Reviewed, UniProtKB/Swiss-Prot P0A291 (DMA_SALTY)

Names and origin

Protein names

Recommended name:
    DNA adenine methylase
    EC=2.1.1.72
Alternative name(s):
    M.StyDam
    Deoxyadenosyl-methyltransferase
    DNA adenine methyltransferase

Gene names

Name:	dam
Ordered Locus Names:	STM3484

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	DNA adenine methylase		PRO_0000087994
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	14	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	54	1	S-adenosyl-L-methionine By similarity
	Binding site	181	1	S-adenosyl-L-methionine By similarity
Experimental info
	Sequence conflict	249	1	R → P in AAB19116. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A291-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 900F4B77D827C3A8	FASTA	278	32,027
	10 20 30 40 50 60 MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY ILADINSDLI 70 80 90 100 110 120 SLYNIVKLRT DEYVQASREL FMPETNQAEV YYQLREEFNT CQDPFRRAVL FLYLNRYGYN 130 140 150 160 170 180 GLCRYNLRGE FNVPFGRYKR PYFPEAELYH FAEKAQNAFF YCESYADSMA RADKSSVVYC 190 200 210 220 230 240 DPPYAPLSAT ANFTAYHTNS FSLTQQAHLA EIAENLVSNR IPVLISNHDT ALTREWYQLA 250 260 270 KLHVVKVRRS ISSNGGTRKK VDELLALYQP GVATPARK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A temperature-sensitive DNA adenine methyltransferase mutant of Salmonella typhimurium." Brawer R., Batista F.D., Burrone O.R., Sordelli D.O., Cerquetti M.C. Arch. Microbiol. 169:530-533(1998) [PubMed: 9575240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U76993 Genomic DNA. Translation: AAB19116.1. AE006468 Genomic DNA. Translation: AAL22346.1.
RefSeq	NP_462387.1.
3D structure databases
SMR	P0A291. Positions 3-270.
ModBase	Search...
Protein family/group databases
REBASE	2891. M.StyDam. 5605. M.StyLT2Dam.
Proteomic databases
PRIDE	P0A291.
Genome annotation databases
GeneID	1255007.
GenomeReviews	Gene locus STM3484 in contig AE006468_GR.
KEGG	stm:STM3484.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG586381.
OMA	WAGGKFR.
Enzyme and pathway databases
BioCyc	STYP99287:STM3484-MONOMER.
BRENDA	2.1.1.72. 2.
Family and domain databases
InterPro	IPR002294. D12N6_MeTrfase. IPR012326. Dam. IPR002052. DNA_methylase_N6_adenine_CS. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DMA_SALTY

Accession

Primary (citable) accession number: P0A291
Secondary accession number(s): P55893

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)