Reviewed, UniProtKB/Swiss-Prot P0A231 (PA1_SALTY)

Names and origin

Protein names

Recommended name:
    Phospholipase A1
    EC=3.1.1.32
    EC=3.1.1.4
Alternative name(s):
    Detergent-resistant phospholipase A
      Short name=DR-phospholipase A
    Phosphatidylcholine 1-acylhydrolase
    Outer membrane phospholipase A
      Short name=OM PLA

Gene names

Name:	pldA
Ordered Locus Names:	STM3957
ORF Names:	STMD1.33

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities.
Catalytic activity	Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate. Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Calcium.
Subcellular location	Cell outer membrane. Note: One of the very few enzymes located there.
Sequence similarities	Belongs to the phospholipase A1 family.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal
Ligand	Calcium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A1 activity Inferred from electronic annotation. Source: EC phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Chain	21 – 289	269	Phospholipase A1		PRO_0000021989
Sites
	Active site	164	1	By similarity
Experimental info
	Sequence conflict	155	1	D → H in CAA54222. Ref.1
	Sequence conflict	174	1	S → T in CAA54222. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A231-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: DA97F5E1651C49C6	FASTA	289	32,967
	10 20 30 40 50 60 MRAILRGLLP ATLLPLAAYA QEATIKEVHD APAVRGSIIA NMLQEHDNPF TLYPYDTNYL 70 80 90 100 110 120 IYTNTSDLNK EAISTYNWSE NARKDEVKFQ LSLAFPLWRG ILGPNSVLGA SYTQKSWWQL 130 140 150 160 170 180 SNSKESSPFR ETNYEPQLFL GFATDYRFAG WTLRDVEMGY NHDSNGRSDP TSRSWNRLYT 190 200 210 220 230 240 RLMAENGNWL VEVKPWYVIG STDDNPDITK YMGYYQLKIG YHLGEAVLSA KGQYNWNTGY 250 260 270 280 GGAEVGLSYP VTKHVRLYTQ VYSGYGESLI DYNFNQTRVG VGVMLNDIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of enterobacterial pldA genes encoding outer membrane phospholipase A." Brok R.G.P.M., Brinkman E., van Boxtel R., Bekkers A.C.A.P., Verheij H.M., Tommassen J. J. Bacteriol. 176:861-870(1994) [PubMed: 8300539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X76900 Genomic DNA. Translation: CAA54222.1. AF233324 Genomic DNA. Translation: AAF33435.1. AE008884 Genomic DNA. Translation: AAL22801.1.
PIR	A36971.
RefSeq	NP_462842.1.
3D structure databases
HSSP	HSSP built from PDB template 1QD6 based on UniProtKB P00631.
SMR	P0A231. Positions 28-289.
ModBase	Search...
Genome annotation databases
GeneID	1255483.
GenomeReviews	Gene locus STM3957 in contig AE006468_GR.
KEGG	stm:STM3957.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A231.
OMA	P0A231. YTRLMAQ.
Enzyme and pathway databases
BioCyc	STYP99287:STM3957-MON.
BRENDA	3.1.1.32. 2. 3.1.1.4. 2.
Family and domain databases
InterPro	IPR003187. PLA1. [Graphical view]
Gene3D	G3DSA:2.40.230.10. PLA1. 1 hit.
Pfam	PF02253. PLA1. 1 hit. [Graphical view]
PRINTS	PR01486. PHPHLIPASEA1.
ProtoNet	Search...

Entry information

Entry name

PA1_SALTY

Accession

Primary (citable) accession number: P0A231
Secondary accession number(s): P37442, Q9L6N9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)