P0A2D0 (FABG_SALTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG
EC=1.1.1.100

Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase

Gene names

Name:	fabG
Ordered Locus Names:	STY1234, t1725

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis By similarity.
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Miscellaneous	Calcium ions stabilize the structure, and may inhibit FabG activity by obstructing access to the active site By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Ligand	Calcium Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	fatty acid elongation Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Inferred from sequence or structural similarity. Source: UniProtKB NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	3-oxoacyl-[acyl-carrier-protein] reductase FabG		PRO_0000054680
Regions
	Nucleotide binding	12 – 15	4	NADP By similarity
	Nucleotide binding	59 – 60	2	NADP By similarity
	Nucleotide binding	151 – 155	5	NADP By similarity
Sites
	Active site	151	1	Proton acceptor By similarity
	Metal binding	50	1	Calcium 1; via carbonyl oxygen; shared with dimeric partner By similarity
	Metal binding	53	1	Calcium 1; via carbonyl oxygen; shared with dimeric partner By similarity
	Metal binding	145	1	Calcium 2 By similarity
	Metal binding	233	1	Calcium 3; shared with dimeric partner By similarity
	Metal binding	234	1	Calcium 3; via carbonyl oxygen; shared with dimeric partner By similarity
	Binding site	37	1	NADP By similarity
	Binding site	86	1	NADP; via carbonyl oxygen By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	184	1	NADP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A2D0 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 86D71DA3E9AF0363	FASTA	244	25,545
	10 20 30 40 50 60 MSFEGKIALV TGASRGIGRA IAETLVARGA KVIGTATSEN GAKNISDYLG ANGKGLMLNV 70 80 90 100 110 120 TDPASIESVL ENIRAEFGEV DILVNNAGIT RDNLLMRMKD DEWNDIIETN LSSVFRLSKA 130 140 150 160 170 180 VMRAMMKKRC GRIITIGSVV GTMGNAGQAN YAAAKAGLIG FSKSLAREVA SRGITVNVVA 190 200 210 220 230 240 PGFIETDMTR ALSDDQRAGI LAQVPAGRLG GAQEIASAVA FLASDEASYI TGETLHVNGG MYMV

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL627269 Genomic DNA. Translation: CAD08319.1. AE014613 Genomic DNA. Translation: AAO69349.1.
RefSeq	NP_805500.1. NC_004631.1.
3D structure databases
ProteinModelPortal	P0A2D0.
SMR	P0A2D0. Positions 2-244.
ModBase	Search...
Protein-protein interaction databases
STRING	220341.STY1234.
Proteomic databases
PRIDE	P0A2D0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO69349; AAO69349; t1725. CAD08319; CAD08319; CAD08319.
GeneID	1071364.
KEGG	stt:t1725.
PATRIC	18540341. VBISalEnt120419_1236.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1028.
KO	K00059.
OMA	MSKAVMR.
ProtClustDB	PRK05557.
Enzyme and pathway databases
UniPathway	UPA00094.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR011284. 3oxo_ACP_reduc. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FABG_SALTI

Accession

Primary (citable) accession number: P0A2D0
Secondary accession number(s): O85141

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program