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Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.1 Publication

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37NADPBy similarity1
Metal bindingi50Calcium 1; via carbonyl oxygen; shared with dimeric partnerBy similarity1
Metal bindingi53Calcium 1; via carbonyl oxygen; shared with dimeric partnerBy similarity1
Binding sitei86NADP; via carbonyl oxygenBy similarity1
Binding sitei138SubstrateBy similarity1
Metal bindingi145Calcium 2By similarity1
Active sitei151Proton acceptorPROSITE-ProRule annotation1
Binding sitei184NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Metal bindingi233Calcium 3; shared with dimeric partnerBy similarity1
Metal bindingi234Calcium 3; via carbonyl oxygen; shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 15NADPBy similarity4
Nucleotide bindingi59 – 60NADPBy similarity2
Nucleotide bindingi151 – 155NADPBy similarity5

GO - Molecular functioni

GO - Biological processi

  • fatty acid elongation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:STM1195
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi125M → I: Loss of the temperature-sensitive phenotype; when associated with T-223. 1 Publication1
Mutagenesisi223A → T: Loss of the temperature-sensitive phenotype; when associated with I-125. 1 Publication1
Mutagenesisi224S → F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000546811 – 2443-oxoacyl-[acyl-carrier-protein] reductase FabGAdd BLAST244

Proteomic databases

PaxDbiP0A2C9.
PRIDEiP0A2C9.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi99287.STM1195.

Structurei

3D structure databases

ProteinModelPortaliP0A2C9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
KOiK00059.
OMAiMKDEDWN.
PhylomeDBiP0A2C9.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A2C9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFEGKIALV TGASRGIGRA IAETLVARGA KVIGTATSEN GAKNISDYLG
60 70 80 90 100
ANGKGLMLNV TDPASIESVL ENIRAEFGEV DILVNNAGIT RDNLLMRMKD
110 120 130 140 150
DEWNDIIETN LSSVFRLSKA VMRAMMKKRC GRIITIGSVV GTMGNAGQAN
160 170 180 190 200
YAAAKAGLIG FSKSLAREVA SRGITVNVVA PGFIETDMTR ALSDDQRAGI
210 220 230 240
LAQVPAGRLG GAQEIASAVA FLASDEASYI TGETLHVNGG MYMV
Length:244
Mass (Da):25,545
Last modified:March 15, 2005 - v1
Checksum:i86D71DA3E9AF0363
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044668 Genomic DNA. Translation: AAC38650.1.
AE006468 Genomic DNA. Translation: AAL20124.1.
RefSeqiNP_460165.1. NC_003197.1.
WP_000007236.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20124; AAL20124; STM1195.
GeneIDi1252713.
KEGGistm:STM1195.
PATRICi32380839. VBISalEnt20916_1264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044668 Genomic DNA. Translation: AAC38650.1.
AE006468 Genomic DNA. Translation: AAL20124.1.
RefSeqiNP_460165.1. NC_003197.1.
WP_000007236.1. NC_003197.1.

3D structure databases

ProteinModelPortaliP0A2C9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1195.

Proteomic databases

PaxDbiP0A2C9.
PRIDEiP0A2C9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20124; AAL20124; STM1195.
GeneIDi1252713.
KEGGistm:STM1195.
PATRICi32380839. VBISalEnt20916_1264.

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
KOiK00059.
OMAiMKDEDWN.
PhylomeDBiP0A2C9.

Enzyme and pathway databases

UniPathwayiUPA00094.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFABG_SALTY
AccessioniPrimary (citable) accession number: P0A2C9
Secondary accession number(s): O85141
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Calcium ions stabilize the structure, and may inhibit FabG activity by obstructing access to the active site.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.