Reviewed, UniProtKB/Swiss-Prot P0A229 (NIRD_SALTY)

Names and origin

Protein names

Recommended name:
    Nitrite reductase [NAD(P)H] small subunit
    EC=1.7.1.4

Gene names

Name:	nirD
Ordered Locus Names:	STM3475

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function	Required for activity of the reductase.
Catalytic activity	Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H.
Subunit structure	Associates with nirB.
Subcellular location	Cytoplasm.

Ontologies

Keywords
Biological process	Nitrate assimilation
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: InterPro nitrite reductase [NAD(P)H] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 108	108	Nitrite reductase [NAD(P)H] small subunit		PRO_0000096857
Experimental info
	Sequence conflict	76	1	R → P Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A229-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 375C6D13AFF98161	FASTA	108	12,097
	10 20 30 40 50 60 MSQWQNICKI DDILPGTGVC ALSGGEQVAI FRPYHSDQVF AISNIDPFFE ASVLSRGLIA 70 80 90 100 EHQGELWVAS PLKKQRFRLS DGLCMEDEQF SVKHYDARVK DGVVQLRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[2]	"High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor." Wu J.Y., Siegel L.M., Kredich N.M. J. Bacteriol. 173:325-333(1991) [PubMed: 1987123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 42-108. Strain: LT2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006468 Genomic DNA. Translation: AAL22337.1. M64606 Genomic DNA. No translation available.
RefSeq	NP_462378.1.
3D structure databases
SMR	P0A229. Positions 1-108.
ModBase	Search...
Proteomic databases
PRIDE	P0A229.
Genome annotation databases
GeneID	1254998.
GenomeReviews	Gene locus STM3475 in contig AE006468_GR.
KEGG	stm:STM3475.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG668715.
OMA	GVCALVE.
Enzyme and pathway databases
BioCyc	STYP99287:STM3475-MONOMER.
BRENDA	1.7.1.4. 2.
Family and domain databases
InterPro	IPR012748. Nitri_red_NirD. IPR017881. Nitrite_Rdtase_lsu_NirD_bac. IPR017941. Rieske_2Fe-2S. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
TIGRFAMs	TIGR02378. nirD_assim_sml. 1 hit.
PROSITE	PS51300. NIRD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIRD_SALTY

Accession

Primary (citable) accession number: P0A229
Secondary accession number(s): P40789

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)