Reviewed, UniProtKB/Swiss-Prot P0A1X7 (AMPM_SALTI)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Synonyms:	pepM
Ordered Locus Names:	STY0238, t0216

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity. Binds 1 sodium ion per subunit. The sodium ion has a structural role By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 264	263	Methionine aminopeptidase		PRO_0000148951
Sites
	Metal binding	97	1	Cobalt 1 By similarity
	Metal binding	108	1	Cobalt 1 By similarity
	Metal binding	108	1	Cobalt 2 By similarity
	Metal binding	171	1	Cobalt 2 By similarity
	Metal binding	204	1	Cobalt 2 By similarity
	Metal binding	235	1	Cobalt 1 By similarity
	Metal binding	235	1	Cobalt 2 By similarity
	Binding site	79	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A1X7-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8563A54BBE98A860	FASTA	264	29,292
	10 20 30 40 50 60 MAISIKTSED IEKMRVAGRL AAEVLEMIEP YIKPGVTTGE LDRICNDYIV NEQHAISACL 70 80 90 100 110 120 GYHGYPKSVC ISINEVVCHG IPDDAKHLKD GDIVNIDVTV IKDEFHGDTS KMFIVGKPTI 130 140 150 160 170 180 LGERLCRVTQ ESLYLGIKMV KPGIRLRTIG AAIQKYAEGE GFSVVREYCG HGIGRGFHEE 190 200 210 220 230 240 PQVLHYDADD GGVVLQPGMT FTIEPMLNAG DYRIRTMKDG WTVKTKDRSL SAQYEHTIVV 250 260 TENGCEILTL RKDDTIPAII THDE

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL627265 Genomic DNA. Translation: CAD01369.1. AE014613 Genomic DNA. Translation: AAO67946.1.
RefSeq	NP_454822.1. NP_804097.1.
3D structure databases
HSSP	HSSP built from PDB template 3MAT based on UniProtKB P07906.
SMR	P0A1X7. Positions 2-264.
ModBase	Search...
Proteomic databases
PRIDE	P0A1X7.
Genome annotation databases
GeneID	1067809. 1246727.
GenomeReviews	Gene locus t0216 in contig AE014613_GR. Gene locus STY0238 in contig AL513382_GR.
KEGG	stt:t0216. sty:STY0238.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A1X7.
OMA	VIQKHAE.
Enzyme and pathway databases
BioCyc	SENT209261:T0216-MON. SENT220341:STY0238-MON.
BRENDA	3.4.11.18. 3716.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00500. met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_SALTI

Accession

Primary (citable) accession number: P0A1X7
Secondary accession number(s): P10882

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)