLAST
    Zinc fingeri662 – 68928C4-type 2Add
    BLAST
    Zinc fingeri711 – 73626C4-type 3Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
    4. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-1723-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:STM1714
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 865865DNA topoisomerase 1PRO_0000145165Add
    BLAST

    Proteomic databases

    PaxDbiP0A2I1.
    PRIDEiP0A2I1.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi99287.STM1714.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A2I1.
    SMRiP0A2I1. Positions 2-595, 745-865.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 142140ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni192 – 1976Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri599 – 63032C4-type 1Add
    BLAST
    Zinc fingeri662 – 68928C4-type 2Add
    BLAST
    Zinc fingeri711 – 73626C4-type 3Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0551.
    HOGENOMiHOG000004018.
    KOiK03168.
    OMAiVVECDKC.
    OrthoDBiEOG6S7XQ9.
    PhylomeDBiP0A2I1.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR013263. TopoI_Znr_bac.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF08272. Topo_Zn_Ribbon. 2 hits.
    PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A2I1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKALVIVES PAKAKTINKY LGNDYVVKSS VGHIRDLPTS GSAAKKSADS    50
    TSTKTAKKPK KDERGALVNR MGVDPWHNWD AHYEVLPGKE KVVSELKQLA 100
    EKADHIYLAT DLDREGEAIA WHLREVIGGD DARYSRVVFN EITKNAIRQA 150
    FEQPGELNIN RVNAQQARRF MDRVVGYMVS PLLWKKIARG LSAGRVQSVA 200
    VRLVVERERE IKAFVPEEFW EIDANTTTPS GEALPLQVTH QNDKPFRPVN 250
    REQTLAAVSL LEKARYSVLE REDKPTSSKP GAPFITSTLQ QAASTRLGFG 300
    VKKTMMMAQR LYEAGYITYM RTDSTNLSQD AVNMVRGYIG DNFGKKYLPD 350
    NPNQYASKEN SQEAHEAIRP SDVAVMAESL KDMEADAQKL YQLIWRQFVA 400
    CQMTPAQYDS TTLTVGAGEF RLKARGRILR FDGWTKVMPA LRKGDEDRTL 450
    PAVNKGDALT LLELTPAQHF TKPPARFSEA SLVKELEKRG IGRPSTYASI 500
    ISTIQDRGYV RVENRRFYAE KMGEIVTDRL EENFRELMNY DFTAQMEDSL 550
    DQVANHQAEW KAVLDNFFSD FTQQLDKAEK DPEEGGMRPN QMVLTSIDCP 600
    TCGRKMGIRT ASTGVFLGCS GYALSPKERC KTTINLVPEN EVLNVLEGDD 650
    AETNALRAKR RCQKCGTAMD SYLIDPKRKL HVCGNNPTCD GYEIEEGEFR 700
    IKGYDGPIVE CEKCGSEMHL KMGRFGKYMA CTNDECKNTR KILRNGEVAP 750
    PKEDPVPLPE LPCEKSDAYF VLRDGAAGIF LAANTFPKSR ETRAPLVEEL 800
    YRFRDRLPEK LRYLADAPQQ DPEGNKTVVR FSRKTKQQYV AAEKDGKATG 850
    WSAFFVDGKW VEGKK 865
    Length:865
    Mass (Da):97,303
    Last modified:March 15, 2005 - v1
    Checksum:i57EE1CBA696FEF7C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL20632.1.
    M15040 Genomic DNA. Translation: AAA27044.1.
    RefSeqiNP_460673.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL20632; AAL20632; STM1714.
    GeneIDi1253233.
    KEGGistm:STM1714.
    PATRICi32381943. VBISalEnt20916_1811.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL20632.1 .
    M15040 Genomic DNA. Translation: AAA27044.1 .
    RefSeqi NP_460673.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali P0A2I1.
    SMRi P0A2I1. Positions 2-595, 745-865.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM1714.

    Proteomic databases

    PaxDbi P0A2I1.
    PRIDEi P0A2I1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL20632 ; AAL20632 ; STM1714 .
    GeneIDi 1253233.
    KEGGi stm:STM1714.
    PATRICi 32381943. VBISalEnt20916_1811.

    Phylogenomic databases

    eggNOGi COG0551.
    HOGENOMi HOG000004018.
    KOi K03168.
    OMAi VVECDKC.
    OrthoDBi EOG6S7XQ9.
    PhylomeDBi P0A2I1.

    Enzyme and pathway databases

    BioCyci SENT99287:GCTI-1723-MONOMER.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR013263. TopoI_Znr_bac.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF08272. Topo_Zn_Ribbon. 2 hits.
    PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    2. "DNA sequences of the cysB regions of Salmonella typhimurium and Escherichia coli."
      Ostrowski J., Jagura-Burdzy G., Kredich N.M.
      J. Biol. Chem. 262:5999-6005(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 856-865.

    Entry informationi

    Entry nameiTOP1_SALTY
    AccessioniPrimary (citable) accession number: P0A2I1
    Secondary accession number(s): P40686
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3