P0A269 (PPX_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exopolyphosphatase
Short name=ExopolyPase
EC=3.6.1.11

Alternative name(s):
Metaphosphatase

Gene names

Name:	ppx
Ordered Locus Names:	STM2502

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate By similarity.
Catalytic activity	(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the GppA/Ppx family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	polyphosphate catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	exopolyphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 513	512	Exopolyphosphatase		PRO_0000194305

Sequences

	Sequence		Length	Mass (Da)
	P0A269 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 02ACBA7895F2ED6D	FASTA	513	58,145
	10 20 30 40 50 60 MPIYDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG LGADNKLSEE 70 80 90 100 110 120 AMERGLSCLS LFAERLQGFS PSSVCIVGTH TLRQAQNAAD FLKRAEKVIP YPIEIISGNE 130 140 150 160 170 180 EARLIFMGVE HTQPEKGRKL VIDIGGGSTE LVIGENFEPR LVESRRMGCV SFAQLYFPGG 190 200 210 220 230 240 VINKENFQRA RMAAAQKLET LTWQYRIQGW NVAMGASGTI KAAHEVLLAL GEKDGFITPE 250 260 270 280 290 300 RLDKLKSEVL KHRSFNALSL PGLSEERKAV FVPGLAILCG VFDALAIREL RLSDGALREG 310 320 330 340 350 360 VLYEMEGRFR HQDVRSRTAK SLANQYNIDR EQARRVLETT MQMYEQWQAQ QPKLAHPQLE 370 380 390 400 410 420 ALLRWAAMLH EVGLNINHSG LHRHSAYILQ HSDLPGFNQE QQMMMATLVR YHRKAIKLDD 430 440 450 460 470 480 MPRFTLFKKK QYLPLIQLLR LGVLLNNQRQ ATTTPPTLRL TTDDSHWTLC FPHDWFSQNA 490 500 510 LVLLDLEKEQ QYWEAVTGWR LNIEEESSPE IAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Polyphosphate kinase (ppk) and exopolyphosphatase (ppx) genes in Salmonella typhimurium." Kim K.S., Fraley C.D., Kornberg A. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WRAY.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF085682 Genomic DNA. Translation: AAC34891.1. AE006468 Genomic DNA. Translation: AAL21396.1.
RefSeq	NP_461437.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P0A269.
SMR	P0A269. Positions 11-506.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM2502.
Proteomic databases
PaxDb	P0A269.
PRIDE	P0A269.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL21396; AAL21396; STM2502.
GeneID	1254024.
KEGG	stm:STM2502.
PATRIC	32383637. VBISalEnt20916_2641.
Phylogenomic databases
eggNOG	COG0248.
HOGENOM	HOG000258672.
KO	K01524.
OMA	IIGQRFE.
ProtClustDB	PRK10854.
Enzyme and pathway databases
BioCyc	SENT99287:GCTI-2518-MONOMER.
Family and domain databases
InterPro	IPR022371. Exopolyphosphatase. IPR003695. Ppx_GppA. [Graphical view]
Pfam	PF02541. Ppx-GppA. 1 hit. [Graphical view]
TIGRFAMs	TIGR03706. exo_poly_only. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPX_SALTY

Accession

Primary (citable) accession number: P0A269
Secondary accession number(s): O86091

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program