P0A287 (RF1_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide chain release factor 1
Short name=RF-1

Gene names

Name:	prfA
Ordered Locus Names:	STM1776

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA By similarity. HAMAP MF_00093
Subcellular location	Cytoplasm HAMAP MF_00093.
Post-translational modification	Methylated by PrmC. Methylation increases the termination efficiency of RF1 By similarity. HAMAP MF_00093
Sequence similarities	Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	translation release factor activity, codon specific Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Peptide chain release factor 1 HAMAP MF_00093		PRO_0000177734
Amino acid modifications
	Modified residue	235	1	N5-methylglutamine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A287 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 67AF15BF7ADB6755	FASTA	360	40,461
	10 20 30 40 50 60 MKPSIVAKLE ALHERHEEVQ ALLGDAGIIA DQDRFRALSR EYAQLSDVSR CFTDWQQVQD 70 80 90 100 110 120 DIETAQMMLD DPEMREMAQE ELREAKEKSE QLEQQLQVLL LPKDPDDERN AFLEVRAGTG 130 140 150 160 170 180 GDEAALFAGD LFRMYSRYAE ARRWRVEIMS MSEGEHGGYK EIIAKISGDG VYGRLKFESG 190 200 210 220 230 240 GHRVQRVPAT ESQGRIHTSA CTVAVMPELP EAELPDINPA DLRIDTFRSS GAGGQHVNTT 250 260 270 280 290 300 DSAIRITHLP TGIVVECQDE RSQHKNKAKA LSVLGARIHA AETAKRQQAE ASTRRNLLGS 310 320 330 340 350 360 GDRSDRNRTY NFPQGRVTDH RINLTLYRLD ETMEGKLDML IEPIVQEHQA DLLAALSEQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, genetic characterization, and nucleotide sequence of the hemA-prfA operon of Salmonella typhimurium." Elliott T. J. Bacteriol. 171:3948-3960(1989) [PubMed: 2544564] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04243 Genomic DNA. Translation: AAA88611.1. AE006468 Genomic DNA. Translation: AAL20691.1.
PIR	B32890.
RefSeq	NP_460732.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P0A287.
SMR	P0A287. Positions 7-354.
ModBase	Search...
Proteomic databases
PRIDE	P0A287.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1253295.
GenomeReviews	Gene locus STM1776 in contig AE006468_GR.
KEGG	stm:STM1776.
PATRIC	32382065. VBISalEnt20916_1871.
Phylogenomic databases
HOGENOM	HBG629764.
OMA	NESEQGG.
ProtClustDB	PRK00591.
Enzyme and pathway databases
BioCyc	STYP99287:STM1776-MONOMER.
Family and domain databases
HAMAP	MF_00093. Rel_fac_1. [Tree]
InterPro	IPR005139. PCRF. IPR000352. Pep_chain_release_fac_I_II. IPR004373. PrfA. [Graphical view]
KO	K02835.
PANTHER	PTHR11075:SF9. PrfA. 1 hit.
Pfam	PF03462. PCRF. 1 hit. PF00472. RF-1. 1 hit. [Graphical view]
SMART	SM00937. PCRF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00019. PrfA. 1 hit.
PROSITE	PS00745. RF_PROK_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RF1_SALTY

Accession

Primary (citable) accession number: P0A287
Secondary accession number(s): P13654

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program