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P0A291 (DMA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA adenine methylase

EC=2.1.1.72
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.StyDam
Gene names
Name:dam
Ordered Locus Names:STM3484
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length278 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 278278DNA adenine methylase
PRO_0000087994

Sites

Binding site101S-adenosyl-L-methionine By similarity
Binding site141S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site541S-adenosyl-L-methionine By similarity
Binding site1811S-adenosyl-L-methionine By similarity

Experimental info

Sequence conflict2491R → P in AAB19116. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P0A291 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 900F4B77D827C3A8

FASTA27832,027
        10         20         30         40         50         60 
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY ILADINSDLI 

        70         80         90        100        110        120 
SLYNIVKLRT DEYVQASREL FMPETNQAEV YYQLREEFNT CQDPFRRAVL FLYLNRYGYN 

       130        140        150        160        170        180 
GLCRYNLRGE FNVPFGRYKR PYFPEAELYH FAEKAQNAFF YCESYADSMA RADKSSVVYC 

       190        200        210        220        230        240 
DPPYAPLSAT ANFTAYHTNS FSLTQQAHLA EIAENLVSNR IPVLISNHDT ALTREWYQLA 

       250        260        270 
KLHVVKVRRS ISSNGGTRKK VDELLALYQP GVATPARK 

« Hide

References

« Hide 'large scale' references
[1]"A temperature-sensitive DNA adenine methyltransferase mutant of Salmonella typhimurium."
Brawer R., Batista F.D., Burrone O.R., Sordelli D.O., Cerquetti M.C.
Arch. Microbiol. 169:530-533(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U76993 Genomic DNA. Translation: AAB19116.1.
AE006468 Genomic DNA. Translation: AAL22346.1.
RefSeqNP_462387.1. NC_003197.1.

3D structure databases

ProteinModelPortalP0A291.
SMRP0A291. Positions 3-270.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM3484.

Protein family/group databases

REBASE2891. M.StyADam.

Proteomic databases

PaxDbP0A291.
PRIDEP0A291.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL22346; AAL22346; STM3484.
GeneID1255007.
KEGGstm:STM3484.
PATRIC32385769. VBISalEnt20916_3682.

Phylogenomic databases

eggNOGCOG0338.
HOGENOMHOG000281348.
KOK06223.
OMALYMNRHG.
OrthoDBEOG6CS08V.
ProtClustDBPRK10904.

Enzyme and pathway databases

BioCycSENT99287:GCTI-3506-MONOMER.

Family and domain databases

Gene3D1.10.1020.10. 1 hit.
InterProIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSPR00505. D12N6MTFRASE.
TIGRFAMsTIGR00571. dam. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDMA_SALTY
AccessionPrimary (citable) accession number: P0A291
Secondary accession number(s): P55893
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 13, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries