P0A292 (DMA_SALTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA adenine methylase
EC=2.1.1.72

Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.StyDam

Gene names

Name:	dam
Ordered Locus Names:	STY4312, t4022

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	DNA adenine methylase		PRO_0000087993
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	14	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	54	1	S-adenosyl-L-methionine By similarity
	Binding site	181	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A292 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 900F4B77D827C3A8	FASTA	278	32,027
	10 20 30 40 50 60 MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY ILADINSDLI 70 80 90 100 110 120 SLYNIVKLRT DEYVQASREL FMPETNQAEV YYQLREEFNT CQDPFRRAVL FLYLNRYGYN 130 140 150 160 170 180 GLCRYNLRGE FNVPFGRYKR PYFPEAELYH FAEKAQNAFF YCESYADSMA RADKSSVVYC 190 200 210 220 230 240 DPPYAPLSAT ANFTAYHTNS FSLTQQAHLA EIAENLVSNR IPVLISNHDT ALTREWYQLA 250 260 270 KLHVVKVRRS ISSNGGTRKK VDELLALYQP GVATPARK

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL627281 Genomic DNA. Translation: CAD08130.1. AE014613 Genomic DNA. Translation: AAO71492.1.
RefSeq	NP_807632.1. NC_004631.1.
3D structure databases
ProteinModelPortal	P0A292.
SMR	P0A292. Positions 3-270.
ModBase	Search...
Protein-protein interaction databases
STRING	220341.STY4312.
Protein family/group databases
REBASE	5691. M.StyCDamP.
Proteomic databases
PRIDE	P0A292.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO71492; AAO71492; t4022. CAD08130; CAD08130; CAD08130.
GeneID	1070050.
KEGG	stt:t4022.
PATRIC	18546847. VBISalEnt120419_4407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0338.
HOGENOM	HOG000281348.
KO	K06223.
OMA	KARRTIS.
ProtClustDB	PRK10904.
Family and domain databases
Gene3D	1.10.1020.10. 1 hit.
InterPro	IPR023095. Ade_MeTrfase_dom_2. IPR002052. DNA_methylase_N6_adenine_CS. IPR012263. M_m6A_EcoRV. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PIRSF	PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DMA_SALTI

Accession

Primary (citable) accession number: P0A292
Secondary accession number(s): P55893

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program