Reviewed, UniProtKB/Swiss-Prot P0A1W3 (LEP_SALTI)

Names and origin

Protein names

Recommended name:
    Signal peptidase I
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	STY2828, t0275

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Signal peptidase I		PRO_0000109517
Regions
	Topological domain	1 – 3	3	Periplasmic Potential
	Transmembrane	4 – 22	19	Potential
	Topological domain	23 – 58	36	Cytoplasmic Potential
	Transmembrane	59 – 77	19	Potential
	Topological domain	78 – 324	247	Periplasmic Potential
Sites
	Active site	91	1	By similarity
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A1W3-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 40607CDA15779575	FASTA	324	35,778
	10 20 30 40 50 60 MANMFALILV IATLVTGILW CVDKFVFAPK RRARQAAAQT ASGDALDNAT LNKVAPKPGW 70 80 90 100 110 120 LETGASVFPV LAIVLIVRSF LYEPFQIPSG SMMPTLLIGD FILVEKFAYG IKDPIYQKTL 130 140 150 160 170 180 IETGHPKRGD IVVFKYPEDP KLDYIKRAVG LPGDKITYDP VAKEVTIQPG CSSGQACENA 190 200 210 220 230 240 LPVTYSNVEP SDFVQTFARR NGGEATSGFF EVPLNETKEN GIRLTERKET LGDVTHRILM 250 260 270 280 290 300 VPIAQDQLGM YYQQPGQPLA TWVVPPGQYF MMGDNRDNSA DSRYWGFVPE ANLVGKAVAI 310 320 WMSFDKQEGE WPTGVRLSRI GGIH

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL627275 Genomic DNA. Translation: CAD02784.1. AE014613 Genomic DNA. Translation: AAO68000.1.
RefSeq	NP_457111.1. NP_804151.1.
3D structure databases
HSSP	HSSP built from PDB template 1KN9 based on UniProtKB P00803.
SMR	P0A1W3. Positions 78-324.
ModBase	Search...
Proteomic databases
PRIDE	P0A1W3.
Genome annotation databases
GeneID	1067876. 1249137.
GenomeReviews	Gene locus t0275 in contig AE014613_GR. Gene locus STY2828 in contig AL513382_GR.
KEGG	stt:t0275. sty:STY2828.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A1W3.
OMA	VAVFKYP.
Enzyme and pathway databases
BioCyc	SENT209261:T0275-MON. SENT220341:STY2828-MON.
BRENDA	3.4.21.89. 3716.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR019533. Peptidase_S26_cons-reg. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. PF10502. Peptidase_S26. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_SALTI

Accession

Primary (citable) accession number: P0A1W3
Secondary accession number(s): P23697

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)