P0A277 (XGPT_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xanthine phosphoribosyltransferase
EC=2.4.2.22

Alternative name(s):
Xanthine-guanine phosphoribosyltransferase
Short name=XGPRT

Gene names

Name:	gpt
Ordered Locus Names:	STM0317

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts on guanine, xanthine and to a lesser extent hypoxanthine. HAMAP-Rule MF_01903
Catalytic activity	XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine. HAMAP-Rule MF_01903
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; XMP biosynthesis via salvage pathway; XMP from xanthine: step 1/1. HAMAP-Rule MF_01903
Subunit structure	Homotetramer By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_01903.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. XGPT subfamily.

Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	XMP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway purine ribonucleoside salvage Inferred from electronic annotation. Source: HAMAP
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP xanthine phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Xanthine phosphoribosyltransferase HAMAP-Rule MF_01903		PRO_0000139686
Regions
	Region	37 – 38	2	5-phosphoribose 1-diphosphate binding By similarity
	Region	92 – 96	5	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Metal binding	89	1	Magnesium By similarity
	Binding site	69	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	92	1	Xanthine By similarity
	Binding site	135	1	Xanthine; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A277 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: EE42974303E6900D	FASTA	152	16,970
	10 20 30 40 50 60 MSEKYVVTWD MLQIHARKLA SRLMPSEQWK GIIAVSRGGL VPGALLAREL GIRHVDTVCI 70 80 90 100 110 120 SSYDHDNQRE LKVLKRAEGD GEGFIVIDDL VDTGGTAVAI REMYPKAHFV TIFAKPAGRP 130 140 150 LVDDYVIDIP QNTWIEQPWD MGVVFVPPIS GR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Bryant D.W., Rossetto F.E., O'Reilly C., Nieboer E., Turnbull J. Submitted (NOV-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Regionally-targeted mutagenesis by metabolically-activated steviol: DNA sequence analysis of steviol-induced mutants of guanine phosphoribosyltransferase (gpt) gene of Salmonella typhimurium TM677." Matsui M., Sofuni T., Nohmi T. Mutagenesis 11:565-572(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29631 / TA 1538.
[3]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63336 Genomic DNA. Translation: CAA44936.1. U28239 Genomic DNA. Translation: AAC01774.1. AE006468 Genomic DNA. Translation: AAL19273.1.
PIR	S18888.
RefSeq	NP_459314.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P0A277.
SMR	P0A277. Positions 3-152.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM0317.
Proteomic databases
PRIDE	P0A277.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL19273; AAL19273; STM0317.
GeneID	1251836.
KEGG	stm:STM0317.
PATRIC	32378949. VBISalEnt20916_0337.
Phylogenomic databases
HOGENOM	HOG000226805.
KO	K00769.
OMA	PKAHFAT.
ProtClustDB	PRK09177.
Enzyme and pathway databases
UniPathway	UPA00602; UER00658.
Family and domain databases
HAMAP	MF_01903. XGPRT.
InterPro	IPR000836. PRibTrfase_dom. IPR023747. Xanthine_Guanine_PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XGPT_SALTY

Accession

Primary (citable) accession number: P0A277
Secondary accession number(s): P26972

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program