P0A1X6 (AMPM_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase
Short name=MAP
EC=3.4.11.18

Alternative name(s):
Peptidase M

Gene names

Name:	map
Synonyms:	pepM
Ordered Locus Names:	STM0215

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity. Binds 1 sodium ion per subunit. The sodium ion has a structural role By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 264	263	Methionine aminopeptidase		PRO_0000148952
Sites
	Metal binding	97	1	Cobalt 1 By similarity
	Metal binding	108	1	Cobalt 1 By similarity
	Metal binding	108	1	Cobalt 2 By similarity
	Metal binding	171	1	Cobalt 2 By similarity
	Metal binding	204	1	Cobalt 2 By similarity
	Metal binding	235	1	Cobalt 1 By similarity
	Metal binding	235	1	Cobalt 2 By similarity
	Binding site	79	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
Experimental info
	Sequence conflict	136 – 138	3	GIK → ALR AA sequence Ref.3
	Sequence conflict	145	1	R → N AA sequence Ref.3
	Sequence conflict	148	1	T → E AA sequence Ref.3
	Sequence conflict	159	1	G → A AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P0A1X6 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8563A54BBE98A860	FASTA	264	29,292
	10 20 30 40 50 60 MAISIKTSED IEKMRVAGRL AAEVLEMIEP YIKPGVTTGE LDRICNDYIV NEQHAISACL 70 80 90 100 110 120 GYHGYPKSVC ISINEVVCHG IPDDAKHLKD GDIVNIDVTV IKDEFHGDTS KMFIVGKPTI 130 140 150 160 170 180 LGERLCRVTQ ESLYLGIKMV KPGIRLRTIG AAIQKYAEGE GFSVVREYCG HGIGRGFHEE 190 200 210 220 230 240 PQVLHYDADD GGVVLQPGMT FTIEPMLNAG DYRIRTMKDG WTVKTKDRSL SAQYEHTIVV 250 260 TENGCEILTL RKDDTIPAII THDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of the Salmonella typhimurium pepM gene." Movva N.R., Semon D., Meyer C., Kawashima E., Wingfield P., Miller J.L., Miller C.G. Mol. Gen. Genet. 223:345-348(1990) [PubMed: 2250660] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	"Purification and characterization of a methionine-specific aminopeptidase from Salmonella typhimurium." Wingfield P., Graber P., Turcatti G., Movva N.R., Pelletier M., Craig S., Rose K., Miller C.G. Eur. J. Biochem. 180:23-32(1989) [PubMed: 2651123] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-264.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55778 Genomic DNA. Translation: CAA39298.1. AE006468 Genomic DNA. Translation: AAL19179.1.
PIR	S12027.
RefSeq	NP_459220.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P0A1X6.
SMR	P0A1X6. Positions 2-264.
ModBase	Search...
Protein family/group databases
MEROPS	M24.001.
Proteomic databases
PRIDE	P0A1X6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1251733.
GenomeReviews	Gene locus STM0215 in contig AE006468_GR.
KEGG	stm:STM0215.
PATRIC	32378717. VBISalEnt20916_0228.
Phylogenomic databases
HOGENOM	HBG299384.
OMA	ENGCEIM.
ProtClustDB	PRK05716.
Enzyme and pathway databases
BioCyc	STYP99287:STM0215-MONOMER.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
KO	K01265.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00500. Met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_SALTY

Accession

Primary (citable) accession number: P0A1X6
Secondary accession number(s): P10882

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program