P0A2I1 (TOP1_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme

Gene names

Name:	topA
Ordered Locus Names:	STM1714

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	865 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 865	865	DNA topoisomerase 1		PRO_0000145165
Regions
	Domain	3 – 140	138	Toprim
	Zinc finger	599 – 630	32	C4-type 1
	Zinc finger	662 – 689	28	C4-type 2
	Zinc finger	711 – 736	26	C4-type 3
Sites
	Active site	319	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A2I1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 57EE1CBA696FEF7C	FASTA	865	97,303
	10 20 30 40 50 60 MGKALVIVES PAKAKTINKY LGNDYVVKSS VGHIRDLPTS GSAAKKSADS TSTKTAKKPK 70 80 90 100 110 120 KDERGALVNR MGVDPWHNWD AHYEVLPGKE KVVSELKQLA EKADHIYLAT DLDREGEAIA 130 140 150 160 170 180 WHLREVIGGD DARYSRVVFN EITKNAIRQA FEQPGELNIN RVNAQQARRF MDRVVGYMVS 190 200 210 220 230 240 PLLWKKIARG LSAGRVQSVA VRLVVERERE IKAFVPEEFW EIDANTTTPS GEALPLQVTH 250 260 270 280 290 300 QNDKPFRPVN REQTLAAVSL LEKARYSVLE REDKPTSSKP GAPFITSTLQ QAASTRLGFG 310 320 330 340 350 360 VKKTMMMAQR LYEAGYITYM RTDSTNLSQD AVNMVRGYIG DNFGKKYLPD NPNQYASKEN 370 380 390 400 410 420 SQEAHEAIRP SDVAVMAESL KDMEADAQKL YQLIWRQFVA CQMTPAQYDS TTLTVGAGEF 430 440 450 460 470 480 RLKARGRILR FDGWTKVMPA LRKGDEDRTL PAVNKGDALT LLELTPAQHF TKPPARFSEA 490 500 510 520 530 540 SLVKELEKRG IGRPSTYASI ISTIQDRGYV RVENRRFYAE KMGEIVTDRL EENFRELMNY 550 560 570 580 590 600 DFTAQMEDSL DQVANHQAEW KAVLDNFFSD FTQQLDKAEK DPEEGGMRPN QMVLTSIDCP 610 620 630 640 650 660 TCGRKMGIRT ASTGVFLGCS GYALSPKERC KTTINLVPEN EVLNVLEGDD AETNALRAKR 670 680 690 700 710 720 RCQKCGTAMD SYLIDPKRKL HVCGNNPTCD GYEIEEGEFR IKGYDGPIVE CEKCGSEMHL 730 740 750 760 770 780 KMGRFGKYMA CTNDECKNTR KILRNGEVAP PKEDPVPLPE LPCEKSDAYF VLRDGAAGIF 790 800 810 820 830 840 LAANTFPKSR ETRAPLVEEL YRFRDRLPEK LRYLADAPQQ DPEGNKTVVR FSRKTKQQYV 850 860 AAEKDGKATG WSAFFVDGKW VEGKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[2]	"DNA sequences of the cysB regions of Salmonella typhimurium and Escherichia coli." Ostrowski J., Jagura-Burdzy G., Kredich N.M. J. Biol. Chem. 262:5999-6005(1987) [PubMed: 3032952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 856-865.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006468 Genomic DNA. Translation: AAL20632.1. M15040 Genomic DNA. Translation: AAA27044.1.
RefSeq	NP_460673.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P0A2I1.
SMR	P0A2I1. Positions 2-595, 745-865.
ModBase	Search...
Proteomic databases
PRIDE	P0A2I1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1253233.
GenomeReviews	Gene locus STM1714 in contig AE006468_GR.
KEGG	stm:STM1714.
PATRIC	32381943. VBISalEnt20916_1811.
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK07561.
Enzyme and pathway databases
BioCyc	STYP99287:STM1714-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR013263. TopoI_Znr_bac. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF08272. Topo_Zn_Ribbon. 2 hits. PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_SALTY

Accession

Primary (citable) accession number: P0A2I1
Secondary accession number(s): P40686

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program