P0A1Y0 (MUTS_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA mismatch repair protein mutS

Gene names

Name:	mutS
Ordered Locus Names:	STM2909

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	855 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity. HAMAP MF_00096
Sequence similarities	Belongs to the DNA mismatch repair mutS family.
Sequence caution	The sequence AAA27167.1 differs from that shown. Reason: Frameshift at positions 353, 379, 394, 422 and 425. The sequence AAA80578.1 differs from that shown. Reason: Frameshift at positions 353, 379, 394, 422 and 425.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	mismatch repair Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW mismatched DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 855	855	DNA mismatch repair protein mutS HAMAP MF_00096		PRO_0000115132
Regions
	Nucleotide binding	616 – 623	8	ATP Potential
Experimental info
	Mutagenesis	622	1	K → A: Defective in mismatch repair; ATPase activity reduced 6-fold.
	Sequence conflict	361 – 362	2	TA → S Ref.1
	Sequence conflict	361 – 362	2	TA → S Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A1Y0 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 804BEB131BCD2F6F	FASTA	855	95,407
	10 20 30 40 50 60 MNESFDKDFS NHTPMMQQYL KLKAQHPEIL LFYRMGDFYE LFYDDAKRAS QLLDISLTKR 70 80 90 100 110 120 GASAGEPIPM AGIPHHAVEN YLAKLVNQGE SVAICEQIGD PATSKGPVER KVVRIVTPGT 130 140 150 160 170 180 ISDEALLQER QDNLLAAIWQ DGKGYGYATL DISSGRFRLS EPADRETMAA ELQRTNPAEL 190 200 210 220 230 240 LYAEDFAEMA LIEGRRGLRR RPLWEFEIDT ARQQLNLQFG TRDLVGFGVE NASRGLCAAG 250 260 270 280 290 300 CLLQYVKDTQ RTSLPHIRSI TMERQQDSII MDAATRRNLE ITQNLAGGVE NTLAAVLDCT 310 320 330 340 350 360 VTPMGSRMLK RWLHMPVRNT DILRERQQTI GALQDTVSEL QPVLRQVGDL ERILARLALR 370 380 390 400 410 420 TARPRDLARM RHAFQQLPEL HAQLETVDSA PVQALRKKMG DFAELRDLLE RAIIDAPPVL 430 440 450 460 470 480 VRDGGVIAPG YHEELDEWRA LADGATDYLD RLEIRERERT GLDTLKVGYN AVHGYYIQIS 490 500 510 520 530 540 RGQSHLAPIN YVRRQTLKNA ERYIIPELKE YEDKVLTSKG KALALEKQLY DELFDLLLPH 550 560 570 580 590 600 LADLQQSANA LAELDVLVNL AERAWTLNYT CPTFTDKPGI RITEGRHPVV EQVLNEPFIA 610 620 630 640 650 660 NPLNLSPQRR MLIITGPNMG GKSTYMRQTA LIALLAYIGS YVPAQNVEIG PIDRIFTRVG 670 680 690 700 710 720 AADDLASGRS TFMVEMTETA NILHNATENS LVLMDEIGRG TSTYDGLSLA WACAENLANK 730 740 750 760 770 780 IKALTLFATH YFELTQLPEK MEGVANVHLD ALEHGDTIAF MHSVQDGAAS KSYGLAVAAL 790 800 810 820 830 840 AGVPKEVIKR ARQKLRELES ISPNAAATQV DGTQMSLLAA PEETSPAVEA LENLDPDSLT 850 PRQALEWIYR LKSLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the Salmonella typhimurium mutS gene required for mismatch repair: homology of MutS and HexA of Streptococcus pneumoniae." Haber L.T., Pang P.P., Sobell D.I., Mankovich J.A., Walker G.C. J. Bacteriol. 170:197-202(1988) [PubMed: 3275609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5.
[2]	"A 40 kb chromosomal fragment encoding Salmonella typhimurium invasion genes is absent from the corresponding region of the Escherichia coli K-12 chromosome." Mills D.M., Bajaj V., Lee C.A. Mol. Microbiol. 15:749-759(1995) [PubMed: 7783645] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[4]	"Altering the conserved nucleotide binding motif in the Salmonella typhimurium MutS mismatch repair protein affects both its ATPase and mismatch binding activities." Haber L.T., Walker G.C. EMBO J. 10:2707-2715(1991) [PubMed: 1651234] [Abstract] Cited for: MUTAGENESIS, ATP-BINDING.
[5]	"Detecting frame shifts by amino acid sequence comparison." Claverie J.-M. J. Mol. Biol. 234:1140-1157(1993) [PubMed: 7903399] [Abstract] Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFT IN 394-425.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M18965 Genomic DNA. Translation: AAA27167.1. Frameshift. U16303 Genomic DNA. Translation: AAA80578.1. Frameshift. AE006468 Genomic DNA. Translation: AAL21789.1.
PIR	A28668.
RefSeq	NP_461830.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P0A1Y0.
SMR	P0A1Y0. Positions 7-802, 822-855.
ModBase	Search...
Proteomic databases
PRIDE	P0A1Y0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1254432.
GenomeReviews	Gene locus STM2909 in contig AE006468_GR.
KEGG	stm:STM2909.
PATRIC	32384502. VBISalEnt20916_3063.
Phylogenomic databases
HOGENOM	HBG735169.
OMA	DFFECFF.
ProtClustDB	PRK05399.
Enzyme and pathway databases
BioCyc	STYP99287:STM2909-MONOMER.
Family and domain databases
HAMAP	MF_00096. MutS. [Tree]
InterPro	IPR005748. DNA_mismatch_repair_MutS-1. IPR007695. DNA_mismatch_repair_MutS-lik_N. IPR000432. DNA_mismatch_repair_MutS_C. IPR007861. DNA_mismatch_repair_MutS_clamp. IPR007860. DNA_mismatch_repair_MutS_connt. IPR007696. DNA_mismatch_repair_MutS_core. IPR016151. DNA_mismatch_repair_MutS_N. [Graphical view]
Gene3D	G3DSA:3.30.420.110. DNA_mismatch_repair_MutS_connt. 1 hit. G3DSA:3.40.1170.10. DNA_mismatch_repair_MutS_N. 1 hit.
KO	K03555.
PANTHER	PTHR11361:SF34. PTHR11361:SF34. 1 hit.
Pfam	PF01624. MutS_I. 1 hit. PF05188. MutS_II. 1 hit. PF05192. MutS_III. 1 hit. PF05190. MutS_IV. 1 hit. PF00488. MutS_V. 1 hit. [Graphical view]
SMART	SM00534. MUTSac. 1 hit. SM00533. MUTSd. 1 hit. [Graphical view]
SUPFAM	SSF53150. DNA_mismatch_repair_MutS_connt. 1 hit. SSF55271. DNA_mismatch_repair_MutS_N. 1 hit. SSF48334. DNA_repair_MutS_domIII. 1 hit.
TIGRFAMs	TIGR01070. MutS1. 1 hit.
PROSITE	PS00486. DNA_MISMATCH_REPAIR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MUTS_SALTY

Accession

Primary (citable) accession number: P0A1Y0
Secondary accession number(s): P10339

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program