Reviewed, UniProtKB/Swiss-Prot P0A1Y7 (NUOCD_SALTI)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit C/D
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit C/D
    NDH-1 subunit C/D

Gene names

Name:	nuoC
Synonyms:	nuoCD, nuoD
Ordered Locus Names:	STY2556, t0538

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01359
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits nuoB, CD, E, F, and G constitute the peripheral sector of the complex By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	In the N-terminal section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 596	595	NADH-quinone oxidoreductase subunit C/D HAMAP MF_01359		PRO_0000118679
Regions
	Region	2 – 186	185	NADH dehydrogenase I subunit C By similarity
	Region	210 – 596	387	NADH dehydrogenase I subunit D By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A1Y7-1 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 362B1FBD9A864F46	FASTA	596	68,402
	10 20 30 40 50 60 MTDLTAQDAA WSTRDHLDDP VIGELRNRFG PDAFTVQATR TGIPVVWVKR EQLLEVGDFL 70 80 90 100 110 120 KKLPKPYVML FDLHGMDERL RTHRDGLPAA DFSVFYHLIS IERNRDIMLK VALSENDLRV 130 140 150 160 170 180 PTFTKLFPNA NWYERETWEM FGIDIEGHPH LTRIMMPQTW EGHPLRKDYP ARATEFDPFE 190 200 210 220 230 240 LTKAKQDLEM EALTFKPEDW GMKRGTDNED FMFLNLGPNH PSAHGAFRII LQLDGEEIVD 250 260 270 280 290 300 CVPDIGYHHR GAEKMGERQS WHSYIPYTDR IEYLGGCVNE MPYVLAVEKL AGITVPDRVN 310 320 330 340 350 360 VIRVMLSELF RINSHLLYIS TFIQDVGAMT PVFFAFTDRQ KIYDLVEAIT GFRMHPAWFR 370 380 390 400 410 420 IGGVAHDLPR GWDRLLREFL EWMPKRLDSY EKAALRNTIL KGRSQGVAAY GAKEALEWGT 430 440 450 460 470 480 TGAGLRATGI DFDVRKWRPY SGYENFDFEV PVGGGVSDCY TRVMLKVEEL RQSLRILQQC 490 500 510 520 530 540 LDNMPEGPFK ADHPLTTPPP KERTLQHIET LITHFLQVSW GPVMPAQESF QMVEATKGIN 550 560 570 580 590 SYYLTSDGST MSYRTRVRTP SFAHLQQIPS AIRGSLVSDL IVYLGSIDFV MSDVDR

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL627274 Genomic DNA. Translation: CAD07558.1. AE014613 Genomic DNA. Translation: AAO68244.1.
RefSeq	NP_456868.1. NP_804395.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1069525. 1248881.
GenomeReviews	Gene locus t0538 in contig AE014613_GR. Gene locus STY2556 in contig AL513382_GR.
KEGG	stt:t0538. sty:STY2556.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A1Y7.
Enzyme and pathway databases
BioCyc	SENT209261:T0538-MON. SENT220341:STY2556-MON.
BRENDA	1.6.99.5. 3716.
Family and domain databases
HAMAP	MF_01359. [Tree]
InterPro	IPR010219. NADH_DH_1_dsu. IPR010218. NADH_DH_csu. IPR001135. NADH_Q_OxRdtase_suD. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_su-49kDa_CS. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit. TIGR01962. NuoD. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. False negative. PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOCD_SALTI

Accession

Primary (citable) accession number: P0A1Y7
Secondary accession number(s): P33902

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	July 22, 2008
Last modified:	June 16, 2009
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)