P0A270 (PPX_SALTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exopolyphosphatase
Short name=ExopolyPase
EC=3.6.1.11

Alternative name(s):
Metaphosphatase

Gene names

Name:	ppx
Ordered Locus Names:	STY2743, t0355

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate By similarity.
Catalytic activity	(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the gppA/ppx family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	polyphosphate catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exopolyphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 513	512	Exopolyphosphatase		PRO_0000194304

Sequences

	Sequence		Length	Mass (Da)
	P0A270 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 02ACBA7895F2ED6D	FASTA	513	58,145
	10 20 30 40 50 60 MPIYDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG LGADNKLSEE 70 80 90 100 110 120 AMERGLSCLS LFAERLQGFS PSSVCIVGTH TLRQAQNAAD FLKRAEKVIP YPIEIISGNE 130 140 150 160 170 180 EARLIFMGVE HTQPEKGRKL VIDIGGGSTE LVIGENFEPR LVESRRMGCV SFAQLYFPGG 190 200 210 220 230 240 VINKENFQRA RMAAAQKLET LTWQYRIQGW NVAMGASGTI KAAHEVLLAL GEKDGFITPE 250 260 270 280 290 300 RLDKLKSEVL KHRSFNALSL PGLSEERKAV FVPGLAILCG VFDALAIREL RLSDGALREG 310 320 330 340 350 360 VLYEMEGRFR HQDVRSRTAK SLANQYNIDR EQARRVLETT MQMYEQWQAQ QPKLAHPQLE 370 380 390 400 410 420 ALLRWAAMLH EVGLNINHSG LHRHSAYILQ HSDLPGFNQE QQMMMATLVR YHRKAIKLDD 430 440 450 460 470 480 MPRFTLFKKK QYLPLIQLLR LGVLLNNQRQ ATTTPPTLRL TTDDSHWTLC FPHDWFSQNA 490 500 510 LVLLDLEKEQ QYWEAVTGWR LNIEEESSPE IAA

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL627275 Genomic DNA. Translation: CAD02704.1. AE014613 Genomic DNA. Translation: AAO68074.1.
RefSeq	NP_457037.1. NC_003198.1. NP_804225.1. NC_004631.1.
3D structure databases
ProteinModelPortal	P0A270.
SMR	P0A270. Positions 11-506.
ModBase	Search...
Proteomic databases
PRIDE	P0A270.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1067963. 1249057.
GenomeReviews	Gene locus t0355 in contig AE014613_GR. Gene locus STY2743 in contig AL513382_GR.
KEGG	stt:t0355. sty:STY2743.
PATRIC	18543480. VBISalEnt120419_2781.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG451789.
OMA	HKHSYYL.
ProtClustDB	PRK10854.
Enzyme and pathway databases
BioCyc	SENT209261:T0355-MONOMER. SENT220341:STY2743-MONOMER.
Family and domain databases
InterPro	IPR022371. Exopolyphosphatase. IPR003695. Ppx_GppA. [Graphical view]
KO	K01524.
Pfam	PF02541. Ppx-GppA. 1 hit. [Graphical view]
TIGRFAMs	TIGR03706. Exo_poly_only. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPX_SALTI

Accession

Primary (citable) accession number: P0A270
Secondary accession number(s): O86091

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program