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Protein

Alkyl hydroperoxide reductase C

Gene

ahpC

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.2 Publications

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by AhpF.1 Publication1 Publication

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.1 Publication

Kineticsi

kcat is 55.1 sec(-1) with H2O2 as substrate.1 Publication
  1. KM=1.4 µM for H2O21 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei47Cysteine sulfenic acid (-SOH) intermediate1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAntioxidant, Oxidoreductase, Peroxidase

    Enzyme and pathway databases

    BRENDAi1.11.1.15. 5542.

    Protein family/group databases

    PeroxiBasei4829. SetypAhpC.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkyl hydroperoxide reductase C1 Publication (EC:1.11.1.151 Publication)
    Alternative name(s):
    Alkyl hydroperoxide reductase protein C221 Publication
    Peroxiredoxin
    Thioredoxin peroxidase
    Gene namesi
    Name:ahpC1 Publication
    Ordered Locus Names:STM0608
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
    Proteomesi
    • UP000001014 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm By similarity

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved2 Publications
    ChainiPRO_00001351202 – 187Alkyl hydroperoxide reductase CAdd BLAST186

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi47Interchain (with C-166); in linked formCombined sources1 Publication
    Disulfide bondi166Interchain (with C-47); in linked formCombined sources1 Publication

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP0A251.
    PRIDEiP0A251.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked, upon oxidation (PubMed:2643600, PubMed:8555198). 5 homodimers assemble to form a ring-like decamer (PubMed:11969410).3 Publications

    Protein-protein interaction databases

    STRINGi99287.STM0608.

    Structurei

    Secondary structure

    1187
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi12 – 17Combined sources6
    Beta strandi20 – 25Combined sources6
    Helixi26 – 29Combined sources4
    Beta strandi32 – 38Combined sources7
    Beta strandi42 – 45Combined sources4
    Helixi46 – 56Combined sources11
    Helixi58 – 63Combined sources6
    Beta strandi66 – 74Combined sources9
    Helixi76 – 85Combined sources10
    Helixi89 – 91Combined sources3
    Beta strandi94 – 98Combined sources5
    Helixi103 – 107Combined sources5
    Turni113 – 115Combined sources3
    Beta strandi116 – 118Combined sources3
    Beta strandi120 – 125Combined sources6
    Beta strandi129 – 137Combined sources9
    Turni139 – 141Combined sources3
    Helixi145 – 160Combined sources16
    Beta strandi161 – 163Combined sources3
    Turni182 – 186Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1N8JX-ray2.17A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T2-187[»]
    1YEPX-ray2.50A/B/C/D/E2-187[»]
    1YEXX-ray2.30A/B/C/D/E2-187[»]
    1YF0X-ray2.50A/B/C/D/E2-187[»]
    1YF1X-ray2.60A/B/C/D/E/F/G/H/I/J2-187[»]
    3EMPX-ray4.00A/B/C/D/E2-187[»]
    4MA9X-ray1.82A/B/C/D/E2-187[»]
    4MABX-ray1.90A/B/C/D/E2-187[»]
    4XRAX-ray1.75A/B/C/D/E2-187[»]
    4XRDX-ray2.30A/B/C/D/E2-187[»]
    4XS1X-ray2.10A/B/C/D/E2-187[»]
    4XS4X-ray2.65A/B/C/D/E2-187[»]
    4XS6X-ray3.35A/B/C/D/E2-187[»]
    4XS8X-ray1.90A/B/C/D/E2-187[»]
    4XTSX-ray2.70A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T2-187[»]
    ProteinModelPortaliP0A251.
    SMRiP0A251.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A251.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini2 – 157ThioredoxinPROSITE-ProRule annotationAdd BLAST156

    Sequence similaritiesi

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiENOG4105D3R. Bacteria.
    COG0450. LUCA.
    HOGENOMiHOG000022343.
    KOiK03386.
    OMAiCPANWEE.
    PhylomeDBiP0A251.

    Family and domain databases

    InterProiView protein in InterPro
    IPR017559. AhpC.
    IPR000866. AhpC/TSA.
    IPR024706. Peroxiredoxin_AhpC-typ.
    IPR019479. Peroxiredoxin_C.
    IPR036249. Thioredoxin-like_sf.
    IPR013766. Thioredoxin_domain.
    PANTHERiPTHR10681:SF121. PTHR10681:SF121. 1 hit.
    PfamiView protein in Pfam
    PF10417. 1-cysPrx_C. 1 hit.
    PF00578. AhpC-TSA. 1 hit.
    PIRSFiPIRSF000239. AHPC. 1 hit.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    TIGRFAMsiTIGR03137. AhpC. 1 hit.
    PROSITEiView protein in PROSITE
    PS51352. THIOREDOXIN_2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A251-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSLINTKIKP FKNQAFKNGE FIEVTEKDTE GRWSVFFFYP ADFTFVCPTE
    60 70 80 90 100
    LGDVADHYEE LQKLGVDVYS VSTDTHFTHK AWHSSSETIA KIKYAMIGDP
    110 120 130 140 150
    TGALTRNFDN MREDEGLADR ATFVVDPQGI IQAIEVTAEG IGRDASDLLR
    160 170 180
    KIKAAQYVAA HPGEVCPAKW KEGEATLAPS LDLVGKI
    Length:187
    Mass (Da):20,747
    Last modified:January 23, 2007 - v2
    Checksum:i83B251C74DE9860D
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti2S → G AA sequence (PubMed:2643600).Curated1
    Sequence conflicti5N → D AA sequence (PubMed:2643600).Curated1
    Sequence conflicti14Q → N AA sequence (PubMed:2643600).Curated1
    Sequence conflicti17K → H AA sequence (PubMed:2643600).Curated1
    Sequence conflicti20E → H AA sequence (PubMed:2643600).Curated1
    Sequence conflicti23E → S AA sequence (PubMed:2643600).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J05478 Unassigned DNA. Translation: AAA16431.1.
    AE006468 Genomic DNA. Translation: AAL19559.1.
    PIRiA35441.
    RefSeqiNP_459600.1. NC_003197.2.
    WP_000052802.1. NC_003197.2.

    Genome annotation databases

    EnsemblBacteriaiAAL19559; AAL19559; STM0608.
    GeneIDi1252128.
    KEGGistm:STM0608.
    PATRICifig|99287.12.peg.640.

    Similar proteinsi

    Entry informationi

    Entry nameiAHPC_SALTY
    AccessioniPrimary (citable) accession number: P0A251
    Secondary accession number(s): P19479
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: January 23, 2007
    Last modified: October 25, 2017
    This is version 98 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families