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P0A291

- DMA_SALTY

UniProt

P0A291 - DMA_SALTY

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Protein
DNA adenine methylase
Gene
dam, STM3484
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101S-adenosyl-L-methionine By similarity
Binding sitei14 – 141S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei54 – 541S-adenosyl-L-methionine By similarity
Binding sitei181 – 1811S-adenosyl-L-methionine By similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSENT99287:GCTI-3506-MONOMER.

Protein family/group databases

REBASEi2891. M.StyADam.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.StyDam
Gene namesi
Name:dam
Ordered Locus Names:STM3484
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278DNA adenine methylase
PRO_0000087994Add
BLAST

Proteomic databases

PaxDbiP0A291.
PRIDEiP0A291.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM3484.

Structurei

3D structure databases

ProteinModelPortaliP0A291.
SMRiP0A291. Positions 3-270.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0338.
HOGENOMiHOG000281348.
KOiK06223.
OMAiKFTREIY.
OrthoDBiEOG6CS08V.
PhylomeDBiP0A291.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A291-1 [UniParc]FASTAAdd to Basket

« Hide

MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY    50
ILADINSDLI SLYNIVKLRT DEYVQASREL FMPETNQAEV YYQLREEFNT 100
CQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKR PYFPEAELYH 150
FAEKAQNAFF YCESYADSMA RADKSSVVYC DPPYAPLSAT ANFTAYHTNS 200
FSLTQQAHLA EIAENLVSNR IPVLISNHDT ALTREWYQLA KLHVVKVRRS 250
ISSNGGTRKK VDELLALYQP GVATPARK 278
Length:278
Mass (Da):32,027
Last modified:March 15, 2005 - v1
Checksum:i900F4B77D827C3A8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti249 – 2491R → P in AAB19116. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U76993 Genomic DNA. Translation: AAB19116.1.
AE006468 Genomic DNA. Translation: AAL22346.1.
RefSeqiNP_462387.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL22346; AAL22346; STM3484.
GeneIDi1255007.
KEGGistm:STM3484.
PATRICi32385769. VBISalEnt20916_3682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U76993 Genomic DNA. Translation: AAB19116.1 .
AE006468 Genomic DNA. Translation: AAL22346.1 .
RefSeqi NP_462387.1. NC_003197.1.

3D structure databases

ProteinModelPortali P0A291.
SMRi P0A291. Positions 3-270.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM3484.

Protein family/group databases

REBASEi 2891. M.StyADam.

Proteomic databases

PaxDbi P0A291.
PRIDEi P0A291.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL22346 ; AAL22346 ; STM3484 .
GeneIDi 1255007.
KEGGi stm:STM3484.
PATRICi 32385769. VBISalEnt20916_3682.

Phylogenomic databases

eggNOGi COG0338.
HOGENOMi HOG000281348.
KOi K06223.
OMAi KFTREIY.
OrthoDBi EOG6CS08V.
PhylomeDBi P0A291.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-3506-MONOMER.

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProi IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A temperature-sensitive DNA adenine methyltransferase mutant of Salmonella typhimurium."
    Brawer R., Batista F.D., Burrone O.R., Sordelli D.O., Cerquetti M.C.
    Arch. Microbiol. 169:530-533(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiDMA_SALTY
AccessioniPrimary (citable) accession number: P0A291
Secondary accession number(s): P55893
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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