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Protein

DNA adenine methylase

Gene

dam

Organism
Salmonella typhi
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101S-adenosyl-L-methionineBy similarity
Binding sitei14 – 141S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei54 – 541S-adenosyl-L-methionineBy similarity
Binding sitei181 – 1811S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi5691. M.StyCDamP.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.StyDam
Gene namesi
Name:dam
Ordered Locus Names:STY4312, t4022
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000002670 Componenti: Chromosome UP000000541 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278DNA adenine methylasePRO_0000087993Add
BLAST

Proteomic databases

PRIDEiP0A292.

Interactioni

Protein-protein interaction databases

STRINGi220341.STY4312.

Structurei

3D structure databases

ProteinModelPortaliP0A292.
SMRiP0A292. Positions 3-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0338.
HOGENOMiHOG000281348.
KOiK06223.
OMAiMNRHGFN.
OrthoDBiEOG6CS08V.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A292-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY
60 70 80 90 100
ILADINSDLI SLYNIVKLRT DEYVQASREL FMPETNQAEV YYQLREEFNT
110 120 130 140 150
CQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKR PYFPEAELYH
160 170 180 190 200
FAEKAQNAFF YCESYADSMA RADKSSVVYC DPPYAPLSAT ANFTAYHTNS
210 220 230 240 250
FSLTQQAHLA EIAENLVSNR IPVLISNHDT ALTREWYQLA KLHVVKVRRS
260 270
ISSNGGTRKK VDELLALYQP GVATPARK
Length:278
Mass (Da):32,027
Last modified:March 15, 2005 - v1
Checksum:i900F4B77D827C3A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL513382 Genomic DNA. Translation: CAD08130.1.
AE014613 Genomic DNA. Translation: AAO71492.1.
RefSeqiNP_458420.1. NC_003198.1.

Genome annotation databases

EnsemblBacteriaiAAO71492; AAO71492; t4022.
CAD08130; CAD08130; CAD08130.
GeneIDi1250529.
KEGGisty:STY4312.
PATRICi18546847. VBISalEnt120419_4407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL513382 Genomic DNA. Translation: CAD08130.1.
AE014613 Genomic DNA. Translation: AAO71492.1.
RefSeqiNP_458420.1. NC_003198.1.

3D structure databases

ProteinModelPortaliP0A292.
SMRiP0A292. Positions 3-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220341.STY4312.

Protein family/group databases

REBASEi5691. M.StyCDamP.

Proteomic databases

PRIDEiP0A292.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO71492; AAO71492; t4022.
CAD08130; CAD08130; CAD08130.
GeneIDi1250529.
KEGGisty:STY4312.
PATRICi18546847. VBISalEnt120419_4407.

Phylogenomic databases

eggNOGiCOG0338.
HOGENOMiHOG000281348.
KOiK06223.
OMAiMNRHGFN.
OrthoDBiEOG6CS08V.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CT18.
  2. "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18."
    Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R.
    J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700931 / Ty2.

Entry informationi

Entry nameiDMA_SALTI
AccessioniPrimary (citable) accession number: P0A292
Secondary accession number(s): P55893
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: May 27, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.